Seminal arginase activity in infertility

Elgün, Serenay; Kaçmaz, Murat; Şen, İ̇lker; Durak, İlker

doi:10.1007/s002400050004

Cited by 18 publications

(28 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Elgun et al (32) have shown that sperm cells from infertile men with oligospermia have a significantly higher arginase activity than controls. They reported a positive correlation between sperm motility and arginase activity in the infertile group in both seminal plasma and sperm cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality

Eskiocak

Gözen

Taşkıran

et al. 2006

Braz J Med Biol Res

View full text Add to dashboard Cite

It has been reported that mental stress causes abnormality of spermiogram parameters. We investigated the effect of psychological stress on the L-arginine-nitric oxide (NO) pathway. Semen samples were collected from 29 healthy fourth semester medical students just before (stress) and 3 months after (non-stress) the final examinations. Psychological stress was measured by the State Anxiety Inventory questionnaire. After standard semen analysis, arginase activity and NO concentration were measured spectrophotometrically in the seminal plasma. Measurements were made in duplicate. During the stress period, sperm concentration (41.28 ± 3.70 vs 77.62 ± 7.13 x 10 6 /mL), rapid progressive motility of spermatozoa (8.79 ± 1.66 vs 20.86 ± 1.63%) and seminal plasma arginase activity (0.12 ± 0.01 vs 0.22 ± 0.01 U/mL) were significantly lower than in the non-stress situation, whereas seminal plasma NO (17.28 ± 0.56 vs 10.02 ± 0.49 µmol/L) was higher compared to the non-stress period (P < 0.001 for all). During stress there was a negative correlation between NO concentration and sperm concentration, the percentage of rapid progressive motility and arginase activity (r = -0.622, P < 0.01; r = -0.425, P < 0.05 and r = -0.445, P < 0.05, respectively). These results indicate that psychological stress causes an increase of NO level and a decrease of arginase activity in the L-arginine-NO pathway. Furthermore, poor sperm quality may be due to excessive production of NO under psychological stress. In the light of these results, we suggest that the arginine-NO pathway, together with arginase and NO synthase, are involved in semen quality under stress conditions. Correspondence

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality

Eskiocak

Gözen

Taşkıran

et al. 2006

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…Elgun et al. (2000) reported a positive correlation between sperm motility and arginase activity in the infertile group in both seminal plasma and sperm cells.…”

Section: Discussionmentioning

confidence: 95%

“…This value of arginase activity in rams was found higher than that in cat testicular‐specific activity [0.012 U (mg protein) −1 ] (Mahmoud et al. , 2007), normal men [0.016 ± 0.005 U (mg protein) −1 ] and men with oligospermia [0.029 ± 0.009 U (mg protein) −1 ] (Elgun et al. , 2000).…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Relationships between seminal plasma arginase activity and spermatological parameters in rams

Gür

Kandemır

2011

Andrologia

View full text Add to dashboard Cite

This study was conducted to evaluate the correlation between seminal plasma arginase activity and spermatological parameters in rams. In this study, five fertility-proven Awassi rams were used as material. Six ejaculates were collected from each ram by an artificial vagina. Spermatological parameters (semen volume, mass activity, sperm motility and concentration and abnormal sperm rate) were immediately determined in each ejaculate. For enzyme assay, the semen samples were centrifuged and stored at -20 °C for the analysis of arginase activity. The average seminal plasma arginase activity was 0.61 ± 0.20 U (mg protein)(-1) . There was a positive correlation between arginase activity and semen volume (r = 0.412, P< 0.05), semen mass activity (r = 0.610, P < 0.01), sperm motility (r = 0.447, P < 0.05) and sperm concentration (r = 0.808, P < 0.01). However, there was a negative correlation between arginase activity and abnormal sperm rate (r = -0.424, P < 0.05). In conclusion, this study clearly suggests that there is a significant correlation between seminal plasma arginase activity and spermatological parameters. In light of these results, seminal plasma arginase activity may be a biochemical criterion for determining sperm quality besides classical semen analysis parameters in rams.

show abstract

“…(13,(24)(25)(26) It is therefore important to establish a convenient and sensitive ELISA method for measuring arginase. We therefore developed an efficient ELISA system for measuring the concentration of human liver-type arginase in both serum and culture medium.…”

Section: Resultsmentioning

confidence: 99%

Development of a Sandwich ELISA Test for Arginase Measurement Based on Monoclonal Antibodies

2001

View full text Add to dashboard Cite

Human arginase was purified from liver and two monoclonal antibodies (MAbs), HA1 and HA2, were produced by fusion of spleen cells from an arginase-immunized BALB/c mouse and the NS-1 myeloma cell line. Both MAbs were of the IgG3 subclass and contained the kappa light chain. HA1 inhibited arginase activity, suggesting that it binds to the arginase catalytic site. HA1 and a horseradish peroxidase-conjugated polyclonal rabbit anti-human arginase antibody were used to develop a sandwich enzyme-linked immunoadsorbent assay (ELISA) for the quantification of human arginase, which can be used in the 1 to 300 ng/mL range. Because of its sensitivity and specificity, this MAb can be successfully applied to the ELISA quantification of arginase in serum and culture supernatants.

show abstract

Seminal arginase activity in infertility

Cited by 18 publications

References 13 publications

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality

Relationships between seminal plasma arginase activity and spermatological parameters in rams

Development of a Sandwich ELISA Test for Arginase Measurement Based on Monoclonal Antibodies

Contact Info

Product

Resources

About