Seminal plasma PSA in spinal cord injured men: a preliminary report

Brasso, Klaus; Sønksen, Jens; Sommer, Peter; Ødum, Lars; Biering‐Sørensen, Fin; Iversen, Peter; Kristensen, Jørgen Kvist

doi:10.1038/sj.sc.3100705

Cited by 17 publications

(12 citation statements)

References 17 publications

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“…Moreover, ACPP, a tyrosine phosphatase that dephosphorylates large numbers of substrates and which has been widely used a biomarker for prostate cancer (51), was observed to be around sevenfold more abundant in the SP of controls. Thus, our proteomic results strongly support what other studies have only hypothesized (12,52), i.e. that a prostatic dysfunction could somehow be involved in SCI related infertility.…”

Section: Qualitative Map Of the Human Seminal Plasma Proteome-supporting

confidence: 81%

“…The neurogenic stimulation of the male accessory sexual glands including the prostate arises from sympathetic nerves from the thoracolumbar spinal cord (T11-L2) and from parasympathetic nerves from the sacral spinal cord (S1-S4) (54). Brasso et al reported that SP concentrations of KLK3 were significantly lower in SCI patients with lesions below T7 compared with patients with lesions at or above this level (52). Studies have shown that the prostate gland is smaller in men with SCI compared with healthy, noninjured men, and that this effect may be because of a prolonged central (rather than peripheral) desinnervation of the prostate gland (55).…”

Section: Qualitative Map Of the Human Seminal Plasma Proteome-mentioning

confidence: 99%

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Towards Understanding Male Infertility After Spinal Cord Injury Using Quantitative Proteomics

Silva

Meng

Helm

et al. 2016

Molecular & Cellular Proteomics

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The study of male infertility after spinal cord injury (SCI) has enhanced the understanding of seminal plasma (SP) as an important regulator of spermatozoa function. However, the most important factors leading to the diminished sperm motility and viability observed in semen of men with SCI remained unknown. Thus, to explore SP related molecular mechanisms underlying infertility after SCI, we used mass spectrometry-based quantitative proteomics to compare SP retrieved from SCI patients to normal controls. As a result, we present an in-depth characterization of the human SP proteome, identifying ϳ2,800 individual proteins, and describe, in detail, the differential proteome observed in SCI. Our analysis demonstrates that a hyper-activation of the immune system may influence some seminal processes, which likely are not triggered by microbial infection. Moreover, we show evidence of an important prostate gland functional failure, i.e. diminished abundance of metabolic enzymes related to ATP turnover and those secreted via prostasomes. Further we identify the main outcome related to this fact and that it is intrinsically linked to the low sperm motility in SCI. Together, our data highlights the molecular pathways hindering fertility in SCI and shed new light on other causes of male infertility. Molecular & Cellular Proteomics 15: 10.1074/mcp.M115.052175, 1424-1434, 2016.For many years, seminal plasma (SP) 1 , the liquid component of semen, was believed to have a single and simple physiological significance as the carrier of spermatozoa through both male and female reproductive tracts. It was around 50 years ago when the compositional complexity of this fluid started to be investigated, demonstrating that SP not only aids in cellular transport but also provides energy and metabolic support to the transiting spermatozoa (1, 2). Today, growing evidence indicates that SP plays a role far beyond what was once envisioned, including acting as an essential regulator of spermatozoa function contributing to (enabling/ hindering) the cellular ability of fertilization (3). Composed of secretions derived from the testis, epididymis and male accessory glands (prostate, seminal vesicles and bulbourethral glands), SP is a mixture of sugars, inorganic ions, organic salts, (phospho)lipids and proteins (4). Such a heterogeneous composition emphasizes the complex biochemical cascades triggered within SP during, and immediately after, ejaculation and defines the beneficial and/or detrimental nature of SP in the overall reproductive process (5). From a clinical point of view, studies have confirmed the participation of SP in the etiology of male infertility. By studying semen of men with spinal cord injury (SCI), who become infertile after a traumatic injury and often present with an unusual seminal profile characterized by normal sperm concentration but extremely impaired sperm motility and viability, Brackett et al. demonstrated how SP can impair sperm function leading to infertility (6). Specifically, the authors mixed SP obtained from ...

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Section: Qualitative Map Of the Human Seminal Plasma Proteome-supporting

confidence: 81%

Section: Qualitative Map Of the Human Seminal Plasma Proteome-mentioning

confidence: 99%

Towards Understanding Male Infertility After Spinal Cord Injury Using Quantitative Proteomics

Silva

Meng

Helm

et al. 2016

Molecular & Cellular Proteomics

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“…Seminal plasma PSA concentration is approximately 10 5 -10 6 times higher than serum PSA concentration and the majority of PSA in seminal plasma is present in the catalytically active single-chain form. 23 Brasso et al 24 evaluated the seminal plasma PSA concentrations in 16 patients with SCI compared to 20 normal controls. The concentration of PSA in seminal plasma depended on the level of SCI, with significantly lower concentrations in patients with lesions below T7 compared to both normal age-matched controls and patients with lesions at or above T7.…”

Section: Seminal Plasma Prostate-specific Antigen Levels In Men With mentioning

confidence: 99%

“…The results are shown in Table 3. 18,20,24 Prostate cancer diagnosis in men with spinal cord injury It has been theorized that central denervation of an organ is protective against malignancy and changes in Prostate activity and prostate cancer SCI HB Shim et al the hypothalamic-pituitary-gonadal axis but no persuasive data are available. Historically, since SCI patients rarely survived into the years during which prostate cancer becomes a significant medical issue, they were not screened and death from prostate cancer in patients with SCI was rare.…”

Section: Seminal Plasma Prostate-specific Antigen Levels In Men With mentioning

confidence: 99%

Prostate activity and prostate cancer in spinal cord injury

Shim

Jung

Lee

et al. 2006

Prostate Cancer Prostatic Dis

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In addition to androgen, autonomic nerves may be involved in prostatic function. As patients with spinal cord injury (SCI) have impaired innervation of the prostate, the prostate volume and prostatespecific antigen (PSA) level in patients with SCI may be different from those of healthy men. Experiments in rats with SCI indicate that neurogenic factors play an important role in prostate growth and function but the same phenomena may not occur in men with SCI because the current animal models differ from clinical results in humans in several respects. Although many of studies indicate the importance of intact peripheral innervation on the secretory function of the prostate, the effect of more central denervation such as occurs in complete SCI at the cervical, thoracic, or lumbar levels on prostatic function is unclear. In addition, the impact of central nervous system injury on prostatic secretory activity, and consequently on serum PSA levels, is also not clear. Furthermore, the impact of hormonal changes on prostate cancer development and prognosis observed in patients with SCI may also be affected by the level of injury and patient age at the time of injury, which have not been studied. In this article, we review prostate activity and prostate cancer in SCI and discuss how they may relate to neurogenic factors.

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“…However, most authors agree that the best motility is obtained when semen is collected by VE than by electroejaculation. 15 Brasso et al 16 have studied seminal PSA using either VE or electroejaculation for collecting semen. They have not found statistical difference between the two methods.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of seminal zinc levels by atomic absorption in men with spinal cord injury

et al. 2010

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Study design: A case-control study evaluating seminal zinc level in spinal cord injury (SCI) patients. Objectives: Patients with SCI have neurological prostate dysfunction. There are only some indications in the literature that seminal zinc level may be lower in these patients. Seminal zinc is mainly produced by the prostate and, therefore, can be considered to be a marker of prostate function. The objective of the present study was to determine whether SCI can induce changes in seminal zinc levels and to compare the results with those obtained for normal men (controls). Setting: The study was carried out in Brazil. Methods: A total of 24 men with SCI (mean age ± s.d. 36.25 ± 10.24 years) and 24 controls (mean age±s.d. 36.50±10.31 years) were studied. Blood and semen were collected after 3 days of abstinence from ejaculation. Semen was left at room temperature for 15 min, stored in liquid nitrogen, and lyophilized. Seminal zinc was determined by atomic absorption. Blood was stored at a controlled temperature of -70 to À79 1C and later used for the determination of testosterone, prolactin and total prostate-specific antigen using an AxSYM apparatus and Abbott reagents. Results: Mean seminal zinc concentration was 85.20 mg l À1 for the patients, a lower value than that obtained for the controls (147.16 mg l À1 ) (P ¼ 0.0035). Conclusion: Patients with SCI have a significant reduction of seminal zinc.

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Seminal plasma PSA in spinal cord injured men: a preliminary report

Cited by 17 publications

References 17 publications

Towards Understanding Male Infertility After Spinal Cord Injury Using Quantitative Proteomics

Towards Understanding Male Infertility After Spinal Cord Injury Using Quantitative Proteomics

Prostate activity and prostate cancer in spinal cord injury

Evaluation of seminal zinc levels by atomic absorption in men with spinal cord injury

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