Sensitivity of Three Selected Bacterial Species to Ozone

Abstract

The minimal lethal concentration of ozone in water was determined for three bacterial species: Escherichia coli, Bacillus cereus , and Bacillus megaterium . A contact period of 5 min was selected. The lethal threshold concentration for the cells of B. cereus was 0.12 mg/liter while that for E. coli and B. megaterium was 0.19 mg/liter. Low concentrations of ozone were ineffective when organic matter was present to interfere with the action of ozone on the bacterial cells. Also determined during the study was the sensitivity of spores of B. cereus and B. megaterium to ozone in water. The threshold concentration required to kill the spores of both species was 2.29 mg/liter. The cells and spores of these organisms exhibited the “all-or-none” die-away phenomenon normally associated with ozone treatment.

Ozone therapy as complementary treatment for surgical infection: case report.

“…Seemingly, it also shows superior results in the treatment of biofilms as compared to antibiotics. (17,19,20,21,22,23,24). Ozone therapy for infections seems to have similarities with hyperbaric oxygen treatment.…”

“…(16) Laboratory findings indicate ozone to have a high destructive potential on vegetative and planktonic forms of bacteria immersed in water and/or saline solution. (17) Results on its activity on biofilms are not so univocal however, ozone seems to have better destruction capacity then common treatment with antibiotics (18,19,20,21,22,23,24) Toxicological tests found that ozone, as applied to laboratory animals in acute and subacute doses, didn't show any tissue damage when studied either under electronic microscopy or histology. (25) We report a case of post-operative spine surgery infection treated successfully with intra-wound application of gaseous form of ozone.…”

Ozonated water antimicrobial efficacy in comparison to antimicrobial activity of different irrigant solutions against E. faecalis and E. coli

“…Treatment with ozone is a relatively new concept in dentistry. Ozone is a very powerful bactericide that can kill microorganisms effectively even at a concentration as low as 0.1ppm [16] . There are several known actions of ozone on human body, such as anti-microbial, immunostimulating, antihypoxic, analgesic, detoxicating, bioenergetic and biosynthetic (activation of the metabolism of carbohydrates, proteins & lipids) etc.…”

“…All the irrigation solutions at our disposable have their share of limitations and the search for an ideal root canal irrigant continues with the development of newer materials and methods [15] . Broadwater et al in 1973 reported that ozone at low concentration, 0.1 ppm, is sufficient to inactivate bacterial cells including their spores [16] . Ozone dissociates readily back into oxygen (O 2 ), thus liberating so called singlet oxygen (O 1 ), which is a strong oxidizing agent.…”

Ozone in Dentistry: A Review

“…22 Ozone in concentration of 0.1ppm is effective for inactivating the growth of the bacteria. 23 Ozone therapy increases oxygen perfusion to the tissues by the erythrocytes due to activation of 2,3diphosphoglycerate which in turn is activated due to increase in the red blood cell glycolysis rate. Ozone also stimulates production of ATP via Krebs cycle by enhancing oxidative carboxylation of pyruvate.…”