Sensitive liquid chromatography/tandem mass spectrometry method for the simultaneous determination of nine local anesthetic drugs

Tonooka, Keiko; Naruki, Nobuhiko; Honma, Kou; Agei, Kohei; Okutsu, Mayumi; Hosono, Tetsuji; Kunisue, Yoko; Terada, Masaru; Tomobe, Koji; Shinozuka, Tatsuo

doi:10.1016/j.forsciint.2016.02.044

Cited by 28 publications

(13 citation statements)

References 12 publications

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“…They allow simultaneous quantification of LA and their metabolites in the same mixture and can be used for the analysis of biological objects and foodstuffs. However, these methods are not always available and require expensive equipment and reagents [5][6][7]. There are also simple and cheap spectrophotometric methods [8][9][10][11][12] that are significantly less selective and sensitive than chromatographic ones.…”

Section: Introductionmentioning

confidence: 99%

A New Approach for the Determination of Benzocaine and Procaine in Pharmaceuticals by Single-Sweep Polarography

Plotycya

Strontsitska

Pysarevska

et al. 2018

International Journal of Electrochemistry

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A new polarographic method for the determination of benzocaine and procaine based on the polarographic reduction of their chemically obtained oxidation products with potassium peroxymonosulfate is developed. Experimental conditions affecting quantitative yield of benzocaine and procaine oxidation products such as рH, oxidation time, reagents’ concentration, and temperature are explored. It is shown that the reduction current changes in a linear fashion (R=0.999) with increasing concentration of anesthetics over a concentration range of 1·10−6 - 5·10−5 mol L−1. The calculated limits of detection (LOD) for benzocaine and procaine are found to be 5.6·10−6 and 6·10−6 mol L−1, respectively. In the present study, quantitative polarographic determination of benzocaine in Farisil tablets and “Septolete Plus” lozenges and procaine in solution for injections is performed. The results of the analysis are in good agreement with the product specifications described in the quality certificates. The possibility of quantitative determination of benzocaine and procaine in pharmaceuticals is confirmed.

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Section: Introductionmentioning

confidence: 99%

A New Approach for the Determination of Benzocaine and Procaine in Pharmaceuticals by Single-Sweep Polarography

Plotycya

Strontsitska

Pysarevska

et al. 2018

International Journal of Electrochemistry

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“…This study used mass spectrometry, an established, robust technique for post-operative quantification of the local anesthetic in plasma [5]. As demonstrated by the product ion spectra for bupivacaine and the deuterated internal control D9-bupivacaine (Figure 1), although lo- cal anesthetics can be very close in size and chemistry, they can be distinguished from one another by chromatography [6]. The data in this study are in general agreement with the gas chromatography-mass spectrometry data described by Odor et al [7].…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetic Profile of Plasma Levobupivacaine Following Fascia Iliaca Compartment Block for Proximal Femoral Fracture in Older Patient

Parr¹,

Robinson²,

Cameron³

et al. 2020

PBR

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Background: Fascia Iliaca Compartment Block (FICB) is commonly used in older patients to provide effective analgesia following hip fracture. Objectives: However, only limited Pharmacokinetic (PK) data about levobupivacaine are available to help clinical practice and establish safe volumes and amounts of local anesthetics.Methods: Ten patients aged between 53 and 87 years, who underwent hemiarthroplasty following femoral neck fracture were recruited into this study. A fixed volume (40 mL) of 0.25% levobupivacaine was injected before the induction of anesthesia using ultrasound guidance. Venous blood samples were obtained at 0, 10, 20, 30, 45, 60, 75, 90, and 120 min time points and analyzed using mass spectrometry. Results: The median (interquartile range) maximum observed plasma concentration (Cmax) of levobupivacaine was 0.48 (0.45-0.61) µg/mL, with the time to reach Cmax (tmax) of 38 minutes (30–105) after administration, a half-life of 2.8 h (1.65–5.8), and clearance rate of 0.72 L/min (0.36–1.26). The fixed volume (40 mL) of 0.25% levobupivacaine FICB did not exceed the recognized toxic threshold in adults (2.6 µg/mL). Conclusion: The data described here indicate a similar levobupivacaine PK profile for older patients undergoing FICB for hip arthroplasty compared with the levobupivacaine PK profile for the general population.

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“…This paper was an analysis of mepivacaine only, since the objective was to compare if there was difference of absorption in the anterior and posterior region of the maxilla. The sensitive and selective method offers the opportunity for the simultaneous screening and quantification, for clinical and forensic purposes, of almost all local anesthetics ( 10 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the plasmatic level of mepivacaine in different anatomical regions

Ferreira

Catunda²,

Vasconcelos³

et al. 2018

Med Oral

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BackgroundTo evaluate the serum level of the local anesthetic mepivacaine 3% without vasoconstrictor in patients who underwent procedures performed in the anterior and posterior maxilla, through a method of possible extraction to quantify it in human plasma by high performance liquid chromatography (HPLC).Material and MethodsThis was a hybrid study consisting of 18 patients (7 females and 11 males) classified as ASA I, adults and with normal body mass index, submitted to procedures in the anterior region (group I) and posterior region of the maxilla (group II). For 40 minutes, five 6 ml blood samples were collected every 10 minutes after infiltrative injection in each region of the maxilla. Serum levels of the drug were obtained through HPLC. Blood pressure (BP) and heart rate (HR) were measured throughout the procedure.ResultsWhen compared to the general average of the concentrations of each group, significant values (p<0.05) with greater absorption were observed for the anterior region of the maxilla (group I). There was no significant difference when comparing blood pressure (BP) and heart rate (HR) values.ConclusionsThe concentrations found are safe for infiltrative anesthesia in the analyzed patients, there was a higher plasma level of the local anesthetic in the anterior region of the maxilla and there was no change in HR and BP in relation to the anesthetized area. Key words:Local anesthetic. toxicity. mepivacaine. chromatography. chromatography, high pressure liquid.

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Sensitive liquid chromatography/tandem mass spectrometry method for the simultaneous determination of nine local anesthetic drugs

Cited by 28 publications

References 12 publications

A New Approach for the Determination of Benzocaine and Procaine in Pharmaceuticals by Single-Sweep Polarography

A New Approach for the Determination of Benzocaine and Procaine in Pharmaceuticals by Single-Sweep Polarography

Pharmacokinetic Profile of Plasma Levobupivacaine Following Fascia Iliaca Compartment Block for Proximal Femoral Fracture in Older Patient

Evaluation of the plasmatic level of mepivacaine in different anatomical regions

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