Sensitivity and specificity of Congo red staining according to Romhányi. Comparison with Puchtler's or Bennhold's methods

Bély, M.; Makovitzky, Josef

doi:10.1016/j.acthis.2006.03.017

Cited by 58 publications

(33 citation statements)

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“…CR has been observed to bind to native proteins with various secondary structures [130,134], as well as partially unfolded proteins [130], protein oligomers [144], and lipids [145]. Notably, the sensitivity of CR staining can depend on the method used and may make for instance collagen fibers and cytoskeletal proteins produce a false-positive birefringence [130,146]. Finally, in a few cases, fibrils with a typical appearance of amyloid fibrils lack the ability to induce apple-green birefringence upon CR staining [147,148].…”

Section: Do Congo Red and Thioflavin T Have Similar Binding Modes?mentioning

confidence: 99%

Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils—current status

2009

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Because understanding amyloid fibrillation in molecular detail is essential for development of strategies to control amyloid formation and overcome neurodegenerative disorders, increased understanding of present molecular probes as well as development of new probes are of utmost importance. To date, the binding modes of these molecular probes to amyloid fibrils are by no means adequately described or understood, and the large number of studies on Thioflavin T (ThT) and Congo Red (CR) binding have resulted in models that are incomplete and conflicting. Different types of binding sites are likely to be present in amyloid fibrils with differences in binding modes. ThT may bind in channels running parallel to the long axis of the fibril. In the channels, ThT may bind in either a monomeric or dimeric form of which the molecular conformation is likely to be planar. CR may bind in grooves formed along the β-sheets as a planar molecule in either a monomeric or supramolecular form.

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Section: Do Congo Red and Thioflavin T Have Similar Binding Modes?mentioning

confidence: 99%

Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils—current status

2009

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“…Congo red has a high affinity for polysaccharides and has been used for the diagnosis of amyloidosis (Bély and Makovitzky 2006), the differentiation of pathogenic strains of Escherichia coli (Berkhoff and Vinal 1986), and the isolation of Azospirillum (Hernández et al 2000) as vital dye and roots for describing fungal tissues (Guzmán et al 1999). It is necessary to assess the amount of glucose produced by the endoglucanases from CMC.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Endoglucanase Activity based on Carboxymethyl Cellulose in Four Fungi Isolated from an Aerated Lagoon in a Pulp and Paper Mill

Ordaz-Díaz¹,

Rojas‐Contreras

Flores-Vichi³

et al. 2016

BioResources

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The aim of this study was to identify cellulolytic fungal strains capable of degrading cellulose from an aerated lagoon in a pulp and paper mill. Four fungal strains that were found to be highly active were isolated on carboxymethyl cellulose (CMC) and suggested to be CMCase/endoglucanase. The identified strains were Aspergillus niger, Penicillium sp., Aspergillus fumigatus, and Mucor sp. All the strains were studied in terms of cultural morphological characteristics and microscopic examinations. The endoglucanase with the highest isolate production was Penicillium sp., which also showed the highest qualitative endoglucanase activity (1.3 cm), in addition to the main activity of endoglucanase with 297 mmol/mg.min after 116 h. The results indicated that CMC is able to induce endoglucanase enzyme production and that the fungal isolates showed significant cellulose degradation properties.

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“…Esta capacidad de unirse a polisacáridos ha sido utilizada como herramienta diagnóstica en clínica y en otras aplicaciones (Pire 1985;Berkhoff & Vinal 1986;Guzman et al 1999;Hernández et al 2000;Bély et al 2006). Este medio de cultivo CMC-rojo Congo al ser expuesto a un hongo celulolítico rápidamente pierde viscosidad y absorbancia, debido a que las endoglucanasas rompen la estructura amorfa de la CMC (Ohmiya et al 2003;Aro et al 2005).…”

Section: Discussionunclassified

“…La técnica cualitativa más usada es la reportada por Teather & Wood (1982), que se basa en la asociación de rojo Congo y carboximetilcelulosa que genera una intensa coloración que se desvanece ante la actividad despolimerizante de la endoglucanasa. El rojo Congo tiene una alta afinidad por los polisacáridos y ha sido utilizado para el diagnóstico de la amiloidosis (Bély et al 2006), diferenciación de cepas patógenas de Escherichia coli (Berkhoff & Vinal 1986), para el aislamiento de Azospirillum (Hernández et al 2000), como colorante vital para raíces (Pire 1985) y para la descripción de tejidos fúngicos (Guzmán et al 1999).…”

Section: Introductionunclassified

Nuevo método para la cuantificación de la actividad endoglucanasa basado en el complejo celulosa-rojo congo

Moreno

Uribe-Vélez

2011

Orinoquia

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Ortiz -Nuevo método para la cuantificación de la actividad endoglucanasa RESUMENEl objetivo de este trabajo fue establecer un método de cuantificación para la actividad endoglucanasa basado en la despolimerización del complejo polisacárido-rojo Congo, con este fin se describió el comportamiento matemático de la viscosidad y la absorbancia del medio de cultivo carboximetilcelulosarojo Congo. Para medir el impacto de un hongo celulolítico como Trichoderma viride sobre el medio, se realizó el seguimiento de la viscosidad y la absorbancia del medio de cultivo inoculado por 8 días. El cálculo de la concentración de enzima se realizó correlacionando los resultados de pruebas de azúcares reductores por DNS con la pérdida de absorbancia del medio de cultivo CMC. Para evaluar la aplicabilidad de la técnica se probaron cuatro cepas de deuteromicetos celulolíticos y se encontró que el uso de la variable pérdida de absorbancia del medio CMC, fue efectiva para diferenciar la actividad endoglucanasa de las cepas. El método basado en rojo Congo-CMC es más rápido y sensible que el DNS. Se recomienda aplicar este nuevo método en los screening para la bioprospección de hongos celulolíticos. Palabras clave: Endoglucanasa, carboximetilcelulosa, rojo Congo, hongos celuloliticos. ABSTRACTThe aim of this work was to establish a method for quantifying endoglucanase activity based on the depolymerisation of the Congo red-polysaccharide complex. The mathematical viscosity and absorbance patternsforCongo red-carboxymethylcellulose(CMC) culture media were thus described. The inoculated media'sviscosity and absorbance were monitored for 8 daysto measure the impact of a cellulolytic fungus such as Trichodermaviride on the media. Enzymeconcentration was calculated by correlating reducing sugars'absorbance test results using3,5-dinitrosalicylic acid (DNS) with loss of CMC culture mediaabsorbance. Four cellulolytic deuteromycetesstrains were testedto assess the technique'sapplicability;it was found that usingCMC media absorbancelosswas effective for differentiatingstrains'endoglucanase activity. The Congored- CMC method was faster and more sensitive than the DNS method. This new method should thusbe appliedin screening assays when bioprospecting cellulolytic fungi in soil.

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Sensitivity and specificity of Congo red staining according to Romhányi. Comparison with Puchtler's or Bennhold's methods

Cited by 58 publications

References 6 publications

Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils—current status

Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils—current status

Quantification of Endoglucanase Activity based on Carboxymethyl Cellulose in Four Fungi Isolated from an Aerated Lagoon in a Pulp and Paper Mill

Nuevo método para la cuantificación de la actividad endoglucanasa basado en el complejo celulosa-rojo congo

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