Sensitive high-performance liquid chromatographic method with fluorometric detection for the simultaneous determination of gabapentin and vigabatrin in serum and urine

Simultaneous determination of vigabatrin and amino acid neurotransmitters in brain microdialysates by capillary electrophoresis with laser-induced fluorescence detection

“…Since vigabatrin does not have a chromophore or fluorophore in its structure, HPLC methods with a UV/Vis detector exhibit poor detection limits [7]. Consequently, O-pthaldialdehyde (OPA) [8,9], Dns chloride [10] and 4-chloro-7-nitrobenzofurazan (NBD-Cl) [11] have been used as pre-column derivatizing agents for HPLC with fluorescence detection assays of vigabatrin. Recently, a CE-LIFD method for determining vigabatrin in human plasma after pre-column derivatization with tetramethylrhodamine succinimidyl ester has been published [12].…”

A Simple High-Throughput Method for Determination of Antiepileptic Analogues of γ-Aminobutyric Acid in Pharmaceutical Dosage Forms Using Microplate Fluorescence Reader

“…1) The analytical method development for PGB, GBP and VGB determination is challenging since these molecules have no significant UV or visible absorption. Several derivatization methods have been proposed [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] and used for their determination in bulk or pharmaceutical dosage forms, plasma, serum, and urine. These methods employed derivatization reagents such as fluorescamine, [2][3][4][17][18][19] 7-chloro-4-nitrobenzofurazan, [5][6][7] 1-fluoro-2,4-dinitrobenzene, 8) o-phtaldialdehyde, 9,10) 2,4,6-trinitrobenzene sulfonic acid, 11,12) and 9-fluorenylmethylchloroformate.…”

“…2,[5][6][7]17,19) Moreover, HPLC 3,[8][9][10][11][12][13][14]18) and capillary electrophoresis (CE) methods 4) using precolumn derivatization with either UV or fluorescent detector were also used.…”

Kinetic and Thermodynamic Spectrophotometric Technique to Estimate Gabapentin in Pharmaceutical Formulations using Ninhydrin

“…Its action is attributed to the irreversible inhibition of the enzyme GABA-transaminase, thus preventing the physiological degradation of GABA in the brain (Ouellet et al 2001). Analytical methods reported for its determination consist of high-performance liquid chromatography (HPLC) (Jiang & Li 1999;Tang et al 1999;Chollet et al 2000;Ifa et al 2001;Ratnaraj & Patsalos 1998;Wad & Kramer 1998), spectrofluorimetry (Belal et al 2002;Hassan et al 2001), gas chromatography-mass spectrometry (GC-MS) (Kushnir et al 1999;Van Lentea & Gatautis 1998), capillary electrophoresis (Rada et al 1998) and spectrophotometry applying Hantzsch reaction (al-Zehouri et al 2001). So far, no traces of any attempts have been found for determination of gabapentin by colorimetric method and the literature is still starving for such analytical procedures.…”