Sensitive Simultaneous Detection of Seven Sexually Transmitted Agents in Semen by Multiplex-PCR and of HPV by Single PCR

Gimenes, Fernanda Raphael Escobar; Medina, Fabiana Soares; Abreu, André Luelsdorf Pimenta de; Irie, Mary Mayumi Taguti; Esquiçati, Isis Baroni; Malagutti, Natália; Vasconcellos, Vinícius Rodrigo Bulla; Discacciati, Michelle Garcia; Bonini, Marcelo G.; Maria‐Engler, Silvya Stuchi; Consolaro, Márcia Edilaine Lopes

doi:10.1371/journal.pone.0098862

Cited by 65 publications

(49 citation statements)

References 48 publications

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Section: Pcr Typementioning

confidence: 99%

“…Multiplex PCR is a clear choice to meet this need, as it is a rapid and accurate method that can be used to simultaneously screen for multiple sexually transmitted agents. Two scientific research teams thoroughly investigated ways to improve the detection rate of several diseases concurrently. The results show that multiplex PCR has a favorable consistency with single PCR or FilmArray; thus, multiplex PCR is a complementary point‐of‐care choice in the future but still needs further research and practice.…”

Section: Introductionmentioning

confidence: 99%

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PCR detection for syphilis diagnosis: Status and prospects

Zhou

Zhang

et al. 2019

Clinical Laboratory Analysis

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Syphilis, a re‐emerging public health problem worldwide caused by Treponema pallidum subsp pallidum (T. pallidum), usually induces systemic and chronic inflammation in hosts who do not receive timely therapy after exposing to high‐risk factors such as leprous sexual contact. Before the treatment, rapid and accurate detection of syphilis is essential. However, the existing detection methods, which focus on the treponemal or non‐treponemal antibody test, both have inherent limitations. For instance, both of them cannot distinguish the stage and severity of syphilis. Non‐treponemal test such as RPR, which is generally deemed to be used for assessing treatment response, is influenced by biological false positives. Therefore, it is imperative to seek out a new and effective diagnostic test. With recent advancements in molecular biology and whole‐genome sequencing, the molecular diagnosis has increased in popularity, especially the use of polymerase chain reaction (PCR). Here, we firstly present a mini‐review on the research of PCR detection methods used for syphilis diagnosis over the past decade, and we then compare these methodologies to assess their potential and the challenges faced. This information can provide a fresh perspective to help researchers address the current challenges.

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Section: Pcr Typementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PCR detection for syphilis diagnosis: Status and prospects

Zhou

Zhang

et al. 2019

Clinical Laboratory Analysis

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“…An expanded window for diagnosis in CHIKV is also needed since IgM seropositivity may persist for months after the initial acute infection. Many other viruses have been detected in semen, such as Ebola, HIV, HBV, HPV, HSV-1, HSV-2, Marburg, but there has been no published data so far on the presence of CHIKV in semen [13], [14], [15], [16], [17].…”

Section: Introductionmentioning

confidence: 99%

Prolonged shedding of Chikungunya virus in semen and urine: A new perspective for diagnosis and implications for transmission

Bandeira

Campos

Rocha³

et al. 2016

IDCases

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AbsractWe report the presence of Chikungunya (CHIKV) RNA in the blood, urine and semen during the acute phase of the disease in an adult with a dual infection with Dengue virus type 3. The patient, a 25 yr-old man from Salvador, Brazil, reported a 6-day duration of high fever, arthralgia, myalgia, headache and photophobia.Blood and semen specimens were positive for CHIKV in the first collected samples; semen and urine specimens were positive for CHIKV after 30 days of symptoms onset. DENV-3 RNA was positive in blood specimen when first collected 6 days after the initiation of symptoms.We describe for the first time the presence of CHIKV RNA in urine and semen for an extended period of time and we address the possible implications of these findings for diagnosis and transmission dynamics.

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“…10 The final volume (25 uL) of PCR reaction consisted of 2.5 uL of 10X PCR-buffer, 100 ng of DNA, 3 mM MgCl 2 , 200 uM of each dNTP, and 1 U of Taq polymerase (Bioline, London, UK).…”

mentioning

confidence: 99%

Sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in Jalisco, Mexico

Casillas-Vega

Morfín‐Otero

García

et al. 2016

SALUD PÚBLICA DE MÉXICO

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Objective. To determine the frequency of nine sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in Jalisco, Mexico. Materials and methods. Samples from 662 patients attending obstetrics and gynecology clinics were analyzed. Treponema pallidum, HIV, and HCV were detected by serology. HPV was detected by Polimerase Chain Reaction (PCR), and its genotype was determined by Restriction Fragment Length Polymorphism (RFLP). Trichomonas vaginalis, HSV-1, HSV-2, Mycoplasma genitalium, Neisseria gonorrhoeae and T. pallidum were detected by multiplex PCR. Results. By serology, HIV frequency was 6.8%, T. pallidum was 2.26%, and HCV was 0.15%. By PCR, HPV frequency was 13.9%, (more frequent genotype was 16, 33.7%), followed by T. vaginalis (14.2%), HSV-1 (8.5%), M. genitalium (2,41%), N. gonorrhoeae (2.11%), HSV-2 (1.8%), and T. pallidum (1.05%). Patients

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Sensitive Simultaneous Detection of Seven Sexually Transmitted Agents in Semen by Multiplex-PCR and of HPV by Single PCR

Cited by 65 publications

References 48 publications

PCR detection for syphilis diagnosis: Status and prospects

PCR detection for syphilis diagnosis: Status and prospects

Prolonged shedding of Chikungunya virus in semen and urine: A new perspective for diagnosis and implications for transmission

Sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in Jalisco, Mexico

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