Sensitive Two-Dimensional Determination of Small Amounts of D-Amino Acids in Mammals and the Study on Their Functions

Hamase, Kenji

doi:10.1248/cpb.55.503

Cited by 31 publications

(26 citation statements)

References 42 publications

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“…Ultra-high performance liquid chromatography methods for the determination and analysis of disease biomarkers including some chiral methods for AAs were clearly reviewed [41]. Several review papers focused on the presence of D-AAs in mammals, their function, their diagnostic value as biomarkers, and also HPLC methods for their determination [42][43][44][45][46][47]. Examples of D-AAs related to some diseases are summarized in Table 1.The enantioseparation of D,L-AAs continues to be a subject of immense study due to its significant importance in various areas.…”

Section: D-amino Acids As Biomarkers Of Diseases/disordersmentioning

confidence: 99%

Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement

2016

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Abstract:Occurrence of D-amino acids in living organisms is a useful indicator of various changes, diseases, or disorders. Determination of amino acid enantiomers, namely the enantiomeric ratio of amino acids or excess of certain D-amino acids, represents a useful tool in the studies of aging processes or biomarkers in disease/disorder diagnosis in humans. The amount of D-amino acids is usually very low. Therefore, suitable sample pretreatment, often derivatization, and highly selective and sensitive separation methods are essential for D-amino acid analysis in this field. Chromatographic techniques offer appropriate choices for solving these tasks. This review covers the advances in methodology and development of improved instrumental chromatographic methods focused on D,L-amino acid separation and determination. New findings in the area of possible D-amino acid biomarkers are also included.

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Section: D-amino Acids As Biomarkers Of Diseases/disordersmentioning

confidence: 99%

Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement

2016

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“…Newer analytical techniques have allowed the determination of trace levels of D-Thr in other physiologic tissues amidst excessive L-isomers (Hamase et al 2009;Waldhier et al 2010). The four isomers of Thr were resolved using a two-dimensional HPLC system and measured in various tissue of mammals (Hamase 2007;Zhao et al 2004). D-Allo-Thr was found in the brain and the urine at much higher levels than D-Thr.…”

Section: D-serinementioning

confidence: 99%

Nutritional and medicinal aspects of d-amino acids

2011

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This paper reviews and interprets a method for determining the nutritional value of D-amino acids, D-peptides, and amino acid derivatives using a growth assay in mice fed a synthetic all-amino acid diet. A large number of experiments were carried out in which a molar equivalent of the test compound replaced a nutritionally essential amino acid such as L-lysine (L-Lys), L-methionine (L-Met), L-phenylalanine (L-Phe), and L-tryptophan (L-Trp) as well as the semi-essential amino acids L-cysteine (L-Cys) and L-tyrosine (L-Tyr). The results show wide-ranging variations in the biological utilization of test substances. The method is generally applicable to the determination of the biological utilization and safety of any amino acid derivative as a potential nutritional source of the corresponding L-amino acid. Because the organism is forced to use the D-amino acid or amino acid derivative as the sole source of the essential or semi-essential amino acid being replaced, and because a free amino acid diet allows better control of composition, the use of all-amino-acid diets for such determinations may be preferable to protein-based diets. Also covered are brief summaries of the widely scattered literature on dietary and pharmacological aspects of 27 individual D-amino acids, D-peptides, and isomeric amino acid derivatives and suggested research needs in each of these areas. The described results provide a valuable record and resource for further progress on the multifaceted aspects of D-amino acids in food and biological samples.

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“…A column switching HPLC approach has been valuable for determining trace D,L-amino acid levels in the CNS and was recently reviewed in part [50]. Column switching HPLC with fluorescence detection using a variety of individualized protocols (i.e.…”

Section: Gc/ms Approachesmentioning

confidence: 99%

“…The chiral component of interest can then be determined through online column coupling and detection. When micro-RP-HPLC is used for the first separation step, optimal sensitivity can be obtained for the fractionated band [50]. Major limitation relates to the cost of operating the system which requires two fluorescent detectors and multiple LC columns as well as analysis times which generally exceed 50 min per sample.…”

Section: Gc/ms Approachesmentioning

confidence: 99%

Separation and sensitive detection of D‐amino acids in biological matrices

Kirschner

Green

2009

J of Separation Science

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The increase in our understanding of D-amino acid function and distribution in mammals is in many ways a result of the initial development of sensitive enantioselective separation strategies that allow for quantification in real biological samples. This article reviews progress on the development of chiral selective separation and detection of D-amino acids including enzyme-based microbiosensors, GC/MS, HPLC/fluorescence, HPLC/MS-MS, cEKC/fluorescence, and MEKC/fluorescence. Only methods capable of analyzing D-isomers in biological matrices are given here and significant effort is made to highlight approaches that offer speed, resolution, high sensitivity, and versatility.

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Sensitive Two-Dimensional Determination of Small Amounts of D-Amino Acids in Mammals and the Study on Their Functions

Cited by 31 publications

References 42 publications

Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement

Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement

Nutritional and medicinal aspects of d-amino acids

Separation and sensitive detection of D‐amino acids in biological matrices

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