Sensitive and selective liquid chromatography–tandem mass spectrometry method for the quantification of azithromycin in human plasma

Nirogi, Ramakrishna; Kandikere, Vishwottam; Shukla, Manoj K.; Mudigonda, Koteshwara; Maurya, Sudarshan; Boosi, Ravikumar; Anjaneyulu, Y.

doi:10.1016/j.aca.2005.08.007

Cited by 44 publications

(38 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chen et al (2006) extracted analyte with methyl tert-butyl ether-hexane (50:50 v/v) and monitored [M + 2H] 2+ by liquid chromatography/mass spectrometry (LC/MS). Barrett et al (2005) used an ultrafilter to pretreat sample and Nirogi et al (2005) used solid-phase extraction to prepare plasma, analysis being performed by liquid chromatography/tandem mass spectrometry (LC/MS/MS). All employed complicated and time-consuming sample preparation methods.…”

Section: Azithromycinmentioning

confidence: 99%

Sensitive liquid chromatography/mass spectrometry assay for the quantification of azithromycin in human plasma

Liu

Huang

et al. 2007

Biomedical Chromatography

View full text Add to dashboard Cite

A simple, rapid, sensitive and specific liquid chromatography/electrospray ionization mass spectrometry method was developed and validated to quantify azithromycin in human plasma, using erythromycin as the internal standard (IS). A simple sample preparation method of protein precipitation with methanol was employed. Methanol, acetonitrile and water (12:30:58, v/v/v) were used as the isocratic mobile phase, with 0.1% formic acid and 0.1% ammonium acetate in water. Selected ion monitoring was specific for azithromycin and erythromycin. The assay was linear over the concentration range 4.69-600 ng/mL. The correlation coefficients for the calibration curves ranged from 0.9994 to 0.9998. The intra- and inter-day precisions, calculated from quality control samples, were less than 8.24%. The method was employed in a pharmacokinetic study after oral administration of 500 mg azithromycin dispersible tablet to 20 healthy volunteers.

show abstract

Section: Azithromycinmentioning

confidence: 99%

Sensitive liquid chromatography/mass spectrometry assay for the quantification of azithromycin in human plasma

Liu

Huang

et al. 2007

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…A short run time in the HPLC method always facilitate analysis of several samples per day as already published in some of the studies with HPLC methods, (13,(28)(29)(30). The run time of 2.5 min in this study also facilitates analysis of several hundred samples of TNF per day.…”

Section: Introductionmentioning

confidence: 75%

Sensitive and Rapid HPLC Quantification of Tenofovir from Hyaluronic Acid-Based Nanomedicine

Agrahari

Youan

2012

AAPS PharmSciTech

View full text Add to dashboard Cite

Abstract. The purpose of this study was to develop and validate a rapid, sensitive, and specific reversedphase high-performance liquid chromatography method for the quantitative determination of native tenofovir (TNF) for various applications. Different analytical performance parameters such as linearity, precision, accuracy, limit of quantification (LOQ), limit of detection (LOD), and robustness were determined according to International Conference on Harmonization (ICH) guidelines. A Bridge™ C18 column (150×4.6 mm, 5 μm) was used as stationary phase. The retention time of TNF was 1.54±0.03 min (n=6). The assay was linear over the concentration range of 0.1-10 μg/mL. The proposed method was sensitive with LOD and LOQ values equal to 50 and 100 ng/mL, respectively. The method was accurate with percent mean recovery from 95.41% to 102.90% and precise as percent RSD (relative standard deviation) values for intra-day, and inter-day precision were less than 2%. This method was utilized for the estimation of molar absorptivity of TNF at 259 nm (ε 259 =12,518 L/mol/cm), calculated from linear regression analysis. The method was applied for determination of percentage of encapsulation efficiency (22.93±0.04%), drug loading (12.25±1.03%), in vitro drug release profile in the presence of enzyme (43% release in the first 3 h) and purification analysis of hyaluronic acid-based nanomedicine.

show abstract

“…9, 2010 most employed technique for this purpose. Hence, methods based on HPLC with electrochemical, 9,10 fluorimetric (with pre-column derivatization) 11,12 and mass spectrometric 13,14 detection were already reported. On the other hand, the development of HPLC methods with UV detection is not a common subject because of the extremely low absorption capacity of the azithromycin in this spectral range.…”

Section: Introductionmentioning

confidence: 99%

Novel spectrophotometric method for the determination of azithromycin in pharmaceutical formulations based on its charge transfer reaction with quinalizarin

Paula¹,

Almeida²,

Cassella³

2010

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Neste trabalho é proposto um novo método para a determinação espectrofotométrica simples e rápida de azitromicina em formulações farmacêuticas. O método está baseado na reação de transferência de carga entre a azitromicina e a quinalizarina em meio de metanol. O efeito de diversas variáveis foi avaliado com o intuito de alcançar máxima sensibilidade analítica. A reação de transferência de carga foi caracterizada, sendo determinadas a estequiometria da reação, a absortividade molar aparente e a constante de associação. Melhores resultados foram obtidos em meio de metanol e com uma concentração de quinalizarina de 50 mg L -1 . Nestas condições, o ânion radicalar (produto da reação de transferência de carga) foi formado imediatamente após a mistura dos reagentes, apresentando máxima absorção em 564 nm. O método apresentou um limite de detecção de 0,35 mg L -1 e um limite de quantificação de 1,2 mg L -1 , sendo aplicado com sucesso na determinação de azitromicina em três amostras de medicamentos comerciais contendo azitromicina. This paper proposes a new method for simple and fast spectrophotometric determination of azithromycin in pharmaceutical formulations. The method is based on the charge transfer reaction between the azithromycin and quinalizarin in methanol medium. In order to achieve maximum sensitivity the effect of some chemical variables such as the type of solvent, reagent concentration and reaction time were evaluated. The reaction was characterized in terms of stability of the product formed and its stoichiometry, and the apparent molar absorptivity and association constant were derived. Best conditions for the analytical determination of azithromycin were observed in methanol medium with a quinalizarin concentration of 50 mg L -1 . At these conditions, the radical anion (absorbing specie) was formed in the medium immediately after mixing of the reagents and showed maximum absorption at 564 nm. The method presented a limit of detection of 0.35 mg L -1 and a limit of quantification of 1.2 mg L -1 . It was successfully applied in the determination of azithromycin in three commercial pharmaceutical formulations of azithromycin and no matrix interferences were observed.

show abstract

Sensitive and selective liquid chromatography–tandem mass spectrometry method for the quantification of azithromycin in human plasma

Cited by 44 publications

References 19 publications

Sensitive liquid chromatography/mass spectrometry assay for the quantification of azithromycin in human plasma

Sensitive liquid chromatography/mass spectrometry assay for the quantification of azithromycin in human plasma

Sensitive and Rapid HPLC Quantification of Tenofovir from Hyaluronic Acid-Based Nanomedicine

Novel spectrophotometric method for the determination of azithromycin in pharmaceutical formulations based on its charge transfer reaction with quinalizarin

Contact Info

Product

Resources

About