Sensing parasites: Proteomic and advanced bio-detection alternatives

Sánchez-Ovejero, Carlos; Benito‐Lopez, Fernando; Díez, Paula; Casulli, Adriano; Siles-Lucas, Mar; Fuentes, Manuel; Manzano-Román, Raúl

doi:10.1016/j.jprot.2015.12.030

Cited by 24 publications

(10 citation statements)

References 157 publications

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“…Even so, these methods are not widely used. In our opinion, there is a colossal need to diagnose foodborne infections using new methodologies, such as MS and biomarkers detection, according to their extreme specificity and possible increase of the detection rate [ 242 ].…”

Section: Targeted Approach—proteomics Methods Proposed To Use For mentioning

confidence: 99%

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

Stryiński

Łopieńska–Biernat

Carrera

2020

Foods

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Foodborne parasitoses compared with bacterial and viral-caused diseases seem to be neglected, and their unrecognition is a serious issue. Parasitic diseases transmitted by food are currently becoming more common. Constantly changing eating habits, new culinary trends, and easier access to food make foodborne parasites’ transmission effortless, and the increase in the diagnosis of foodborne parasitic diseases in noted worldwide. This work presents the applications of numerous proteomic methods into the studies on foodborne parasites and their possible use in targeted diagnostics. Potential directions for the future are also provided.

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Section: Targeted Approach—proteomics Methods Proposed To Use For mentioning

confidence: 99%

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

Stryiński

Łopieńska–Biernat

Carrera

2020

Foods

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“…All this notwithstanding, PMF is arguably the leading approach to single cell diagnostic chips, as shown by many reviews over the last two decades detailing the usages of PMF diagnostic chips, also called microfluidic analytic devices (µPADs) [20,33,78,[201][202][203][204][205]. PMF has been combined with many other techniques to create a plethora of applications: from combining PMF with immunoassays [206] for Hepatitis C-tests, via on-board batteries [207] or cell phones [203] to generate field-applicable µPADs, to proteomics [208] parasite diagnostics. How established µPADs are in our everyday life is demonstrated by the usage of paper microfluidics for cheap home tests for male (and female) fertility and sperm DNA integrity [16][17][18][19][20].…”

Section: Paper Microfluidicsmentioning

confidence: 99%

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

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In the last three decades, microfluidics and its applications have been on an exponential rise, including approaches to isolate rare cells and diagnose diseases on the single-cell level. The techniques mentioned herein have already had significant impacts in our lives, from in-the-field diagnosis of disease and parasitic infections, through home fertility tests, to uncovering the interactions between SARS-CoV-2 and their host cells. This review gives an overview of the field in general and the most notable developments of the last five years, in three parts: 1. What can we detect? 2. Which detection technologies are used in which setting? 3. How do these techniques work? Finally, this review discusses potentials, shortfalls, and an outlook on future developments, especially in respect to the funding landscape and the field-application of these chips.

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“…Other types of analytes that could be studied in the exosomal space, such as lipids, might represent good biomarkers to investigate in the near future. All these findings provide an important base to continue research in parasite-derived exosomes as diagnostic targets and demonstrating their utility as clinical biomarkers (Sánchez-Ovejero et al ., 2016).…”

Section: What Is the Next Step: Translational Applicationsmentioning

confidence: 99%

A new landscape of host–protozoa interactions involving the extracellular vesicles world

et al. 2018

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Extracellular vesicles (EVs) are released by a wide number of cells including blood cells, immune system cells, tumour cells, adult and embryonic stem cells. EVs are a heterogeneous group of vesicles (~30-1000 nm) including microvesicles and exosomes. The physiological release of EVs represents a normal state of the cell, raising a metabolic equilibrium between catabolic and anabolic processes. Moreover, when the cells are submitted to stress with different inducers or in pathological situations (malignancies, chronic diseases, infectious diseases.), they respond with an intense and dynamic release of EVs. The EVs released from stimulated cells vs those that are released constitutively may themselves differ, both physically and in their cargo. EVs contain protein, lipids, nucleic acids and biomolecules that can alter cell phenotypes or modulate neighbouring cells. In this review, we have summarized findings involving EVs in certain protozoan diseases. We have commented on strategies to study the communicative roles of EVs during host-pathogen interaction and hypothesized on the use of EVs for diagnostic, preventative and therapeutic purposes in infectious diseases. This kind of communication could modulate the innate immune system and reformulate concepts in parasitism. Moreover, the information provided within EVs could produce alternatives in translational medicine.

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Sensing parasites: Proteomic and advanced bio-detection alternatives

Cited by 24 publications

References 157 publications

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

A new landscape of host–protozoa interactions involving the extracellular vesicles world

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