Sensitivity and Selectivity Analysis of Fluorescent Probes for Hydrogen Sulfide Detection

Abstract

Hydrogen sulfide (H 2 S) is a gasotransmitter known to regulate physiological and pathological processes. Abnormal H 2 S levels have been associated with a range of conditions, including Parkinson's and Alzheimer's diseases, cardiovascular and renal diseases, bacterial and viral infections, as well as cancer. Therefore, fast and sensitive H 2 S detection is of significant clinical importance. Fluorescent H 2 S probes hold great potential among the currently developed detection methods because of their high sensitivity, selectiv-ity, and biocompatibility. However, many proposed probes do not provide a gold standard for proper use and selection. Consequently, issues arise when applying the probes in different conditions. Therefore, we systematically evaluated four commercially available probes (WSP-1, WSP-5, CAY, and P3), considering their detection range, sensitivity, selectivity, and performance in different environments. Furthermore, their capacity for endogenous H 2 S imaging in live cells was demonstrated.

A H2S‐Generated Supramolecular Photosensitizer for Enhanced Photodynamic Antibacterial Infection and Relieving Inflammation

“…For further investigation, we detected the responsive release of H 2 S from TPP‐CN/CP5 in live bacterial cells using the H 2 S fluorescent probe Washington State Probe‐1 (WSP‐1). [ 18 ] After pretreating bacteria with different solutions and WSP‐1, the channel images of the H 2 S probe were monitored by fluorescence microscopy (Figure 3e ). As expected, almost no fluorescence was observed in the control group, and the bacteria exhibited weak fluorescence in the TPP‐CN/CP5 groups due to the release of a small amount of H 2 S triggered by endogenous Cys.…”

Impact of Reactive Sulfur Species on Entamoeba histolytica: Modulating Viability, Motility, and Biofilm Degradation Capacity

“…At 2.65 mM Na 2 S, we observed that 75% of the trophozoites were killed after one hour of exposure (Figure 1b). In order to monitor the H 2 S delivery into the parasite, we used fluorescent probe WSP-5 [48]. At a concentration of 2.65 mM Na 2 S, we observed a 1.4-fold increase in intracellular WSP-5 fluorescence compared to the untreated control trophozoites after 10 min of exposure.…”

Synthetic nanoprobes for biological hydrogen sulfide detection and imaging

“…As reported in our recent work, certain H 2 S fluorescent probes reported negligible responses to biothiols, while competing reactions between biothiols and H 2 S may still occur in complex biological samples. [206] Given the importance of H 2 S, clear discrimination between H 2 S, H 2 S mixtures with biothiols, and other signaling molecules should be accomplished.…”