Sensitivity enhancement in the fluorometric determination of aliphatic amines using naphthalene-2,3-dicarboxaldehyde derivatization followed by vortex-assisted liquid–liquid microextraction

Wang, Chin-Yi; Tung, Shu-Yin; Lo, Yuk Keung; Huang, Hsin-Yi; Ko, Chun-Han; Wu, Chueh‐Yu

doi:10.1016/j.talanta.2016.02.043

Cited by 14 publications

(6 citation statements)

References 38 publications

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“…The regression analysis for the calibration curves showed a good linear correlation over the range from 0.01 to 2.5 mg/L, with a coefficient of determination ( r 2 ) being better than 0.9998. The enrichment factor (EF) and the extraction recovery (R%) were calculated based on the ratio of slopes of calibration curve before and after VALLME . EF and R% were 4.16 and 78%, respectively.…”

Section: Resultsmentioning

confidence: 99%

Vortex‐assisted liquid–liquid microextraction of strontium from water samples using 4′,4″(5″)‐di‐(tert‐butylcyclohexano)‐18‐crown‐6 and tetraphenylborate

Wang

Chang

Shen

et al. 2017

J of Separation Science

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A vortex-assisted liquid-liquid microextraction method was developed for the chromatographic determination of strontium in aqueous samples. In the method, strontium was complexed with 4',4″(5″)-di-(tert-butylcyclohexano)-18-crown-6 in the presence of tetraphenylborate as the counter anion, which increased the hydrophobicity of the ion-association complex, resulting in its improved extraction into 1-octanol. Strontium from the organic phase was stripped with nitric acid back to aqueous solution and determined by ion chromatography. The optimum microextraction conditions were as follows: 2.0 mL aqueous samples with 3 mM tetraphenylborate; 150 μL of 1-octanol as the extractant phase with 10 mM DtBuCH18C6; vortex extraction time for 10 s; centrifugation at 6000 rpm for 4 min; stripping by 0.1 M nitric acid. Under the optimum conditions, the detection limit for strontium was 0.005 mg/L. The calibration curves showed good linearity over the range between 0.01 and 2.5 mg/L. Intra- and interday precisions of the present method were satisfactory with relative standard deviations of 1.7 and 2.1%, respectively.

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Section: Resultsmentioning

confidence: 99%

Vortex‐assisted liquid–liquid microextraction of strontium from water samples using 4′,4″(5″)‐di‐(tert‐butylcyclohexano)‐18‐crown‐6 and tetraphenylborate

Wang

Chang

Shen

et al. 2017

J of Separation Science

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“…Ultra-trace detection of primary amines has been achieved by derivatization with fluorescent reagents. An attractive flourogen of choice is naphthalene-2,3-dicarboxaldehyde (NDA), due to commercial availability, inexpensive cost, and simple reaction schema [43,44]. At equimolar concentrations, varying the reactant (primary amine, potassium cyanide (KCN), NDA) ratios directly affects the fluorescence response.…”

Section: Fluorescent Tagging Of Primary Aminesmentioning

confidence: 99%

Development of a Microfluidic Platform for Trace Lipid Analysis

Davic

Cascio

2021

Metabolites

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The inherent trace quantity of primary fatty acid amides found in biological systems presents challenges for analytical analysis and quantitation, requiring a highly sensitive detection system. The use of microfluidics provides a green sample preparation and analysis technique through small-volume fluidic flow through micron-sized channels embedded in a polydimethylsiloxane (PDMS) device. Microfluidics provides the potential of having a micro total analysis system where chromatographic separation, fluorescent tagging reactions, and detection are accomplished with no added sample handling. This study describes the development and the optimization of a microfluidic-laser induced fluorescence (LIF) analysis and detection system that can be used for the detection of ultra-trace levels of fluorescently tagged primary fatty acid amines. A PDMS microfluidic device was designed and fabricated to incorporate droplet-based flow. Droplet microfluidics have enabled on-chip fluorescent tagging reactions to be performed quickly and efficiently, with no additional sample handling. An optimized LIF optical detection system provided fluorescently tagged primary fatty acid amine detection at sub-fmol levels (436 amol). The use of this LIF detection provides unparalleled sensitivity, with detection limits several orders of magnitude lower than currently employed LC-MS techniques, and might be easily adapted for use as a complementary quantification platform for parallel MS-based omics studies.

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“…The obtained results also demonstrated that even in cases where the derivatization reaction is performed on the fiber, Although this analysis time is comparable or slightly higher with respect to other derivatization SPME protocols [2,14,[25][26][27], it has the advantage of avoiding the addition of the derivatization agent to each sample which, can potentially constitute an additional source of error in the analysis routine. On the other hand, other extraction techniques such as vortex assisted liquidliquid microextraction [28], micro extraction in packed syringe (MEPS) [29], single drop microextraction (SDME) [30], air-assisted liquid-liquid microextraction (AALLME) [9], in syringe derivatization [6] used for determination of derivatized amines often lack automation options, employ organic solvents and include tedious procedures.…”

Section: Optimization Of Reaction Conditions In Aqueous Phasementioning

confidence: 99%

A facile and fully automated on-fiber derivatization protocol for direct analysis of short-chain aliphatic amines using a matrix compatible solid-phase microextraction coating

Gionfriddo

Passarini

Pawliszyn

2016

Journal of Chromatography A

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Solid-phase microextraction (SPME) analysis of short-chain aliphatic amines (C3-C6) in aqueous solutions was investigated using pentafluorobenzaldehyde (PFBAY) as on-fiber derivatization reagent. A standard gas generating vial agent was used for on-fiber loading of the derivatization agent so as to avoid the need for its regeneration at each derivatization cycle. Several parameters such as loading time, reaction temperature, and reaction/extraction time were optimized for headspace and direct sampling in aqueous solutions. Three different coating chemistries were tested and their performances compared in order to achieve the best compromise between sensitivity and analysis throughput. The newly developed PDMS/DVB/PDMS coating showed superior performance in terms of extraction efficiency while the capability to prevent on-fiber degradation of the derivatizing products. The optimized method was used for quantitation of short-chain aliphatic amines in aqueous samples and provided detection limits in the low ppb range for all the amines tested with accuracy values between 79 and 120%. The method was applied towards the analysis of environmental water samples and the accuracy of the results was evaluated by different calibration approaches.

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Sensitivity enhancement in the fluorometric determination of aliphatic amines using naphthalene-2,3-dicarboxaldehyde derivatization followed by vortex-assisted liquid–liquid microextraction

Cited by 14 publications

References 38 publications

Vortex‐assisted liquid–liquid microextraction of strontium from water samples using 4′,4″(5″)‐di‐(tert‐butylcyclohexano)‐18‐crown‐6 and tetraphenylborate

Vortex‐assisted liquid–liquid microextraction of strontium from water samples using 4′,4″(5″)‐di‐(tert‐butylcyclohexano)‐18‐crown‐6 and tetraphenylborate

Development of a Microfluidic Platform for Trace Lipid Analysis

A facile and fully automated on-fiber derivatization protocol for direct analysis of short-chain aliphatic amines using a matrix compatible solid-phase microextraction coating

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