Sensitive spectrofluorimetric assay based on micelle enhanced protocol for the determination of hepatitis C antiviral agent (simeprevir): Application to dosage form and human plasma

Mohammed, Bassam Shaaban; El‐Malla, Samah F.; Derayea, Sayed M.

doi:10.1016/j.microc.2019.104372

Cited by 13 publications

(10 citation statements)

References 17 publications

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“…The resolution of the overlaid spectra was allowed by using Δλ value of 90 nm, which is not equal to the difference between the emission and excitation wavelengths of the two drugs. Therefore, the sensitivity of the proposed method is slightly lower than the native estimation reported in the literature 9,25 . However, it is higher than another fluorimetric method reported for SIM 25 .…”

Section: Limit Of Quantitation (Loq) and Limit Of Detection (Lod)mentioning

confidence: 61%

“…Accuracy and precision. The suggested method's accuracy was confirmed by statistically analyzing the obtained data compared to those obtained using reference methods 9,25 . The Student t-test and the Variance ratio F-test were used to statistically examine the data 43 .…”

Section: Limit Of Quantitation (Loq) and Limit Of Detection (Lod)mentioning

confidence: 80%

“…For the analysis of SIM, the literature review reveals several analytical methods for its assay, whether alone or in combination. These methods involve spectrophotometric 22,23 , chemometric-assisted spectrophotometric 24 , spectrofluorimetric [25][26][27] , and LC methods [28][29][30][31][32][33] .…”

Section: Simultaneous Spectrofluorimetic Determination Of Remdesivir ...mentioning

confidence: 99%

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Simultaneous spectrofluorimetic determination of remdesivir and simeprevir in human plasma

Sharkasy

Tolba

Belal

et al. 2022

Sci Rep

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As new infectious mutations of SARS-CoV-2 emerged throughout the world, innovative therapies to counter the virus-altered drug sensitivities were urgently needed. Several antiviral options have been in clinical trials or in compassionate use for the treatment of SARS-CoV-2 infections in an attempt to minimize both clinical severity and viral shedding. Recent research indicated that simeprevir acts synergistically with remdesivir, allowing for a multiple-fold decrease in its effective dose when used at physiologically acceptable concentrations. The goal of this work is to develop a sensitive synchronous spectrofluorimetric approach to simultaneously quantify the two drugs in biological fluids. Using this method, remdesivir and simeprevir could be measured spectrofluorimetrically at 283 and 341 nm, respectively, without interference from each other using Δλ of 90 nm. The effect of various experimental parameters on the fluorescence intensity of the two drugs was extensively explored and optimized. For each of remdesivir and simeprevir, the method exhibited a linearity range of 0.10–1.10 μg/mL, with lower detection limits of 0.01 and 0.02 μg/mL and quantification limits of 0.03 and 0.05 μg/mL, respectively. The high sensitivity of the developed method permitted the simultaneous determination of both drugs in spiked plasma samples with % recoveries ranging from 95.0 to 103.25 with acceptable standard deviation values of 1.92 and 3.04 for remdesivir and simeprevir, respectively. The validation of the approach was approved by the International Council of Harmonization (ICH) guidelines.

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Section: Limit Of Quantitation (Loq) and Limit Of Detection (Lod)mentioning

confidence: 61%

Section: Limit Of Quantitation (Loq) and Limit Of Detection (Lod)mentioning

confidence: 80%

Section: Simultaneous Spectrofluorimetic Determination Of Remdesivir ...mentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous spectrofluorimetic determination of remdesivir and simeprevir in human plasma

Sharkasy

Tolba

Belal

et al. 2022

Sci Rep

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“…[ 23 ] This strategy has been utilized to enhance the sensitivity of fluorimetry of many pharmaceutical compounds. [ 24–26 ] MTX in aqueous solution has weak original fluorescence properties at 675 nm if excited at 610 nm (Figure 1). Different surfactants and macromolecules were examined for their ability to strengthen the weak MTX fluorescence emission.…”

Section: Resultsmentioning

confidence: 99%

Investigating the interaction of mitoxantrone with anionic surfactants by spectrofluorimetry and its application for the feasible analysis of pharmaceutical preparation and biological fluids

2020

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The behaviour of mitoxantrone (MTX), an anthracenedione antineoplastic agent, in different types of organized medium was explored using molecular spectrofluorometry. The original fluorescence and quantum yield of MTX were augmented by about five‐fold in the aqueous buffered solution (Britton–Robinson, pH 3.0) by the addition of sodium dodecyl sulfate. Enhancement in the fluorescence intensity did not come from the boost in the ultraviolet (UV) light absorbance of the drug in the presence of micelles but due to shielding of the lowest excited singlet state of the drug from a radiationless process inside the cavity of the micelle. Accordingly, a versatile, sensitive, and feasible spectrofluorimetric method was constructed and evaluated for MTX determination. Fluorescence measurements were performed at 675 nm (λex 610 nm). A linear relationship was shown between fluorescence intensity and drug concentration within the range 0.01–2.0 μg ml−1 of MTX with a correlation coefficient of 0.9999 and a detection limit of 2 ng ml−1. The developed method was effectively used for analysis of MTX in biological samples and dosage forms. In addition, the method was expanded to study the stability of MTX exposed to different drastic degradations and the kinetic parameters of the degradation were calculated.

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“…The literature review of SMV [9] , [10] , [11] , [12] , [13] and SFV [14] , [15] , [16] , [17] , [18] revealed few published methods for determination of the drugs individually. There were only two published methods for the simultaneous determination goal [19] , [20] .…”

Section: Introductionmentioning

confidence: 99%

Application of different spectrophotometric methods for quantitative analysis of direct acting antiviral drugs simeprevir and sofosbuvir

Ramzy

Abdelazim

2022

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Sensitive spectrofluorimetric assay based on micelle enhanced protocol for the determination of hepatitis C antiviral agent (simeprevir): Application to dosage form and human plasma

Cited by 13 publications

References 17 publications

Simultaneous spectrofluorimetic determination of remdesivir and simeprevir in human plasma

Simultaneous spectrofluorimetic determination of remdesivir and simeprevir in human plasma

Investigating the interaction of mitoxantrone with anionic surfactants by spectrofluorimetry and its application for the feasible analysis of pharmaceutical preparation and biological fluids

Application of different spectrophotometric methods for quantitative analysis of direct acting antiviral drugs simeprevir and sofosbuvir

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