Sensitive spectrophotometric method for determination of catecholamines in pure and pharmaceutical formulations

Abstract

Accepted Received 5/12/2006 11/9/2006 ‫ال‬ ‫ملخص‬ ‫أمينات‬ ‫أدوية‬ ‫من‬ ‫امية‬ ‫مايكروغر‬ ‫كميات‬ ‫لتقدير‬ ‫وحساسة‬ ‫سهمة‬ ‫طيفية‬ ‫يقة‬ ‫طر‬ ‫تطوير‬ ‫تم‬ ‫ينالين‬ ‫ادر‬ ‫و‬ ‫ودوبامين‬ ‫دوبا‬ ‫بالمثيل‬ ‫المتمثمة‬ ‫الكاتيكول‬ . ‫األدوية‬ ‫تمك‬ ‫أكسدة‬ ‫عمى‬ ‫يقة‬ ‫الطر‬ ‫تعتمد‬ ‫الكاشف‬ ‫مع‬ ‫الناتج‬ ‫الثنائي‬ ‫الحديد‬ ‫ومفاعمة‬ ‫حامضي‬ ‫وسط‬ ‫في‬ ‫الثالثي‬ ‫الحديد‬ ‫بوساطة‬ 10،1 -‫في‬ ‫األدوية‬ ‫تمك‬ ‫تقدير‬ ‫في‬ ‫بنجاح‬ ‫يقة‬ ‫الطر‬ ‫تطبيق‬ ‫أمكن‬ ‫و‬ ‫اغ‬ ‫السو‬ ‫اد‬ ‫مو‬ ‫وجود‬ ‫من‬ ‫تداخالت‬ ‫الصيدالنية‬ ‫اتها‬ ‫مستحضر‬ . AbstractA sensitive and simple spectrophotometric method is developed for the determination of catecholamine drugs namely; methyldopa, dopamine and adrenaline in pure and pharmaceutical preparations. The method is based on the oxidation of the cited drugs with iron(III) in acidic medium and the liberated iron(II) reacts with 1,10-phenanthroline. The ferroin complex is colorimetrically measured at 510 nm against reagent blank. Beer's law was valid over the concentration ranges of 0.1-2.8, 0.1-2.0, and 0.2-6.0μg/ml and the molar absorptivities are 1.851×10 5 , 1.544×10 5 and 4.331×10 4 l.mol -1 cm -1 for methyldopa, dopamine and adrenaline respectively. The common excipients and additives did not interfere in their determinations. The proposed method was successfully applied to the assay of the above drugs in various dosage forms.

Spectrophotometric Determination of Catecholamine Containing Drugs Using Calcon Dye

“…Various spectrophotometric methods using different reagents have been reported for the determination of catecholamine drugs. These methods include ion association using eosin Y and application of cloud point extraction technique [26], charge transfer complexes using bromanil [27], oxidation-reduction using ferric ion [28][29][30], diazotisation using pnitroaniline [31], 4-aminoantipyrine in the basic medium [32], complexation reaction using 4-aminoantipyrine and copper ion, ionpair complex using triiodide ion [33] and using ferrous ion as a complexing agent in alkaline medium [34], oxidative coupling using p-toluidine in the presence of sodium periodide [35], 2, 6-diaminopyridine in the presence of potassium periodate [36] and 3methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate in the presence of potassium ferricyanide [37].…”