Separation and Detection of Certain ß-Lactam and Fluoroquinolone Antibiotic Drugs by Thin Layer Chromatography

Thangadurai, Subbiah; Shukla, Sudhir Kumar; Anjaneyulu, Y.

doi:10.2116/analsci.18.97

Cited by 28 publications

(26 citation statements)

References 16 publications

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“…Chromatogram was developed by loading 10 μl each fraction and running for half an hour. Spots on the plates were visualized in an iodine chamber [19,20].…”

Section: Thin Layer Chromatographymentioning

confidence: 99%

Purification of Secondary Metabolites From Soil Actinomycetes

Hemashenpagam¹

2011

Int J of Micr Res

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Abstract-A total of 25 different types of actinomycetes were isolated from the soils of Agricultural University. All the isolated actinomycetes were characterized and identified based on the morphological, biochemical, cultural characteristics .The isolates were belonged to Streptomyces, Micromonospora and Nocardia species. Optimization for the growth of all the isolates was carried out in batch culture. Strain was cultured on the basal medium with different carbon and nitrogen sources and their effect on the growth was studied. Both primary and secondary screening methods were used to screen actinomycetes for antibacterial activity. The result of the screening revealed that all the isolates were against bacterial culture. But the best strain was found to be Streptomyces sp as they showed broad spectrum activity with big zone of inhibition, after mutation, the strain Streptomyces sp showed increased antibacterial activity against all the tested human bacterial pathogens. The crude extract were further analysed by thin layer chromatography on silica plates. The Rf values in the TLC chromatograph further characterize that Streptomyces sp 2comes under aminoglycoside group, very much related to Streptomycin and the Streptomyces sp 3 comes under the antibiotic cephalexin. HPLC profile of the antimicrobial compounds of Streptomyces sp were performed by Rheodysne Column (C-18) up to 10 min at 220 nm. The peaks which had antimicrobial activity of Streptomyces sp1 was identified to be at 3.296. This is similar to oxohexaene antibiotic whereas, the antimicrobial compound of Streptomyces sp3 showed a retention time of 3.233 on HPLC, this peak was similar to cephalexin.

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“…Chromatogram was developed by loading 10 μl each fraction and running for half an hour. Spots on the plates were visualized in an iodine chamber [19,20].…”

Section: Thin Layer Chromatographymentioning

confidence: 99%

Purification of Secondary Metabolites From Soil Actinomycetes

Hemashenpagam¹

2011

Int J of Micr Res

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“…Treated plates transferred to TLC solvent system as mobile phase. After receiving of solvent front to end of plates, chromatograms observed on UV light at 256 nm (Thangadu et al, 2002).…”

Section: Pointing Running and Detectionmentioning

confidence: 99%

Management of Alternaria Leaf Blight of Bottle Gourd in Western Rajasthan, India

Maheshwari¹,

Choudhary²,

Sharma³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…OFL is a synthetic fluoroquinolone, and the current analytical methods devoted to determination OFL were capillary electrophoresis, 4 flow-injection spectrophotometry, 5 flow-injection chemiluminescence, 6,7 fluorescence spectrometry, 8 thin layer chromatography, 9 high-performance liquid chromatography (HPLC) 10 and HPLC-fluorometry. 11 However, these methods were limited in either complicated operation or sophisticated instrumentation or poor selectivity or poor limit of detection.…”

Section: Introductionmentioning

confidence: 99%

Determination of Antibacterial Ofloxacin in Human Serum Samples by a Resonance Light Scattering Technique with Alizarin Violet 3B

et al. 2009

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introductionOfloxacin (OFL), 9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-pierazinyl)-7-oxo-7H-pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid, Fig. 1, is one of the third-generation members of synthetic fluoroquinolone derivatives with antibacterial activity. This synthetic antibiotic is widely used in the treatment of urinary and respiratory tract infections and other infections, which are caught by Gram-positive and Gram-negative bacteria. 1 The bactericidal activity of OFL results from inhibiting enzyme DNA gyrase required for bacterial DNA replication.2 Because the OFL main escretion pathway is urinary, only trace amounts are found in plasma. 3 Therefore, great attention was paid to study the possibility of exposure to low levels of this medicine.Studies on determination of fluoroquinolones have attracted considerable interest in recent years. OFL is a synthetic fluoroquinolone, and the current analytical methods devoted to determination OFL were capillary electrophoresis, 4 flow-injection spectrophotometry, 5 flow-injection chemiluminescence, 6,7 fluorescence spectrometry, 8 thin layer chromatography, 9 high-performance liquid chromatography (HPLC) 10 and HPLC-fluorometry. 11 However, these methods were limited in either complicated operation or sophisticated instrumentation or poor selectivity or poor limit of detection. Therefore, the development of a reliable, rapid and accurate method for the determination of OFL is of great importance.Resonance light scattering (RLS) is a scattering-absorptionrescattering process that occurres when the wavelength of the incident beam exists in or close to the absorption band of the aggregation of particles. 12,13 Based on this theory, Pasternack et al. first established a new technique (RLS) to study the aggregations of porphyrins by using a common fluorescence spectrometry. [14][15][16] Huang et al. successfully applied this technique to the determination of nucleic acids at the nanogram level, and its detection limits were much lower than that of the fluorescence quenching method. 17,18 From then on, this simple and sensitive method has been greatly used for analytical purposes. 19,20 The RLS technique applied to the determination of nuclei acids, [21][22][23][24] proteins, 25-29 amino acid, 30 metal ions 31 and medicine 32 has already been reported. This method has proved to be promising based on the enhanced RLS intensity of the assay system. The RLS technique is becoming a novel analytical method characterized with high sensitivity, rapidity and simplicity.In this paper, the RLS spectra of alizarin violet 3B (AV3B) in the presence and absence of OFL are discussed. A reaction between AV3B and OFL occurred, and produced fine particles. The interaction between OFL and AV3B was also studied based on the absorption spectra, scanning electron micrograph and other analytical methods. The RLS signal was derived from fine particles of the formed complex, 13,14 and the intensity was enhanced by increasing the OFL in the system. The enhancement of the RLS intensity was p...

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Separation and Detection of Certain ß-Lactam and Fluoroquinolone Antibiotic Drugs by Thin Layer Chromatography

Cited by 28 publications

References 16 publications

Purification of Secondary Metabolites From Soil Actinomycetes

Purification of Secondary Metabolites From Soil Actinomycetes

Management of Alternaria Leaf Blight of Bottle Gourd in Western Rajasthan, India

Determination of Antibacterial Ofloxacin in Human Serum Samples by a Resonance Light Scattering Technique with Alizarin Violet 3B

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