Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing1,2

Abstract

Boar spermatozoa arising from the sperm-rich ejaculate fraction are reported to have a more stable plasma membrane and are more resistant to cold shock and premature acrosome reaction than spermatozoa from the whole ejaculate. Furthermore, seminal plasma (SP) can increase the cryotolerance of boar spermatozoa, and in other domestic species, it has the ability to reverse cryopreservation damage. This study aimed to evaluate the effects of boar SP arising from the whole sperm-rich ejaculate fraction (SP-SRF) on the integrity, stability, and peroxidation of sperm membranes after thawing. Each ejaculate ( = 24) was divided among 4 treatments: control (CT), centrifuged and suspended in autologous SP-SRF (CS), centrifuged with withdrawn SP-SRF (CW), and post-thawed SP arising from the whole sperm-rich fraction addition to CW (CWSP). After thawing, all treatments were incubated for 5, 60, and 120 min and were analyzed for membrane integrity, fluidity, and peroxidation by flow cytometer. The absence of SP-SRF increased the lipid disorder ( < 0.05) but had no effect on lipid peroxidation ( > 0.05) or membrane integrity ( > 0.05). However, the increase in lipid disorder by withdrawal of SP-SRF was reversed by SP-SRF addition ( < 0.05) to the post-thawing medium, whereas plasma and acrosomal membrane integrity ( > 0.05) and lipid peroxidation ( > 0.05) were unchanged. In conclusion, despite the centrifugation effects, the addition of SP arising from the whole sperm-rich fraction to post-thawed boar semen decreased sperm lipid disorder without an influence of the sperm membrane integrity and peroxidation.

Cryopreservation of boar semen in 0.5mL straws at low spermatozoa concentration is better than high concentration to maintain sperm viability

“…The freezing rate was -20°C/min from 5 to -120°C. Subsequently, the straws were immersed in liquid nitrogen at -196°C and stored in goblets within cryogenic tanks (Torres et al 2016a). Two straws per ejaculate and treatment were thawed in a water bath at 37°C for 30 seconds and subsequently pooled.…”

Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability

“…Studies indicate that BSPs protect ram spermatozoa subjected to cryopreservation 13 and empirically relate to fertility of dairy bulls evaluated both in vitro 14 and in vivo 15 . Treatment of sperm with diluted seminal plasma and sperm-rich fraction of seminal plasma is beneficial to frozen-thawed sperm from boars 16 , 17 and cryotolerance of boar sperm can be estimated based on phosphorylation patterns of HSP70 18 , indicating that seminal components are important for viability of cryopreserved sperm. In addition to direct effects on sperm physiology, seminal proteins also appear to influence post-fertilization events as well.…”

Prooxidant-antioxidant homeostasis in boars depending on the types of higher nervous activity

“…Це насамперед полягає у відсутності активності СОД та кількості вітаміну А і вітаміну Е у першій та четвертій фракціях їх еякулятів. Різну біохімічну і фізіологічну повноцінність різних фракцій еякулятів також відмічено у дослідженнях (Torres et al, 2016;Ratchamak, 2019). Проте виявлено, що найоптимальніше формується спермопродукція в особин сильного врівноваженого жвавого та спокійного типів ВНД (Stoianovskyi et al, 2020).…”