Seminal leukocytes and clinical outcomes with donor sperm insemination

Barraud-Lange, Virginie; Pont, Jean-Christophe; Pocate, Khaled; Kunstmann, J.-M.; Chalas-Boissonas, Céline; Ducot, B.; Wolf, Jean Philippe

doi:10.1016/j.fertnstert.2011.08.025

Cited by 13 publications

(13 citation statements)

References 11 publications

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“…Although lower fertilisation and cleavage rates have been reported in the presence of leukocytospermia [15,50], a more recent investigation reported that the ICSI outcomes were impaired when seminal samples associated with this condition were employed, even though there was a trend for an association with male infertility [16,17]. Furthermore, it was demonstrated that fertile semen donors could also have leukocytospermia (≥10 6 /mL) [51]. Another study showed a negative effect of ROS on conventional IVF cycles, which was not observed for ICSI cycles [52].…”

Section: Discussionmentioning

confidence: 99%

The influence of leukocytospermia on the outcomes of assisted reproductive technology

Cavagna

Oliveira

Petersen

et al. 2012

Reprod Biol Endocrinol

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BackgroundIt is not well established whether the increased number of leukocytes in the seminal fluid impairs the outcomes of assisted reproductive technology (ART). This investigation analysed the outcomes of the intracytoplasmic sperm injection (ICSI) and intracytoplasmic morphologically selected sperm injection (IMSI) cycles in couples in which the male partner exhibited leukocytospermia.MethodsA total of 100 cycles in 100 couples were included in this study. For the ICSI or IMSI procedures, the patients were divided into two groups according to the presence or absence of leukocytospermia and then matched by (female) age: ICSI: Group I (n = 25): Leukocytospermia - semen samples with a leukocyte count of greater than or equal to 1 × 10(6)/mL; and Group II (n = 25): Non-leukocytospermia - semen samples with a leukocyte count < 1 × 10(6)/mL. IMSI: Group I (n = 25): Leukocytospermia; and Group II (n = 25): Non-leukocytospermia.The endpoints included the rates of fertilisation, implantation, clinical pregnancy, miscarriage, ongoing pregnancy and live birth. Student’s t-tests, Mann–Whitney tests and Chi-square tests were performed, and P < 0.05 was considered significant.ResultsThe data from the ICSI groups showed that leukocytospermia did not have a negative influence on the rates of fertilisation (Group I: 57.9+/−30.2%, Group II: 61.9+/−27.7%; P = 0.74), implantation (Group I: 12.3%; Group II: 13.5%; P = 0.93), clinical pregnancy (Group I: 24%; Group II: 24%; P = 1.0), miscarriage (Group I: 0, Group II: 0), ongoing pregnancy (Group I: 24%; Group II: 24%; P = 1.0), or live births (Group I: 24%; Group II: 24%; P = 1.0). Similarly, the data from the IMSI groups also showed that the leukocytospermia did not have a negative influence on the rates of fertilisation (Group I: 67.6+/−24.6%, Group II: 59.5+/−28.1%; P = 0.36), implantation (Group I: 17.5%; Group II: 16.7%; P = 0.90), clinical pregnancy (Group I: 28%; Group II: 24%; P = 1.0), miscarriage (Group I: 14.3%; Group II: 0; P = 0.33), ongoing pregnancy (Group I: 24%; Group II: 24%; P = 1.0), or live births (Group I: 24%, 6/25; Group II: 24%, 6/25; P = 1.0).ConclusionsThe results indicate that the leukocytospermia may not have a negative effect on the outcomes of ICSI or IMSI cycles. Nevertheless, it seems that it is necessary to more precisely determine the effects, if any, of seminal leukocytes on fertilisation and implantation processes. Such efforts will help to establish a more reliable leukocyte threshold, which could eventually demonstrate whether there is a negative influence on the ART procedures.

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Section: Discussionmentioning

confidence: 99%

The influence of leukocytospermia on the outcomes of assisted reproductive technology

Cavagna

Oliveira

Petersen

et al. 2012

Reprod Biol Endocrinol

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“…[2] Should leukocytospermia be treated in infertile men? The first question is difficult to answer because fertile men do not come to the clinic, but a study of fertile donors' sperm suggested that they may have leukocytospermia too, with the same consequences of increased early pregnancy loss in recipients (38). To answer the second question, a therapeutic trial involving a large number of patients is necessary.…”

Section: Discussionmentioning

confidence: 99%

Seminal leukocytes are Good Samaritans for spermatozoa

Barraud-Lange

Pont

Ziyyat

et al. 2011

Fertility and Sterility

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“…Moreover, according to some authors, leukocytospermia represents an essential or additional risk factor that needs to be treated if sperm quality is to be improved [61,62]. On the other hand, the observed alterations of standard sperm parameters accompanying leukocytospermia have not always been associated with a decreased fertilizing ability of the sperm, especially in assisted conception procedures [63,64]. These data are indirectly in agreement with the results that we obtained in an in vitro model of semen bacterial infection, indicating the detrimental effect of leukocytes alone on sperm motility and the hypoosmotic swelling (HOS) test.…”

Section: Coli Staphylococcus (S) Aureus Enterococcus (E) Faecalismentioning

confidence: 99%

Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen

Frączek

Kurpisz

2015

Folia Histochem Cytobiol.

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The invasion of the male reproductive tract by microorganisms, and its subsequent consequences for sperm fertilizing potential, has been intensely discussed. The role of the bacteria that are responsible for the colonization and contamination of the male urogenital tract, rather than its infection, in diminished sperm parameters raises the most controversy. There are numerous premises suggesting that bacterial semen infection is associated with male infertility. However, the molecular mechanism by which the fertility is affected is complex and multifactorial, and still presents a puzzle. Some authors have suggested that direct interactions between bacteria and human spermatozoa facilitate sperm immobilization, affect sperm morphology, and thus weaken the ability of sperm to fertilize. On the other hand, the massive infiltration of activated leukocytes into the inflammatory site may be associated with impairment of sperm fertilizing potential, due to oxidative, apoptotic, and immune processes. This review presents current research trends and aims to summarize the present knowledge of semen inflammation and causative bacterial agents in the male urogenital tract, with its consequence on seminological parameters, and male fertility status.

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Seminal leukocytes and clinical outcomes with donor sperm insemination

Cited by 13 publications

References 11 publications

The influence of leukocytospermia on the outcomes of assisted reproductive technology

The influence of leukocytospermia on the outcomes of assisted reproductive technology

Seminal leukocytes are Good Samaritans for spermatozoa

Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen

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