Sensitive and rapid liquid chromatography/tandem mass spectrometric assay for the quantification of piperaquine in human plasma

Singhal, Puran; Gaur, Abhijeet; Gautam, Anirudh; Varshney, Brijesh; Paliwal, Jyoti; Batra, Vishal

doi:10.1016/j.jchromb.2007.09.021

Cited by 25 publications

(19 citation statements)

References 13 publications

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“…Literature survey reveals Automated Solid Phase Extraction and Liquid Chromatographic Method 3 , Capillary Zone Electrophoresis 4 , HPLC 5 and LC/MS/MS [6][7][8][9][10][11][12] methods for the estimation of Piperaquine phosphate alone or in combination with other drugs in pharmaceutical formulation and biological samples where as headspace gas chromatographic 13 methods for the estimation of Arterolane maleate alone in pharmaceutical formulation and biological samples. There are very few reported methods for the simultaneous determination of Arterolane maleate and Piperaquine phosphate by RP-HPLC in pharmaceutical dosage forms 14,15 .…”

Section: Fig 2: Structure Of Piperaquine Phosphatementioning

confidence: 99%

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2014

IJPSR

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ABSTRACT:A novel, precise, accurate, rapid and cost effective isocratic reverse-phase high performance liquid chromatographic (RP-HPLC) method was developed, optimized and validated for the estimation of Arterolane maleate and Piperaquine phosphate in pharmaceutical dosage forms (tablet). The drugs were estimated using hypersil C18 (250 mm x 4.6 mm i.d-5 µm particle size) column. A mobile phase composed of phosphate buffer, acetonitrile, methanol in proportion of 40:30:30 v/v, at a flow rate of 1.0 ml/min was used for the separation. Detection was carried out at 244 nm. The linearity range obtained was 20-70 µg/ml for ART and 100-350 µg/ml for PIP with retention times ( R t) of 3.353 min and 2.389 min for ART and PIP respectively. The correlation coefficient values were found to be 0.999. Precession studies showed % RSD values less than 2 % for both the drugs in all the selected concentrations. The percentage recoveries of ART and PIP were in the range of 99.47-100.72% and 99.30-100.18% respectively. The assay results of ART and PIP were 99.57% and 99.75 % respectively. The limit of detection (LOD) and limit of quantification (LOQ) were 0.172 µg/ml 0.524 µg/ml for ART and 0.542 µg/ml and 1.641 µg/ml for PIP respectively. The method was validated as per the International Conference on Harmonization (ICH) guidelines. The proposed validated method was successfully used for the quantitative analysis of commercially available dosage form.

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Section: Fig 2: Structure Of Piperaquine Phosphatementioning

confidence: 99%

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2014

IJPSR

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“…The linear range is 0.27-16.23 mmol/L (0.25-15.0 mg/mL) and the CV is 1.4-6.3% for free AMP-B, while 1.08-108.22 mmol/L (1.0-100.0 mg/mL) and 4.0-8.9% for liposomal AMP-B, respectively [15]. [7,8], plasma [5,6,8] Chromolith Performance RP-18e (100 Â 4.6 mm) [5,6,8], Chromolith (50 Â 4.6 mm) [7] Clobazam, N-desmethylclobazam [5] lamotrigine [7,8], phenytoin [8], carbamazepine-10,11-expoide [8], felbamate [8], dihydro-10-hydroxycarbamazepine [8], phentobarbital [8], phenobarbital [8], carbamazepine [8], primidone [8], zonisamide [8] [ [9,10,15,20] Plasma [9, 10, 13-15, 17, 20], serum [12], CSF [14], dried blood spots [16], urine [12,18], whole blood [19] Chromolith C18 (100 Â 4.6 mm) [9], Chromolith Performance RP-18e (100 Â 4.6 mm) [10,[13][14][15],…”

Section: Antibiotics Antiviral Antifungal and Antimalarial Drugsmentioning

confidence: 99%

“…[ [16][17][18][19] Tenofovir [9,10], emtricitabine [10] [ 9,10] Cephalexine, cephadroxil, cefaclor, cefotaxim [12] Amoxicillin, clavulanic acid [13] Amphotericin B [15] Voriconazole [14] Piperaquine [16][17][18][19][20] Antipsychotics and antidepressants…”

Section: Antibiotics Antiviral Antifungal and Antimalarial Drugsmentioning

confidence: 99%

“…Even though piperaquine is an old drug, little is known about its pharmacokinetics and the levels required to prevent or mitigate malaria resistance. Four HPLC-UV methods [16][17][18][19] with UV set at 345 or 347 nm and an LC-MS/MS [20] have been reported with a chromatography time of 2-3 min. The LOQs for the assays range from 0.002 mmol/L (1.0 ng/mL) to 0.05 mmol/L (26.8 ng/mL) and CVs range from 1.3 to 13.7% [16][17][18][19][20].…”

Section: Hplc-ms/msmentioning

confidence: 99%

“…Four HPLC-UV methods [16][17][18][19] with UV set at 345 or 347 nm and an LC-MS/MS [20] have been reported with a chromatography time of 2-3 min. The LOQs for the assays range from 0.002 mmol/L (1.0 ng/mL) to 0.05 mmol/L (26.8 ng/mL) and CVs range from 1.3 to 13.7% [16][17][18][19][20]. Methods for many different matrices were developed including whole blood [19], dried blood spots [16], plasma [17,20], and urine [18].…”

Section: Hplc-ms/msmentioning

confidence: 99%

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Applications of monolithic columns in liquid chromatography‐based clinical chemistry assays

Bunch

Wang

2011

J of Separation Science

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Monolithic columns have slowly been applied to HPLC methods for clinical chemistry testing in the last 10 years. The application areas include therapeutic drug monitoring, drugs of abuse, vitamins, porphyrins, and steroids. In comparison with conventional particulate columns, the monolithic columns may offer shorter chromatography time, more robustness, and better resolution for certain analytes. The potential drawback of large mobile phase consumption may be improved with smaller id columns, which are currently on the market. Methods covered in this review are those searchable in PubMed up to December 2010. This review highlights the emergence of monolithic column technology in HPLC methods used for clinical chemistry testing. The goals of this review are threefold: (i) To identify the areas of clinical chemistry that analytical monolithic columns have been used in HPLC methods. (ii) To demonstrate the application of analytical monolithic columns in HPLC methods using different detection systems. (iii) To discuss the advantages and limitations of the monolithic columns compared with particulate columns in the clinical chemistry applications.

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Simultaneous determination of piperaquine and its N‐oxidated metabolite in rat plasma using LC‐MS/MS

Liu

Zang

Yang

et al. 2017

Biomedical Chromatography

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A sensitive and efficient liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of piperaquine (PQ) and its N-oxidated metabolite (PQ-M) in plasma. A simple protein precipitation procedure was used for sample preparation. Adequate chromatographic retention was achieved on a C column under gradient elution with acetonitrile and 2 mm aqueous ammonium acetate containing 0.15% formic acid and 0.05% trifluoroacetic acid. A triple-quadrupole mass spectrometer equipped with an electrospray source was set up in the positive ion mode and multiple reaction monitoring mode. The method was linear in the range of 2.0-400.0 ng/mL for PQ and 1.0-50.0 ng/mL for PQ-M with suitable accuracy, precision and extraction recovery. The lower limits of detection (LLOD) were established at 0.4 and 0.2 ng/mL for PQ and PQ-M, respectively, using 40 μL of plasma sample. The matrix effect was negligible under the current conditions. No effect was found for co-administrated artemisinin drugs or hemolysis on the quantification of PQ and PQ-M. Stability testing showed that two analytes remained stable under all relevant analytical conditions. The validated method was successfully applied to a pharmacokinetic study performed in rats after a single oral administration of PQ (60 mg/kg).

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Sensitive and rapid liquid chromatography/tandem mass spectrometric assay for the quantification of piperaquine in human plasma

Cited by 25 publications

References 13 publications

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Applications of monolithic columns in liquid chromatography‐based clinical chemistry assays

Simultaneous determination of piperaquine and its N‐oxidated metabolite in rat plasma using LC‐MS/MS

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