Sensitivity of fluorophore-quencher labeled microbubbles to externally applied static pressure

Yuan, Baohong

doi:10.1118/1.3158734

Cited by 6 publications

(8 citation statements)

References 54 publications

(232 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A very innovative application of fluorescently labeled MB was presented by Yuan in 2009 [105]. Here, a fluorophore quencher-labelled MB system was used to measure external pressure.…”

Section: Multimodal Us Imagingmentioning

confidence: 99%

Recent advances in molecular, multimodal and theranostic ultrasound imaging

Kießling

Fokong

Bzyl

et al. 2014

Advanced Drug Delivery Reviews

182

151

View full text Add to dashboard Cite

Ultrasound (US) imaging is an exquisite tool for the non-invasive and real-time diagnosis of many different diseases. In this context, US contrast agents can improve lesion delineation, characterization and therapy response evaluation. US contrast agents are usually micrometer-sized gas bubbles, stabilized with soft or hard shells. By conjugating antibodies to the microbubble (MB) surface, and by incorporating diagnostic agents, drugs or nucleic acids into or onto the MB shell, molecular, multimodal and theranostic MBs can be generated. We here summarize recent advances in molecular, multimodal and theranostic US imaging, and introduce concepts how such advanced MB can be generated, applied and imaged. Examples are given for their use to image and treat oncological, cardiovascular and neurological diseases. Furthermore, we discuss for which therapeutic entities incorporation into (or conjugation to) MB is meaningful, and how US-mediated MB destruction can increase their extravasation, penetration, internalization and efficacy.

show abstract

“…A very innovative application of fluorescently labeled MB was presented by Yuan in 2009 [105]. Here, a fluorophore quencher-labelled MB system was used to measure external pressure.…”

Section: Multimodal Us Imagingmentioning

confidence: 99%

Recent advances in molecular, multimodal and theranostic ultrasound imaging

Kießling

Fokong

Bzyl

et al. 2014

Advanced Drug Delivery Reviews

182

151

View full text Add to dashboard Cite

show abstract

“…The resulting fluorescence emission intensity modulation has a theoretical efficiency calculated to be 100% 115. Fluorescence lifetime changes due to the modulated proximity of dyes on the microbubble would also be theoretically detectable 116. The expansion and contraction of the dye-loaded microbubble in response to focused ultrasound essentially makes the fluorescent microbubble “blink” at the driving frequency of the ultrasound 57,114.…”

Section: Microbubbles For Use In Imaging and Diagnosticsmentioning

confidence: 99%

Microbubble-mediated ultrasound therapy: a review of its potential in cancer treatment

Schutt¹,

Esener²

2013

DDDT

173

139

View full text Add to dashboard Cite

The inherently toxic nature of chemotherapy drugs is essential for them to kill cancer cells but is also the source of the detrimental side effects experienced by patients. One strategy to reduce these side effects is to limit the healthy tissue exposure by encapsulating the drugs in a vehicle that demonstrates a very low leak rate in circulation while simultaneously having the potential for rapid release once inside the tumor. Designing a vehicle with these two opposing properties is the major challenge in the field of drug delivery. A triggering event is required to change the vehicle from its stable circulating state to its unstable release state. A unique mechanical actuation type trigger is possible by harnessing the size changes that occur when microbubbles interact with ultrasound. These mechanical actuations can burst liposomes and cell membranes alike allowing for rapid drug release and facilitating delivery into nearby cells. The tight focusing ability of the ultrasound to just a few cubic millimeters allows for precise control over the tissue location where the microbubbles destabilize the vehicles. This allows the ultrasound to highlight the tumor tissue and cause rapid drug release from any carrier present. Different vehicle designs have been demonstrated from carrying drug on just the surface of the microbubble itself to encapsulating the microbubble along with the drug within a liposome. In the future, nanoparticles may extend the circulation half-life of these ultrasound triggerable drug-delivery vehicles by acting as nucleation sites of ultrasound-induced mechanical actuation. In addition to the drug delivery capability, the microbubble size changes can also be used to create imaging contrast agents that could allow the internal chemical environment of a tumor to be studied to help improve the diagnosis and detection of cancer. The ability to attain truly tumor-specific release from circulating drug-delivery vehicles is an exciting future prospect to reduce chemotherapy side effects while increasing drug effectiveness.

show abstract

“…Moreover, more sensitive UMF contrast agents based on fluorophore-labeled microbubbles have been developed to significantly improve the modulation efficiency through a quenching effect. 7,8,[12][13][14][15] One research group has designed a microbubble whose phospholipid shell was embedded with a type of lipophilic dye. 7,15 Recently, we developed a contrast agent by conjugating a type of NHS-ester-attached fluorophore on the surfaces of amine-functionalized microbubbles.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-modulated fluorescence based on donor-acceptor-labeled microbubbles

et al. 2015

Self Cite

View full text Add to dashboard Cite

Abstract. A fluorescence resonance energy transfer (FRET)-based microbubble contrast agent system was designed to experimentally demonstrate the concept of ultrasound-modulated fluorescence (UMF). Microbubbles were simultaneously labeled with donor and acceptor fluorophores on the surface to minimize self-quenching and maximize FRET. In response to ultrasound, the quenching efficiency was greatly modulated by changing the distance between the donor and acceptor molecules through microbubble size oscillations. Both donors and acceptors exhibited UMF on individual microbubbles. The UMF strength of the donor was more significant compared to that of the acceptor. Furthermore, the UMF of the donor was observed from a microbubble solution in a turbid media. This study exploits the feasibility of donor-acceptor labeled microbubbles as UMF contrast agents.

show abstract

Sensitivity of fluorophore-quencher labeled microbubbles to externally applied static pressure

Cited by 6 publications

References 54 publications

Recent advances in molecular, multimodal and theranostic ultrasound imaging

Recent advances in molecular, multimodal and theranostic ultrasound imaging

Microbubble-mediated ultrasound therapy: a review of its potential in cancer treatment

Ultrasound-modulated fluorescence based on donor-acceptor-labeled microbubbles

Contact Info

Product

Resources

About