Sensitive ELISA for IgG and IgM anti-albumin autoantibodies not influenced by HBsAG-associated polyalbumin receptors

Nardiello, S; Pizzella, T; Russo, Massimo; Galanti, B

doi:10.1016/0022-1759(85)90484-3

Cited by 6 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(ii) pHSA-RIA is nonspecifically inhibited by normal human serum in large range of dilutions as noted here and by the others (5), probably by the presence of anti-pHSA antibody in these sera (16). Our data suggest that although the expression of the pre-S-coded epitopes on viral particles parallels the expression of pHSA binding sites and its reaction with specific (anti-pre-S) antibody is blocked by soluble pHSA, the sites of fixation of pHSA and anti-pre-S are not the same.…”

Section: Different Reference Sera Of S(1) S(2) S(3) S(4) S(5)mentioning

confidence: 79%

Section: Different Reference Sera Of S(1) S(2) S(3) S(4) S(5)mentioning

confidence: 82%

“…Digestion of the reference preparation of recombinant particles in a concentration of 16 HBsAg and anti-HBs, HBeAg and anti-HBe, anti-HBc were determined by commercially available RIA kits from Abbott Laboratories. HBV-specific DNA polymerase activity was assayed according to Alberti et al (13).…”

Section: X 100mentioning

confidence: 99%

“…RIA involving anti-pre-S in solid phase seems to have some advantages over the other techniques for detection of pHSA binding sites and anti-pre-S antibody: (i) the hemagglutination method cannot differentiate antialbumin antibodies and pHSAR activity (16) and nonspecific blocking of the hemagglutination reaction by sera without any HBV markers (especially from patients with lupus erythematosus); this has been observed recently in our laboratory (17). (ii) pHSA-RIA is nonspecifically inhibited by normal human serum in large range of dilutions as noted here and by the others (5), probably by the presence of anti-pHSA antibody in these sera (16). Our data suggest that although the expression of the pre-S-coded epitopes on viral particles parallels the expression of pHSA binding sites and its reaction with specific (anti-pre-S) antibody is blocked by soluble pHSA, the sites of fixation of pHSA and anti-pre-S are not the same.…”

Section: Heermann Et Almentioning

confidence: 99%

See 3 more Smart Citations

Hepatitis B virus pre-S gene-encoded antigenic specificity and anti-pre-S antibody: Relationship between anti-pre-S response and recovery

et al. 1986

View full text Add to dashboard Cite

A solid-phase radioimmunoassay involving specific antibody was developed for determination of the pre-S gene-encoded epitopes of hepatitis B virus and anti-pre-S antibody in sera of hepatitis B patients. The reaction for pre-S determinants associated with HBsAg was quantitatively inhibited by soluble, polymerized human serum albumin, and the lower limit of the assay was about 1.6 ng of HBsAg per ml. Continuous expression of pre-S-coded antigenic sites on HBsAg particles in chronic hepatitis B patients seropositive for HBeAg or anti-HBe shows that these determinants may be considered as a marker of chronicity during hepatitis B virus infection. The anti-pre-S antibody was determined by inhibition of the reaction for pre-S determinants. This antibody, different from anti-HBs, was detected during HBsAg antigenemia in patients recovering from acute type B hepatitis, before anti-HBs response. Kinetics of synthesis of anti-pre-S antibody in the course of acute type B hepatitis, followed by elimination of HBsAg and recovery, suggest the possible role of this antibody in the immunological clearance of infective hepatitis B virus particles.

show abstract

Section: Different Reference Sera Of S(1) S(2) S(3) S(4) S(5)mentioning

confidence: 79%

Section: Different Reference Sera Of S(1) S(2) S(3) S(4) S(5)mentioning

confidence: 82%

Section: X 100mentioning

confidence: 99%