Sensitive electrochemical immunoassay based on polythionine-Au nanocomposites as enhanced sensing signal for selective detection of biomarker with high isoelectric point

An ultrasensitive electrochemical immunosensor for the detection of human immunoglobulin G based on Ag@BSA microspheres

“…4,5 The performance of the electrochemical immunosensor was greatly inuenced by the electron transfer efficiency and biocompatibility of materials modied on the electrode surface. 6,7 From this point of view, noble metal nanoparticles were frequently chosen for the sensing interface and tracing tags. [3][4][5][6] However, it was a challenge to decrease the background signal produced in the absence of antigens which oen restricted the detection limit of the immunosensor.…”

A simple fluorescence assay for trypsin through a protamine-induced carbon quantum dot-quenching aggregation platform

“…At present, considerable efforts have been devoted to developing efficient and reliable methods for Try detection based on various strategies, including colorimetry, 8 electrochemistry, 9 and uorescence methods. 10 Fluorescent approaches have been widely used due to their characteristics of simplicity, rapidity, sensitivity, and realizing real-time detection.…”

Simple and effective label-free electrochemical immunoassay for carbohydrate antigen 19-9 based on polythionine-Au composites as enhanced sensing signals for detecting different clinical samples

“…37,38 Regulation of polythionine can improve the ability and application performance in electrochemical biosensors. [39][40][41][42] Anionic surfactant-doped polythionine with sensitive response was recently prepared and used for the fabrication of label-free electrochemical immunosensor. 39 The previous work reported that polythionine and gold nanocomposites can be effectively synthesized and applied to detect biomarkers with high isoelectric point.…”

“…39 The previous work reported that polythionine and gold nanocomposites can be effectively synthesized and applied to detect biomarkers with high isoelectric point. 40 Although reported works illustrated the fact that specific electrochemical biosensors for some targets based on polythionine or its composites can be obtained, the construction of facile label-free electrochemical sensing interface for large-scale analysis of biomarkers for variable clinical samples is still not available.…”

“…40 The modulated surface structure and surface charge promoted the current transfer between the electrode and the electrolyte in a total sensing loop and improved the response current. 46 Subsequently, when the electrode was modified with BSA to eliminate nonspecific binding, the peak current decreased because of the formation of an electron-blocking layer.…”