Sensitive competitive direct enzyme-linked immunosorbent assay and gold nanoparticle immunochromatographic strip for detecting aflatoxin M1 in milk

Wang, Jing-Jhih; Liu, Biing-Hui; Hsu, Yu-Tien; Yu, Feng‐Yih

doi:10.1016/j.foodcont.2010.12.003

Cited by 93 publications

(66 citation statements)

References 18 publications

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“…Liu et al (2016) observed in their analytical results that four of the 17 samples were contaminated with very low concentrations of AFM1 and that all concentrations of AFM1 were below the regulatory limits established by the FDA of Taiwan and the USA (0.5 ng / mL) and the level in a sample slightly exceeded the regulatory limit established in the European Union (0.05 ng / mL). This study was consistent with a study where pAb-based cdELISA was used to analyze milk (Wang et al, 2011). Chadseesuwan et al (2016) also observed in their research for aflatoxin M1 in fortified raw milk that the samples were within the acceptable range of detection.…”

Section: Resultssupporting

confidence: 91%

Determination of aflatoxin M1 in bovine milk from the Alagoas/Brazil State dairy belt by high performance liquid chromatography (HPLC)

Alves¹,

Lima²,

Costa³

et al. 2018

Afr. J. Microbiol. Res.

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Milk is considered a nutritionally noble food and is therefore suitable for feeding children and adults. However, contamination of milk by mycotoxins may pose a health risk to the consumer. Aflatoxins, mycotoxins produced by fungi of the genus Aspergillus, can be found in several food products, including milk and its derivatives, which reinforces the importance of this type of study on the occurrence of the aflatoxin M 1 (AFM 1 ) in raw milk. We analyzed 45 samples of raw bovine milk from expansion tanks from 23 farms in different municipalities of the dairy belt of the state of Alagoas/Brazil. Samples were collected directly from the cooling tanks and transported under refrigeration for analysis. The method used for the extraction of AFM 1 was that proposed by the Adolfo Lutz Institute. On the other hand, the detection of AFM 1 occurred by high performance liquid chromatography (HPLC) by identifying retention times. The results of the analyses indicated that none of the collected samples presented contamination by aflatoxin M 1 , thus indicating that the milk commercialized in Alagoas shows a good quality against this toxic agent.

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Section: Resultssupporting

confidence: 91%

Determination of aflatoxin M1 in bovine milk from the Alagoas/Brazil State dairy belt by high performance liquid chromatography (HPLC)

Alves¹,

Lima²,

Costa³

et al. 2018

Afr. J. Microbiol. Res.

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“…We conclude, therefore, that the observed emission spectra of QDs with aflatoxins are not attributable simply to the presence of aflatoxins, but to the specific interaction between aflatoxins and the CdS QDs. All types of aflatoxin have carbonyl groups capable of reacting with the amine functional groups presented by cysteamine, as described previously [54]. Figure 7 demonstrates an example of this reaction with aflatoxin B1 and cysteamine.…”

Section: Effect Of Time On Photostability Of Cysteamine-capped Cds Qdsmentioning

confidence: 71%

Determination of total aflatoxin using cysteamine-capped CdS quantum dots as a fluorescence probe

et al. 2016

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Aflatoxins form a class of potent carcinogens that contaminate a wide range of food products and can be fatal to humans and livestock. We have designed cysteamine-capped CdS quantum dots (QDs) to serve as aflatoxin photodetectors for use in agricultural industries. Water-soluble CdS QDs are synthesized through growth in a poly(vinyl alcohol) matrix using a chemical precipitation method. The prepared QDs are then characterized with X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), and transmission electron microscopy (TEM) analyses. The obtained results revealed that these CdS QDs have a 1-to 2-nm crystalline size, hexagonal wurtzite structure, and spherical morphology with a diameter less than 10 nm. Photoluminescence spectroscopy (PL) is performed to study the CdS QDs interactions with a standard solution of aflatoxins (B1, B2, G1, and G2 in a ratio of 5:1:5:1) in order to determine their effectiveness as aflatoxin detectors. A green emission peak is observed at 508 nm, with an intensity enhancement positively correlated with total aflatoxin concentration. The lower limit of detection for total aflatoxin concentration is found to be 0.05 ppb, well below international contamination allowances for food products. PL variations with aflatoxin concentration are best described by a Langmuir-type equation in the concentration range of this study (2.4-48 ppb).

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“…Some authors have successfully described LFDs for aflatoxins that work well with corn [18], grains and feedstuffs [25,26] and milk [27], but require strip treatment before use. A new LFD for aflatoxins that did not require special pre-treatment was developed by Masinde et al [19] and has been commercialised as the Aflatoxin Quicktest™ by QuickTest Technologies.…”

Section: Poisoning -From Specific Toxic Agents To Novel Rapid and Simmentioning

confidence: 99%

The Aflatoxin QuicktestTM—A Practical Tool for Ensuring Safety in Agricultural Produce

Sánchez‐Bayo¹,

Almeida²,

Williams³

et al. 2017

Poisoning - From Specific Toxic Agents to Novel Rapid and Simplified Techniques for Analysis

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Contamination of corn, peanuts, milk and dairy products with aflatoxins is a worldwide problem, particularly in subtropical regions where the climatic conditions are ideal for the growth of Aspergillus flavus, the fungi that produces these toxins. Developing countries have major difficulties in marketing these products abroad due to the stringent international regulations concerning this carcinogenic toxin. Adding to the problem is the analytical cost involved in monitoring the produce, which require sophisticated instrumentation and qualified personnel, neither of which are available for field testing. The development of a rapid Aflatoxin Quicktest™ provides an effective, reliable and cheaper option for screening levels of aflatoxin above the regulatory thresholds in such produce. The test consists of a lateral flow device (LFD) coated with antibodies specific to aflatoxin B1, although it detects other aflatoxins (i.e. G and M) with high cross-reactivity. Its high sensitivity allows analysis of these toxins in the range 2-40 μg/kg of sample in 15 minutes, plus the time for extraction, which varies among different products. Quantification of the test results is done using a Quick Reader, by comparing the readings of individual tests against a standard curve of the analytes in the same manner as it is done with any other analytical equipment. A validation study was carried out using peanuts from Australia and peanuts and corn from Timor-Leste to assess the performance of the Aflatoxin Quicktest™. Results obtained with the LFD showed a good correlation with the standard analytical measurements by HPLC-fluorescence (r 2 above 0.90 for all cases), indicating the Aflatoxin Quicktest™ is capable of measuring levels of aflatoxins accurately and reliably. Given their ease of use, low cost and fast processing time, the Aflatoxin Quicktest™ can be used for screening agricultural produce in countries that cannot afford the costly alternative of using specialised personnel and equipment.

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Sensitive competitive direct enzyme-linked immunosorbent assay and gold nanoparticle immunochromatographic strip for detecting aflatoxin M1 in milk

Cited by 93 publications

References 18 publications

Determination of aflatoxin M1 in bovine milk from the Alagoas/Brazil State dairy belt by high performance liquid chromatography (HPLC)

Determination of aflatoxin M1 in bovine milk from the Alagoas/Brazil State dairy belt by high performance liquid chromatography (HPLC)

Determination of total aflatoxin using cysteamine-capped CdS quantum dots as a fluorescence probe

The Aflatoxin QuicktestTM—A Practical Tool for Ensuring Safety in Agricultural Produce

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