Sensibilidad de bacterias procedentes de pasifloras a antibióticos y productos cúpricos

Farfán, Lina M.; Benítez, Solange V.; Hoyos-Carvajal, Lilliana

doi:10.17584/rcch.2014v8i1.2797

Cited by 6 publications

(4 citation statements)

References 20 publications

(41 reference statements)

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“…Furthermore, all isolates showed oxytetracycline susceptibility to Gen + Ox and 47% were completely inhibited by Gen. Oxytetracycline resistance by isolates differs with that reported by Farfán, Benítez & Carvajal (2014), who found Xanthomonas sp. resistant to Ox at ≤ 25 µg/mL.…”

Section: Discussioncontrasting

confidence: 62%

“…For minimum inhibitory concentration (MIC) analysis, antimicrobial dilutions were prepared at 4, 8, 16, 32, 64, 128, 256, 512, and 640 µg/mL in tryptone soy broth (TSB). Microbial resistance was defined by Xanthomonas growth on copper compounds at 200 µg/mL (Zhang et al, 2013;Roach et al, 2020), on Ox at 25 µg/mL (Farfán, Benítez & Carvajal, 2014), and on gentamicin at 25 µg/mL (Rojas, Peña & Peña-Vera, 2019). In addition, pH values from all the antimicrobial tested and their dilutions were determined.…”

Section: Pathogenicity Testmentioning

confidence: 99%

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Prevalence of Xanthomonas euvesicatoria (formally X. perforans) associated with bacterial spot severity in Capsicum annuum crops in South Central Chihuahua, Mexico

Hernández-Huerta

Tamez‐Guerra

Gómez-Flores

et al. 2021

PeerJ

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Background Xanthomonas spp. causes bacterial spot disease, which reduces quality and yield of pepper crops in Mexico. Identification of phytopathogen species is necessary to implement more effective control strategies. Objective The aim of this study was to isolate and identify infecting Xanthomonas species in South Central Chihuahua pepper-producing areas. Methods Diseased plants were collected from 30 cultivation lots and bacteria were isolated from damaged tissues. Potential causative agents were isolated, identified, and characterized by biochemical and molecular analysis. Pathogenicity tests from each isolate were then performed on 30-d-old pepper seedlings, exposing five leaves to 10 µL of 1 × 108 CFU/mL bacterial suspensions of each isolate, using sterile distilled water as a control. Disease severity was determined after 10 d by calculating leaf damage percentage. Furthermore, we evaluated the susceptibility of the highest bacterial spot severity-causing isolates (13 isolates) to copper sulphate (CuS), copper gluconate (CuG), copper oxychloride + oxytetracycline hydrochloride (Cu + Ox), gentamicin + oxytetracycline hydrochloride (Gen + Ox), and gentamicin sulphate (GenS). Copper-resistance genes (copLAB) were detected by PCR analysis among isolates. Results Thirty-seven foliage isolates were identified as Xanthomonas euvesicatoria (14%), which were associated with bacterial spot disease in jalapeño pepper. Tested Xanthomonas isolates were resistant to Cu-based compounds, but susceptible to Cu + Ox. All isolates were susceptible to Gen + Ox and GenS. CopLAB genes were detected in all but one strain. Conclusions X. euvesicatoria (formally X. perforans) may be considered as an emerging pathogen of bacterial spot pepper in Mexico. Among disease management strategies, alternatives to copper should be taken into consideration.

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Section: Discussioncontrasting

confidence: 62%

Section: Pathogenicity Testmentioning

confidence: 99%

Prevalence of Xanthomonas euvesicatoria (formally X. perforans) associated with bacterial spot severity in Capsicum annuum crops in South Central Chihuahua, Mexico

Hernández-Huerta

Tamez‐Guerra

Gómez-Flores

et al. 2021

PeerJ

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“…There are few recent publications relating to the use of antibiotics on crops and those that do exist often cite review papers that refer to earlier sources. Literature that describes contemporary use of antibiotics on plants is usually confined to that of extension literature (https ://exten sion.psu.edu/pear-disea se-fire-bligh t), or reporting concerns over the development of antibiotic resistance in populations of plant pathogens (Sundin and Wang 2018;Farfán et al 2014). Exceptions to this are two recent papers from China that provide some insights into antibiotics being recommended by extension services in the country (Zhang et al 2017;Wan et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Antibiotic use on crops in low and middle-income countries based on recommendations made by agricultural advisors

2020

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Background: Microbial resistance to medically important antibiotics is of international concern. There is considerable attention paid to the medical and veterinary use of antibiotics but there is a paucity of data on their use in global crop production. The only well documented use of antibiotics on crops is that on top fruit in the USA. Due to the absence of other data it is generally assumed that this use comprises the bulk of antibiotics applied to plants. The goal of this study was to investigate the scale and diversity of antibiotics being recommended for managing crop health problems in LMICs and the crops and types of problems for which they are selected. Methods: Plantwise is an international program which assists with the provision of agronomic advice to smallholder farmers in LMICs. Recommendations relating to the management of crop problems are stored in an international database, comprising over 400,000 records collected over 8 years. The extent of antibiotic use in crop production when grouped by the WHO regions was analysed using descriptive statistics. Results: Within our data all WHO regions of the world are using antibiotics on crop plants with the exception of Africa (no data for Europe) and the main crop on which they are used is rice. In some years, and in one region, nearly 10% of the management recommendations for rice contained an antibiotic. Eleven antibiotics are being recommended on crops (often blended together) and there is considerable regional variation as to where they are used. The problems against which antibiotics are recommended are extremely varied and we speculate that they are often used as a prophylactic spray to prevent, or control, low levels of bacterial disease. Conclusions: The data reveals that antibiotics are being recommended far more frequently and on a much greater variety of crops than previously thought. Relative to medical and veterinary use the quantities used globally are comparatively small, but this niche does provide some unique avenues by which resistance could develop in human pathogens. Results presented here have implications for those wanting to limit the spread of antibiotic resistance.

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“…Among these microorganisms Xanthomonas bacteria and Fusarium fungus are frequently involved (Fernández Barbosa and Suarez Meza, 2009;Benítez et al 2011). Additionally, prolonged cultivation, the use of antibiotics, and poor agricultural practices have led to the development of resistance in some of these phytopathogens (Farfán et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens

Núñez-Rangel,

Rey-Suárez,

Serna

et al. 2023

Rev. Colomb. Cienc. Hortic

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The most important pathogens causing high damage to fruit and vegetable crops are bacteria and fungi; among them Xanthomonas and Fusarium respectively are in the phytopathogens responsible of diseases in those crops. In Colombia, the crop of Passiflora edulis f. edulis occupies a position preferential within international market. However different pathologies affect their production, one of them is the infectious diseases and are caused by microorganisms as Xanthomonas and Fusarium. The control includes application of pesticides or antibiotics, but to some of them the microorganisms have develop resistance is widespread, and others are restricted associated to the impact on the environment and effects in animal and human healthy. Then find other alternatives is necessary, in this work the snake venoms of the vipers Bothrops asper and Porthidium nasutum were evaluated in ability to inhibit growth of these microorganism isolated of purple passion fruit crops with typical symptoms of diseases associated with infections by microorganisms. For these antimicrobial assays were performed in agar culture and broth microdilution. The results showed growth inhibition of 80% to B. asper against Xanthomonas axonopodis and 70% Xanthomonas perforans. The minimum inhibitory concentration was 31,2 µg mL-1 to B. asper and 500 µg mL-1 to P. nasutum to both bacteria. Nevertheless, the activity against F. oxysporum was low. The isolation of compounds responsible of this activity will contribute to the development of new molecules for antimicrobial agents against phytopathogens affecting purple passion fruit crops.

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Sensibilidad de bacterias procedentes de pasifloras a antibióticos y productos cúpricos

Cited by 6 publications

References 20 publications

Prevalence of Xanthomonas euvesicatoria (formally X. perforans) associated with bacterial spot severity in Capsicum annuum crops in South Central Chihuahua, Mexico

Prevalence of Xanthomonas euvesicatoria (formally X. perforans) associated with bacterial spot severity in Capsicum annuum crops in South Central Chihuahua, Mexico

Antibiotic use on crops in low and middle-income countries based on recommendations made by agricultural advisors

Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens

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