Sensitivity of indirect immunofluorescence, substrate specificity, and immunoblotting in the diagnosis of pemphigus

Desmoglein 3-ELISA

“…[10][11][12][13] However, these methods are subjective, require an experienced examiner, and often fail to clearly distinguish …”

“…between PV, pemphigus foliaceus, and paraneoplastic pemphigus (PNP) 1,13 since these diseases share the lacelike squamous intercellular substance (ICS)-binding pattern. Although the differentiation is possible by immunoprecipitation and immunoblotting of epidermal extracts with patient serum samples, [13][14][15] both methods are time consuming and laborious.…”

Epistasis between DSG1 and HLA class II genes in pemphigus foliaceus

“…Anti-DSG1 (160 kDa) antibodies were detected by immunoblot using human skin extract as the antigen 26 in 15 patients (48.4%), a frequency similar to that usually reported. 27 Eighty-four healthy Caucasian volunteers were randomly selected among the representative panel of the French National Histocompatibility Center (Hopital Saint-Louis, Paris, France) to constitute the control population.…”

Serological Diagnosis of Autoimmune Blistering Diseases

“…Desmoglein 3'den (Dsg) zengin maymun özofagusu pemfigus vulgarisin (PV) IgG otoantikorlarını göstermede en sensitif substrattır (8). Dsg 1'den zengin gine domuzu özofagusu ise pemfigus foliaseus (PF) tanısında tercih edilen substrattır (9). Birden fazla substrat kullanımı ile tanısal sensitivite arttırılabilir.…”