Sensing dynamic cytoplasm refractive index changes of adherent cells with quantitative phase microscopy using incorporated microspheres as optical probes

Przibilla, Sabine; Dartmann, Sebastian; Vollmer, Angelika; Ketelhut, Steffi; Greve, Burkhard; Bally, Gert von; Kemper, Björn

doi:10.1117/1.jbo.17.9.097001

Cited by 34 publications

(40 citation statements)

References 54 publications

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“…There have been some significant advances recently in non-invasive, label-free imaging1. Examples of these include digital holographic microscopy234567 and ptychography8910.…”

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confidence: 99%

“…Examples of these include digital holographic microscopy (DHM)234567 and the transport-of-intensity equation (TIE)21. For example, DHM has been used to provide quantitative phase images of live cells2 in vitro, to detect cell division in endothelial cells3, to determine the refractive index using incorporated microspheres5 and to detect early cell death based on cell volume changes6. In DHM a reference beam is interfered with a beam scattered by a specimen such that the intensity of the resulting fringe pattern encodes information about the sample's phase-altering properties.…”

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confidence: 99%

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Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information

Marrison

Räty

Marriott

et al. 2013

Sci Rep

159

116

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Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their in vivo state. We report on the biological application of a, label-free, high contrast microscopy technique known as ptychography, in which the image producing step is transferred from the microscope lens to a high-speed phase retrieval algorithm. We demonstrate that this technology is appropriate for label-free imaging of adherent cells and is particularly suitable for reporting cellular changes such as mitosis, apoptosis and cell differentiation. The high contrast, artefact-free, focus-free information rich images allow dividing cells to be distinguished from non-dividing cells by a greater than two-fold increase in cell contrast, and we demonstrate this technique is suitable for downstream automated cell segmentation and analysis.

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“…There have been some significant advances recently in non-invasive, label-free imaging1. Examples of these include digital holographic microscopy234567 and ptychography8910.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information

Marrison

Räty

Marriott

et al. 2013

Sci Rep

159

116

View full text Add to dashboard Cite

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“…Furthermore, non-invasive DHM measurement of dry mass can be performed repetitively from living cells and may be continued over a longer period of time as we demonstrate [21]. In addition, the DHM-based approach for determination of the dry mass is independent of the intracellular water content which may be influenced by the osmolality of the cell culture medium or the tested agent [50]. Importantly, simultaneous determination of cellular dry mass changes during wound healing in vitro is not provided by traditional approaches.…”

Section: Discussionmentioning

confidence: 90%

Quantitative Stain-Free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy

et al. 2014

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Impaired epithelial wound healing has significant pathophysiological implications in several conditions including gastrointestinal ulcers, anastomotic leakage and venous or diabetic skin ulcers. Promising drug candidates for accelerating wound closure are commonly evaluated in in vitro wound assays. However, staining procedures and discontinuous monitoring are major drawbacks hampering accurate assessment of wound assays. We therefore investigated digital holographic microscopy (DHM) to appropriately monitor wound healing in vitro and secondly, to provide multimodal quantitative information on morphological and functional cell alterations as well as on motility changes upon cytokine stimulation. Wound closure as reflected by proliferation and migration of Caco-2 cells in wound healing assays was studied and assessed in time-lapse series for 40 h in the presence of stimulating epidermal growth factor (EGF) and inhibiting mitomycin c. Therefore, digital holograms were recorded continuously every thirty minutes. Morphological changes including cell thickness, dry mass and tissue density were analyzed by data from quantitative digital holographic phase microscopy. Stimulation of Caco-2 cells with EGF or mitomycin c resulted in significant morphological changes during wound healing compared to control cells. In conclusion, DHM allows accurate, stain-free and continuous multimodal quantitative monitoring of wound healing in vitro and could be a promising new technique for assessment of wound healing.

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“…This value of the refractive index corresponds to one of the cell cytoplasm (Przibilla et al 2012). The wave is polarized in the direction of the x axis.…”

Section: Introductionmentioning

confidence: 98%

Energy breathing of nanoparticles

Дынич

2015

J Nanopart Res

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The paper considers the energy exchange process of the electromagnetic wave with a spherical metal nanoparticle. Based on the account of the temporal dependencies of electric and magnetic fields, the author presents an analytical dependence of the energy flow passing through the spherical surface. It is shown that the electromagnetic energy, localized in metal nanoparticles, is not a stationary value and periodically varies with time. A consequence of the energy nonstationarity is a nonradiating exit of the electromagnetic energy out of the nanoparticle. During the time equal to the period of wave oscillations, the electromagnetic energy is penetrating twice into the particle and quits it twice. The particle warms up because of the difference in the incoming and outgoing energies. Such ''energy breathing'' is presented for spherical Ag and Au nanoparticles with radii of 10 b 33 nm, respectively. Calculations were conducted for these nanoparticles embedded into the cell cytoplasm near the frequencies of their surface plasmon resonances.

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Sensing dynamic cytoplasm refractive index changes of adherent cells with quantitative phase microscopy using incorporated microspheres as optical probes

Cited by 34 publications

References 54 publications

Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information

Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information

Quantitative Stain-Free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy

Energy breathing of nanoparticles

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