Sensitive Immunodetection Through Impedance Measurements onto Gold Functionalized Electrodes

Ameur, Sami; Martelet, C.; Jaffrézic‐Renault, Nicole; Chovelon, Jean‐Marc

doi:10.1385/abab:89:2-3:161

Cited by 30 publications

(16 citation statements)

References 14 publications

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“…Ameur et al used a SAM-functionalized gold electrode in a flow cell with various routes to antibody immobilization in an early faradaic study [202]. They reported detection limits of 5 -10 pg/mL depending on functionalization route.…”

Section: Faradaic Studiesmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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Impedance biosensors are a class of electrical biosensors that show promise for point-of-care and other applications due to low cost, ease of miniaturization, and label-free operation. Unlabeled DNA and protein targets can be detected by monitoring changes in surface impedance when a target molecule binds to an immobilized probe. The affinity capture step leads to challenges shared by all label-free affinity biosensors; these challenges are discussed along with others unique to impedance readout. Various possible mechanisms for impedance change upon target binding are discussed. We critically summarize accomplishments of past label-free impedance biosensors and identify areas for future research.

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Section: Faradaic Studiesmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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“…Similar capacitance measurements were applied for quantitative immunoanalysis [51]. For example, anti-rabbit IgG antibodies coupled to a self-assembled cystamine monolayer on a Au electrode were used for the detection of the respective antigen in a linear range between 0.5 ± 8.5 ng mL À1 based on the analysis of capacitance changes by non-Faradaic impedance spectroscopy [73].…”

Section: Immunosensors Based On Interfacial Impedance Measurements Atmentioning

confidence: 99%

Probing Biomolecular Interactions at Conductive and Semiconductive Surfaces by Impedance Spectroscopy: Routes to Impedimetric Immunosensors, DNA‐Sensors, and Enzyme Biosensors

2003

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Impedance spectroscopy is a rapidly developing electrochemical technique for the characterization of biomaterialfunctionalized electrodes and biocatalytic transformations at electrode surfaces, and specifically for the transduction of biosensing events at electrodes or field-effect transistor devices. The immobilization of biomaterials, e.g., enzymes, antigens/antibodies or DNA on electrodes or semiconductor surfaces alters the capacitance and interfacial electron transfer resistance of the conductive or semiconductive electrodes. Impedance spectroscopy allows analysis of interfacial changes originating from biorecognition events at electrode surfaces. Kinetics and mechanisms of electron transfer processes corresponding to biocatalytic reactions occurring at modified electrodes can be also derived from Faradaic impedance spectroscopy. Different immunosensors that use impedance measurements for the transduction of antigen-antibody complex formation on electronic transducers were developed. Similarly, DNA biosensors using impedance measurements as readout signals were developed. Amplified detection of the analyte DNA using Faradaic impedance spectroscopy was accomplished by the coupling of functionalized liposomes or by the association of biocatalytic conjugates to the sensing interface providing biocatalyzed precipitation of an insoluble product on the electrodes. The amplified detections of viral DNA and single-base mismatches in DNA were accomplished by similar methods. The changes of interfacial features of gate surfaces of field-effect transistors (FET) upon the formation of antigen-antibody complexes or assembly of protein arrays were probed by impedance measurements and specifically by transconductance measurements. Impedance spectroscopy was also applied to characterize enzymebased biosensors. The reconstitution of apo-enzymes on cofactor-functionalized electrodes and the formation of cofactor-enzyme affinity complexes on electrodes were probed by Faradaic impedance spectroscopy. Also biocatalyzed reactions occurring on electrode surfaces were analyzed by impedance spectroscopy. The theoretical background of the different methods and their practical applications in analytical procedures were outlined in this article.

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“…The electroconductive matrix presents a high surface concentration of grafted biomolecules and the change of conformation brought by biological recognition induces a change of the polymer conjugation, which results in amplification of the electrical phenomenon. A particular enhancement of the sensitivity of detection was observed in presence of redox probe [34]. EIS, under appropriate experimental conditions, represents a label-free technique for immunodetection.…”

Section: B Electrochemical Impedance Spectroscopy (Eis) Labelfree Dementioning

confidence: 98%

A Novel Point of Care Diagnostic Device: Impedimetric Detection of a Biomarker in Whole Blood

McMurray¹,

Ali

Kyselovik

et al. 2007

2007 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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Sensitive Immunodetection Through Impedance Measurements onto Gold Functionalized Electrodes

Cited by 30 publications

References 14 publications

Label‐Free Impedance Biosensors: Opportunities and Challenges

Label‐Free Impedance Biosensors: Opportunities and Challenges

Probing Biomolecular Interactions at Conductive and Semiconductive Surfaces by Impedance Spectroscopy: Routes to Impedimetric Immunosensors, DNA‐Sensors, and Enzyme Biosensors

A Novel Point of Care Diagnostic Device: Impedimetric Detection of a Biomarker in Whole Blood

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