Seminal plasma Alters surface Glycoprofile of dromedary camel cryopreserved epididymal spermatozoa

Desantis, Salvatore; Lacalandra, Giovanni Michele; Batista, M.; Amann, O. V.; Antonelli, Dario; Monaco, Davide

doi:10.1016/j.theriogenology.2021.03.008

Cited by 7 publications

(5 citation statements)

References 56 publications

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“… 32 It is well known that the sperm glycocalyx is rich in galactose residues and is modified during epididymal transit; 49 in addition, recently, Desantis et al demonstrated that the seminal plasma affects the surface glycoprofile of animal spermatozoa. 50 From these observations, we can hypothesize that inflammation caused by varicocele and genitourinary infections can be a trigger for omentin-1/ITLN1 secretion and, potentially, for overexpression of galactose residues in the sperm glycocalyx that could explain the diffuse omentin-1/ITLN1 signal detectable in spermatozoa of patients with these pathologies.…”

Section: Discussionmentioning

confidence: 90%

Seminal Levels of Omentin-1/ITLN1 in Inflammatory Conditions Related to Male Infertility and Localization in Spermatozoa and Tissues of Male Reproductive System

Moretti¹,

Signorini²,

Noto³

et al. 2022

JIR

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Purpose Omentin-1/intelectin (ITLN)1 is an adipocytokine with both anti-inflammatory and anti-oxidative stress properties, and little is known about its role in male reproduction. This study was aimed at exploring the relationships among omentin-1/ITLN1, semen parameters and F 2 -isoprostanes (F 2 -IsoPs), a maker of oxidative stress, in groups of patients affected by different pathologies. In addition, omentin-1/ITLN1 immunolocalization was assessed in ejaculated spermatozoa and in tissues of male reproductive system. Patients and Methods Semen samples of infertile patients with varicocele (n = 27), genitourinary infections (n = 17), idiopathic infertility (n = 15) and fertile men (n = 21) were analyzed following WHO guidelines, and seminal plasma were used to determine omentin-1/ITLN1 by ELISA and F 2 -IsoP levels by gas chromatography/negative-ion chemical ionization tandem mass spectrometry. Omentin-1/ITLN1 was localized in human sperm and in the tissue of male reproductive system. Results Considering all participants, F 2 -IsoP and omentin-1/ITLN1 levels were positively correlated (p = 0.000), and both these indices were negatively correlated with sperm parameters. Infertile patients showed lower sperm parameters than fertile ones; varicocele and infection groups had significantly increased levels of F 2 -IsoPs (both p = 0.000) and omentin-1/ITLN1 (p = 0.000 and p = 0.001, respectively). Omentin-1/ITLN1 signal was located as a spot in the connecting piece (in 43.5% of cases midpiece was also labeled) of sperm from fertile men and in cytoplasmic residue and in the entire tail in sperm of patients with varicocele and genitourinary infections. A focal omentin-1/ITLN1 immunolabelling was evident in the basal area of epididymal tubule, and a diffuse signal was present in the seminal vesicle epithelium. Conclusion Semen omentin-1/ITLN1 originates from seminal vesicles, its levels increase in inflammatory conditions and are negatively correlated with sperm parameters. For this reason, a sort of protective role of omentin-1/ITLN1 can be postulated, as this adipokine shows anti-inflammatory properties also in many other biological systems.

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Section: Discussionmentioning

confidence: 90%

Seminal Levels of Omentin-1/ITLN1 in Inflammatory Conditions Related to Male Infertility and Localization in Spermatozoa and Tissues of Male Reproductive System

Moretti¹,

Signorini²,

Noto³

et al. 2022

JIR

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“…In order to better understand this aspect and aspects related to the SP, samples of Exp 1 and Exp 3 were fixed, smeared, and stored at 4 • C for additional studies to be performed on sperms' lectins and glycocalyx. Those studies will also include the evaluation of the PNA reactive sperms (acrosomes) [24], but due to the high number of lectins to be evaluated, this analysis is expected to be completed at a later date and thus will be reported and discussed in another study.…”

Section: Discussionmentioning

confidence: 99%

“…Adult (>5 years of age) dromedary camel bull testis were retrieved at the local slaughterhouse, kept at room temperature, and processed within two hours of collection. Epididymal Spermatoza were recovered by retrograde flushing with TCF, according to a previously described technique [9,24]. Samples were kept in a water bath at 37 • C and evaluated for concentration (spectrophotometer), morphology (Eosin/Nigrosin staining [25]), and motility.…”

Section: Epididymal Semen Collection and Evaluationmentioning

confidence: 99%

The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull Spermatozoa

Monaco,

Lacalandra,

Ansar

et al. 2023

Animals

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In order to contribute to the development of semen processing procedures in camelids, the aims of the present study were to evaluate (i) the effect of 35% seminal plasma incubation on dromedary camel epididymal sperm motility and kinematic parameters, (ii) the effects of centrifugation, with cushion fluid and enzymatic reduction of viscosity (Papain + E64) during ejaculate processing, on the motility and kinematic parameters of dromedary camel ejaculates. The incubation with seminal plasma significantly reduced the percentage of progressively motile spermatozoa as well as the proportion of medium progressive spermatozoa whilst increasing the percentage of non-progressive spermatozoa. The centrifugation procedure improved the sperms’ kinematic parameters, and the highest values were observed for samples centrifugated with cushion fluid. The samples treated with Papain + E64 showed a significant increase in both total and medium progressive spermatozoa, along with a reduction of non-progressive spermatozoa (p < 0.05). The results of this investigation show that a simple, cheap, and effective procedure, such as cushioned centrifugation, could improve the motility patterns of dromedary camel spermatozoa; in combination with enzymatic reduction of viscosity, this method leads to the best results in terms of recovery rates and sperms’ kinematic parameters.

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“…Camel erythrocytes carry high amounts of sialic acid [342]. No information is available on the expression patterns of α2,6-SA receptors in the camel respiratory tract, although both α2,3-SA and α2,6-SA have been detected in camel serum samples [343], the oviduct epithelium [344], and spermatozoa [345].…”

Section: Camelsmentioning

confidence: 99%

Zoonotic Animal Influenza Virus and Potential Mixing Vessel Hosts

Abdelwhab

Mettenleiter

2023

Viruses

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Influenza viruses belong to the family Orthomyxoviridae with a negative-sense, single-stranded segmented RNA genome. They infect a wide range of animals, including humans. From 1918 to 2009, there were four influenza pandemics, which caused millions of casualties. Frequent spillover of animal influenza viruses to humans with or without intermediate hosts poses a serious zoonotic and pandemic threat. The current SARS-CoV-2 pandemic overshadowed the high risk raised by animal influenza viruses, but highlighted the role of wildlife as a reservoir for pandemic viruses. In this review, we summarize the occurrence of animal influenza virus in humans and describe potential mixing vessel or intermediate hosts for zoonotic influenza viruses. While several animal influenza viruses possess a high zoonotic risk (e.g., avian and swine influenza viruses), others are of low to negligible zoonotic potential (e.g., equine, canine, bat and bovine influenza viruses). Transmission can occur directly from animals, particularly poultry and swine, to humans or through reassortant viruses in “mixing vessel” hosts. To date, there are less than 3000 confirmed human infections with avian-origin viruses and less than 7000 subclinical infections documented. Likewise, only a few hundreds of confirmed human cases caused by swine influenza viruses have been reported. Pigs are the historic mixing vessel host for the generation of zoonotic influenza viruses due to the expression of both avian-type and human-type receptors. Nevertheless, there are a number of hosts which carry both types of receptors and can act as a potential mixing vessel host. High vigilance is warranted to prevent the next pandemic caused by animal influenza viruses.

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Seminal plasma Alters surface Glycoprofile of dromedary camel cryopreserved epididymal spermatozoa

Cited by 7 publications

References 56 publications

Seminal Levels of Omentin-1/ITLN1 in Inflammatory Conditions Related to Male Infertility and Localization in Spermatozoa and Tissues of Male Reproductive System

Seminal Levels of Omentin-1/ITLN1 in Inflammatory Conditions Related to Male Infertility and Localization in Spermatozoa and Tissues of Male Reproductive System

The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull Spermatozoa

Zoonotic Animal Influenza Virus and Potential Mixing Vessel Hosts

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