Sensitive Biosensors Based on (Dendrimer Encapsulated Pt Nanoparticles)/Enzyme Multilayers

Zhu, Yihua; Zhu, Huiyu; Yang, Xin; Xu, Lihuan; Li, Chunzhong

doi:10.1002/elan.200603802

Cited by 53 publications

(24 citation statements)

References 27 publications

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“…The inset of Figure 1 shows a HR-TEM image of a single Pt nanoparticle and the single crystal structure was observed. Similar results have been reported in the literate [19,20,29].…”

Section: Characterization Of the Pt Nanoparticlessupporting

confidence: 92%

Properties of Dendrimer‐Encapsulated Pt Nanoparticles Doped Polypyrrole Composite Films and Their Electrocatalytic Activity for Glucose Oxidation

Tang

Zhu

et al. 2007

Electroanalysis

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A type of novel electroanalytical sensing nanobiocomposite material was prepared by electropolymerization of pyrrole containing poly(amidoamine) dendrimers-encapsulated platinum nanoparticles (Pt-PAMAM), and glucose oxidase (GOx). The Pt nanoparticles encapsulated in PAMAM are nearly monodisperse with an average diameter of 3 nm, and they provide electrical conductivity. Polypyrrole acts as a polymer backbone to give stable and homogeneous cast thin films, and it also defines the electrical conductivity. Both Polypyrrole and PAMAM can provide a favorable microenvironment to keep the bioactivity of enzymes such as glucose oxidase. The homogeneity of GOx/Pt-PAMAM-PPy nanobiocomposite films was characterized by atomic force microscopy (AFM). Amperometric biosensors fabricated with these materials were characterized electrochemically using cyclic voltammetry (CV) , electrochemical impedance spectra (EIS) and amperometric measurements in the presence of hydrogen peroxide or glucose. All those show the resultant biosensor sensitivity was strongly enhanced within the nanobiocomposite film. The optimized glucose biosensor displayed a sensitivity of 164 mA mM À1 cm À1 , a linear range of 0.2 to 600 mM, a detection limit of 10 nM, and a response time of < 3 s.

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“…The inset of Figure 1 shows a HR-TEM image of a single Pt nanoparticle and the single crystal structure was observed. Similar results have been reported in the literate [19,20,29].…”

Section: Characterization Of the Pt Nanoparticlessupporting

confidence: 92%

Properties of Dendrimer‐Encapsulated Pt Nanoparticles Doped Polypyrrole Composite Films and Their Electrocatalytic Activity for Glucose Oxidation

Tang

Zhu

et al. 2007

Electroanalysis

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“…However, the enzyme molecules that are mainly dispersed on the PNC surface or kept distance from the PNCs may suffer more severely from environmental perturbation, which gives rise to a more obvious loss of bioactivity during usage and storage of the relevant biosensors. [20,[22][23][24][25] Hence, the enzyme molecules entrapped in the present PBNCs are expected to show a higher sensitivity and longer storage ability, as discussed below.…”

Section: Fabrication and Characterization Of The Pbnc Filmsmentioning

confidence: 99%

“…In addition, the sensitivities of the proposed PBNC-based biosensors, especially for the PDA-GOxPtNP/Au electrode, are notably higher than those for the reported PNC-based biosensors (<31 mA cm À2 mM À1 ). [20][21][22][23][24][25] We also presynthesized PtNPs, [38] which were then mixed with GOx and DA to electrodeposit enzyme films on Au by a similar cyclic voltammetry (pre-synthesized PtNP-PDA-GOx/Au). Alternately www.afm-journal.de we synthesized PtNP-PDA composites by first reacting DA with H 2 PtCl 6 , and then adding GOx to interact with the composites, and the mixture was subjected to a similar cyclic voltammetry to grow enzyme films on Au ((PtNP-PDA)-GOx/Au).…”

Section: The Performance Of the Pbnc Film-based Enzyme Electrodesmentioning

confidence: 99%

One‐Pot Preparation of Polymer–Enzyme–Metallic Nanoparticle Composite Films for High‐Performance Biosensing of Glucose and Galactose

Xie

et al. 2009

Adv Funct Materials

139

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New polymer–enzyme–metallic nanoparticle composite films with a high‐load and a high‐activity of immobilized enzymes and obvious electrocatalysis/nano‐enhancement effects for biosensing of glucose and galactose are designed and prepared by a one‐pot chemical pre‐synthesis/electropolymerization (CPSE) protocol. Dopamine (DA) as a reductant and a monomer, glucose oxidase (GOx) or galactose oxidase (GaOx) as the enzyme, and HAuCl4 or H2PtCl6 as an oxidant to trigger DA polymerization and the source of metallic nanoparticles, are mixed to yield polymeric bionanocomposites (PBNCs), which are then anchored on the electrode by electropolymerization of the remaining DA monomer. The prepared PBNC material has good biocompatibility, a highly uniform dispersion of the nanoparticles with a narrow size distribution, and high load/activity of the immobilized enzymes, as verified by transmission/scanning electron microscopy and electrochemical quartz crystal microbalance. The thus‐prepared enzyme electrodes show a largely improved amperometric biosensing performance, e.g., a very high detection sensitivity (99 or 129 µA cm−2 mM−1 for glucose for Pt PBNCs on bare or platinized Au), a sub‐micromolar limit of detection for glucose, and an excellent durability, in comparison with those based on conventional procedures. Also, the PBNC‐based enzyme electrodes work well in the second‐generation biosensing mode. The proposed one‐pot CPSE protocol may be extended to the preparation of many other functionalized PBNCs for wide applications.

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“…By using dendrimers as both monodisperse templates and stabilizers, many metal nanocrystals had been prepared by the confined reduction. Dendrimer encapsulated Pt nanoparticles with uniform size have been synthesized in our laboratory which assumed as the novel electroanalytical sensing nanobiocomposite material [37][38][39][40][41][42][43][44][45]. Figure 7 gives the assembly process and the dendrimer encapsulated Pt nanoparticles.…”

Section: Confined Assembly and Application Of Nanocompositesmentioning

confidence: 99%

Structure controlling and process scale-up in the fabrication of nanomaterials

2010

Front. Chem. Eng. China

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Nanotechnology is already having a significant commercial impact, and will very certainly have a much greater impact in the future. The research on process engineering and scale-up will be very important for the commercial production and application of nanomaterials, because the properties and structure of nanomaterials are not only determined by the nucleation and growth process, but also strongly affected by the engineering properties, such as the mixing, the heat and mass transfer, and also the distribution of temperature, concentration, etc. This paper will present some research work in our laboratory on the fabrication of nanomaterials. Based on the chemical engineering principle and methods, many kinds of novel nanomaterials can be synthesized and their structure can be easily controlled through adjusting the parameters of the fluid mixing, and the distribution of temperature, residence time and concentration, etc. By using the micro-mixing, heat and mass transfer and reaction control methods, the host-guest nanocomposites have been assembled and assumed as the novel electroanalytical sensing nanobiocomposite materials. Based on the principles of chemical engineering, the manufacturing technologies for magnetic powders, calcium carbonate, and titanium dioxide have been developed for commercial-scale production, and the largest production scale has reached 15 kt/year.

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Sensitive Biosensors Based on (Dendrimer Encapsulated Pt Nanoparticles)/Enzyme Multilayers

Cited by 53 publications

References 27 publications

Properties of Dendrimer‐Encapsulated Pt Nanoparticles Doped Polypyrrole Composite Films and Their Electrocatalytic Activity for Glucose Oxidation

Properties of Dendrimer‐Encapsulated Pt Nanoparticles Doped Polypyrrole Composite Films and Their Electrocatalytic Activity for Glucose Oxidation

One‐Pot Preparation of Polymer–Enzyme–Metallic Nanoparticle Composite Films for High‐Performance Biosensing of Glucose and Galactose

Structure controlling and process scale-up in the fabrication of nanomaterials

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