Sensitive column-switching high-performance liquid chromatography method for determination of propiverine in human plasma

Ban, Eunmi; Maeng, Jeong-Eun; Woo, Jong Soo; Kim, Chong‐Kook

doi:10.1016/j.jchromb.2005.12.005

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…Propiverine hydrochloride (CAS 54556-98-8),abenzylic acid derivative,isananticholinergicagentwithits effectiveness in treating detrusorhyperreflexiaa nd overactivebladders ymptoms [1].The urospasmolytica ction of the drugisduetothe inhibition of calciuminflux into smoothm uscle cellsand competitiveantagonismwith acetylcholine atmuscarinicreceptors ites [2].…”

Section: Introductionmentioning

confidence: 99%

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Determination of propiverine hydrochloride in human plasma by high performance liquid chromatographytandem mass spectrometry: application to the pharmacokinetic study of a sustained release formulation

Huang

Tian

Zhang

et al. 2011

Arzneimittelforschung

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A rapid, sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the determination of propiverine hydrochloride (CAS 54556-98-8) in human plasma using cetirizine di-hydrochloride as internal standard (IS, CAS 8388-51-0). Following liquid-liquid extraction with ethyl acetate, the separation was performed on a reverse phase C18 column with a mobile phase consisted of metha-nol-ammonium acetate (pH 4.0; 10 mM) (70:30, v/v). The detection was performed by a triple-quadrupole mass spectrometer in the positive ion and multiple reaction monitoring (MRM) mode, m/z 368.3 ? 116.1 for propiverine and m/z 389.2 ? 201.0 for the IS. The calibration curve fitted well over the concentration range of 0.2?200 ng/mL (all the concentration data in this study are related to salt (propiverine hydrochloride)). The limit of detection (LOD) and lower limit of quantification (LLOQ) in human plasma were 0.05 and 0.2 ng/mL, respectively. The method was proved to be rapid, sensitive, specific, accurate and reproducible and has been successfully applied to a pharmacokinetic study of propiverine hydrochloride sustained release capsules (the 30 mg dose in this

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Section: Introductionmentioning

confidence: 99%

“…[4]established aH PLC method toassaypropiverine and its main metabolite( propiverine N-oxide) and Ban etal. [2]proposed ac olumn-switching HPLC method fort he quantification of propiverine. Nevertheless,the analysist ime of the HPLC methodsw ast oo long.…”

Section: Introductionmentioning

confidence: 99%

Determination of propiverine hydrochloride in human plasma by high performance liquid chromatographytandem mass spectrometry: application to the pharmacokinetic study of a sustained release formulation

Huang

Tian

Zhang

et al. 2011

Arzneimittelforschung

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“…[3][4][5][6][7] The detection of drugs in biological samples normally involves a sample treatment step before injection into the HPLC system. Typically, the extraction of drugs is performed using protein precipitation by organic solvents, [8][9][10] liq- uid-liquid extraction 11,12 and off-line SPE. [13][14][15][16] However, these conventional procedures are tedious, complicated and drugs may be partly lost in the sample preparation steps.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Caffeine and Theophylline in Human Plasma with a Weak Cation Monolithic SPE‐column

Zhu

Row²

2010

Chin. J. Chem.

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A simple reversed-phase high performance liquid chromatography (RP-HPLC) method was developed to determine the level of caffeine and theophylline in human plasma samples. The sample clean-up step involved the on-line solid-phase extraction (SPE) of the analytes from plasma samples into a weak cation monolithic column using a column switching system. Separation was performed on a C 18 column (5 µm, 150 mm×4.6 mm) with ultraviolet detection at 274 nm. The mobile phase consisted of methanol-water (32/68, V/V) under isocratic conditions at a flow rate of 0.6 mL•min -1 . The measured concentration of caffeine and theophylline showed a good linear relationship over the concentrations range, 0.1-80.0 µg•mL -1 . The absolute recoveries ranged from 77.10% to 85.39%, and the inter-day and intra-day relative standard deviations (RSD) were all less than 5%. This method avoids a tedious pretreatment and provides an economic, repeatable and effective method for assaying trace drugs in biological samples.

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“…Typical treatments include precipitation by acids or organic solvents [1], liquid-liquid extraction (LLE) [2][3][4][5][6] and off-line solid phase extraction (SPE) [7][8][9]. These conventional procedures are complicate, time-consuming, not friendly to environment, and drugs may be partly lost in sample preparation steps.…”

Section: Introductionmentioning

confidence: 99%

On-line clean-up and screening of oxacillin and cloxacillin in human urine and plasma with a weak ion exchange monolithic column

Yang

Feng

Liu

et al. 2007

Journal of Chromatography B

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Sensitive column-switching high-performance liquid chromatography method for determination of propiverine in human plasma

Cited by 6 publications

References 14 publications

Determination of propiverine hydrochloride in human plasma by high performance liquid chromatographytandem mass spectrometry: application to the pharmacokinetic study of a sustained release formulation

Determination of propiverine hydrochloride in human plasma by high performance liquid chromatographytandem mass spectrometry: application to the pharmacokinetic study of a sustained release formulation

Simultaneous Determination of Caffeine and Theophylline in Human Plasma with a Weak Cation Monolithic SPE‐column

On-line clean-up and screening of oxacillin and cloxacillin in human urine and plasma with a weak ion exchange monolithic column

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