Sensory mother cell division is specifically affected in a Cyclin-A mutant of Drosophila melanogaster.

Ueda, Ryo; Togashi, Shin; Takahisa, Manabu; Tsurumura, S; Mikuni, M; Kondo, Kenji; Miyake, Tadashi

doi:10.1002/j.1460-2075.1992.tb05363.x

Cited by 13 publications

(8 citation statements)

References 29 publications

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“…We also note that the cln3 A‐10 mutation overlaps the region of the Cln3 HPD (Figure 1). These charged residues were not mutated in the original studies characterizing the HPD in mammalian cyclin A (Schafer et al , 1989; Ueda et al , 1992; Schulman et al , 1998; Cross and Jacobson, 2000). Similar to results seen with the Cln3‐hpm mutation of cln3 ‐A10 shows a defect in the cln − bck2 − viability assay at elevated temperatures.…”

Section: Discussionmentioning

confidence: 99%

Identification of novel and conserved functional and structural elements of the G1 cyclin Cln3 important for interactions with the CDK Cdc28 inSaccharomyces cerevisiae

Miller

Cross

Groeger

et al. 2005

Yeast

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Regions of the budding yeast G 1 cyclin Cln3 were characterized using mutational analysis and viability assays to identify functionally relevant and novel mutant alleles of CLN3. Cyclin proteins are conserved, and Cln3 contains a region with homology to the cyclin box, which is thought to mediate physical interactions with the cyclindependent kinase. CLN3 was found to have characteristics similar to the conserved cyclin fold found in higher eukaryotic cyclin boxes, which consist of five α-helices. Peptide linker sequences inserted within helices 1, 2, 3 and 5 resulted in a loss of Cln3 function, showing cyclin fold structure similar to that previously observed for the G 1 cyclin Cln2. A clustered-charge-to-alanine scan mutagenesis revealed two regions of Cln3 important for Cln3-dependent viability. The first region encompasses the conserved cyclin box. The second region is identified with alanine substitutions located well past the cyclin box, just prior to the C-terminal region of Cln3 important for protein stability. Cln3 with mutational changes in each of these regions are expressed at steady-state levels higher than wild-type Cln3, and show some defect in binding to Cdc28. The conserved hydrophobic patch domain (HPD) of cyclins is present within the first helix of the cyclin box. Alanine substitutions introduced into the HPD of Cln3 and Cln2 show functional defects while maintaining physical interaction with Cdc28 as measured by co-immunoprecipitation assay.

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Section: Discussionmentioning

confidence: 99%

Identification of novel and conserved functional and structural elements of the G1 cyclin Cln3 important for interactions with the CDK Cdc28 inSaccharomyces cerevisiae

Miller

Cross

Groeger

et al. 2005

Yeast

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“…Several lines of evidence suggested that rcal and cyclin A function in a common pathway: (1) the embryonic cell cycle phenotypes of cyclin A and rcal are similar; (2) rca I and cyclin A suppress both the meiotic and mitotic rux phenotypes; and (3) rcal is a dominant enhancer of a viable allele of cyclin A, cyclin A h~ (Ueda et al 1992; 98 data not shown). To gain insight into the function of rcal, we characterized it in more detail.…”

Section: Rcal Encodes a Novel Proteinmentioning

confidence: 99%

Control of G1 in the developing Drosophila eye: rca1 regulates Cyclin A.

et al. 1997

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“…Cyclin A is known to be required for SOP cell divisions and it has been proposed that proneural factors regulate its expression by binding to an element in its first intron [24]. Loss of one copy of the cyclin A gene resulted in suppression of Ch neuronal duplication in cato homozygote embryos (55% of segments with duplicated v'ch1 neurons, n = 42).…”

Section: Resultsmentioning

confidence: 99%

The function and regulation of the bHLH gene, cato, in Drosophila neurogenesis

Lage

Jarman

2010

BMC Dev Biol

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BackgroundbHLH transcription factors play many roles in neural development. cousin of atonal (cato) encodes one such factor that is expressed widely in the developing sensory nervous system of Drosophila. However, nothing definitive was known of its function owing to the lack of specific mutations.ResultsWe characterised the expression pattern of cato in detail using newly raised antibodies and GFP reporter gene constructs. Expression is predominantly in sensory lineages that depend on the atonal and amos proneural genes. In lineages that depend on the scute proneural gene, cato is expressed later and seems to be particularly associated with the type II neurons. Consistent with this, we find evidence that cato is a direct target gene of Atonal and Amos, but not of Scute. We generated two specific mutations of cato. Mutant embryos show several defects in chordotonal sensory lineages, most notably the duplication of the sensory neuron, which appears to be caused by an extra cell division. In addition, we show that cato is required to form the single chordotonal organ that persists in atonal mutant embryos.ConclusionsWe conclude that although widely expressed in the developing PNS, cato is expressed and regulated very differently in different sensory lineages. Mutant phenotypes correlate with cato's major expression in the chordotonal sensory lineage. In these cells, we propose that it plays roles in sense organ precursor maintenance and/or identity, and in controlling the number of cell divisions in the neuronal branch of the lineage arising from these precursors.

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Sensory mother cell division is specifically affected in a Cyclin-A mutant of Drosophila melanogaster.

Cited by 13 publications

References 29 publications

Identification of novel and conserved functional and structural elements of the G1 cyclin Cln3 important for interactions with the CDK Cdc28 inSaccharomyces cerevisiae

Identification of novel and conserved functional and structural elements of the G1 cyclin Cln3 important for interactions with the CDK Cdc28 inSaccharomyces cerevisiae

Control of G1 in the developing Drosophila eye: rca1 regulates Cyclin A.

The function and regulation of the bHLH gene, cato, in Drosophila neurogenesis

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