Separation and identification of microplastics in marine organisms by TGA-FTIR-GC/MS: A case study of mussels from coastal China

Liu, Yi; Li, Ruojia; Yu, Jie; Ni, Fengli; Sheng, Yingfei; Scircle, Austin; Cizdziel, James V.; Zhou, Ying

doi:10.1016/j.envpol.2020.115946

Cited by 90 publications

(31 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A single chemical agent solution could not completely dissolve the tissue, often leaving undissolved residues at the bottom of the beaker. The mixture of H 2 O 2 and acid is more effective, but 10% KOH is the most effective for the dissolution of biological tissues without affecting MPs, with a recovery rate of over 97% (Liu et al, 2020). The same conclusion was also reached by comparing H 2 O 2 , proteinase K, trypsin, and KOH to dissolve bivalve tissues, using the following indicators: filterability, dissolution efficiency, and recyclability of MPs.…”

Section: Methods For Isolation and Characterization Of Microplastics In Mollusks Dissolutionmentioning

confidence: 64%

Microplastics in Mollusks: Research Progress, Current Contamination Status, Analysis Approaches, and Future Perspectives

et al. 2021

View full text Add to dashboard Cite

Plastic fragments < 5 mm, known as microplastics (MPs), are ubiquitously present in the marine environment. Research on MPs pollution has gradually shifted from field investigations to laboratory studies. With the rapid growth of plastic consumption and the prevalence of aquaculture products, studies on marine MPs have focused on key marine species, such as mollusks. This review summarizes the recent knowledge including 77 important relevant literatures (from 2010 to 2021) on MPs contamination in mollusks with the objectives of (1) elucidating the current status of MPs pollution levels in mollusks, (2) highlighting the main methods and techniques for separation, extraction, and identification of MPs in soft tissues of bivalves and (3) presenting the current research progress and future directions. The review visually presents some of the important results in graphic form, which shows that the most common polymer plastics in bivalves are polypropylene, polystyrene, and polyethylene, and the shapes were mainly fiber and threadiness, mollusks are more likely to feed smaller MPs, most of the MPs in bivalves are less than 500 μm, and the abundance of MPs in seawater and the abundance of MPs in mollusks have a positive relationship, etc. This review will provide a comprehensive reference for studies of microplastics in marine organisms and the ecological pollution, and also has scientific guiding significance in the research method.

show abstract

Section: Methods For Isolation and Characterization Of Microplastics In Mollusks Dissolutionmentioning

confidence: 64%

Microplastics in Mollusks: Research Progress, Current Contamination Status, Analysis Approaches, and Future Perspectives

et al. 2021

View full text Add to dashboard Cite

show abstract

“…3 and Online Resource 1 for time-lapse movie), which have become a world-wide concern. Since early reports about microplastic contamination in mussels (Browne et al 2008;von Moos et al 2012;Wegner et al 2012), an increasing number of papers have been published, mainly on species of Mytilus and Perna (Chae and An 2020; Gedik and Eryasar 2020; Kazour and Amara 2020; Piarulli and Airoldi 2020; Christoforou et al 2020;Cole et al 2020;Li et al 2020a;Stamataki et al2020;Wakkaf et al 2020;Webb et al 2020;Alnajar et al 2021;Cappello et al 2021;Cho et al 2021;Klasios et al 2021;Kumar et al 2021;Liu et al 2021;Perez et al 2021;Seuront et al 2021;Wang et al 2021a,b). Considering their distributions around cities and other human habitations, mussels are ideal organisms for monitoring microplastic pollution.…”

Section: Ingestion Of Microplasticsmentioning

confidence: 99%

Mussel biology: from the byssus to ecology and physiology, including microplastic ingestion and deep-sea adaptations

2021

View full text Add to dashboard Cite

Mussels are a group of bivalves that includes the dominant species of shallow-sea, freshwater, and deep-sea chemosynthetic ecosystems. Mussels cling to various solid underwater surfaces using a proteinaceous thread, called the byssus, which is central to their ecology, physiology, and evolution. Mussels cluster using their byssi to form “mussel beds,” thereby increasing their biomass per unit of habitat area, and also creating habitats for other organisms. Clustered mussels actively filter feed to obtain nutrients, but also ingest pollutants and suspended particles; thus, mussels are good subjects for pollution analyses, especially for microplastic pollution. The byssus also facilitates invasiveness, allowing mussels to hitchhike on ships, and to utilize other man-made structures, including quay walls and power plant inlets, which are less attractive to native species. Physiologically, mussels have adapted to environmental stressors associated with a sessile lifestyle. Osmotic adaptation is especially important for life in intertidal zones, and taurine is a major component of that adaptation. Taurine accumulation systems have also been modified to adapt to sulfide-rich environments near deep-sea hydrothermal vents. The byssus may have also enabled access to vent environments, allowing mussels to attach to “evolutionary stepping stones” and also to vent chimneys.

show abstract

“…In addition, thermal decomposition products can be precisely inspected by adding a mass spectrometry step. This helps to overcome the limitations of TGA-FTIR in terms of the polymer types that can be identified; consequently, various polymers (PE, PP, PS, PVC) can be distinguished [29]. In TGA thermal extraction and desorption (TED)-GC-MS, a sample can be qualitatively and quantitatively analyzed through TED-GC-MS after TGA analysis is performed.…”

Section: Complementary Use Of Thermal Methodsmentioning

confidence: 99%

Methods of Analyzing Microsized Plastics in the Environment

et al. 2021

View full text Add to dashboard Cite

Microplastics are found in various environments with the increasing use of plastics worldwide. Several methods have been developed for the sampling, extraction, purification, identification, and quantification of microplastics in complex environmental matrices. This study intends to summarize recent research trends on the subject. Large microplastic particles can be sorted manually and identified through chemical analysis; however, sample preparation for small microplastic analysis is usually more difficult. Microplastics are identified by evaluating the physical and chemical properties of plastic particles separated through extraction and washing steps from a mixture of inorganic and organic particles. This identification has a high risk of producing false-positive and false-negative results in the analysis of small microplastics. Currently, a combination of physical (e.g., microscopy), chemical (e.g., spectroscopy), and thermal analyses is widely used. We aim to summarize the best strategies for microplastic analysis by comparing the strengths and limitations of each identification method.

show abstract

Separation and identification of microplastics in marine organisms by TGA-FTIR-GC/MS: A case study of mussels from coastal China

Cited by 90 publications

References 46 publications

Microplastics in Mollusks: Research Progress, Current Contamination Status, Analysis Approaches, and Future Perspectives

Microplastics in Mollusks: Research Progress, Current Contamination Status, Analysis Approaches, and Future Perspectives

Mussel biology: from the byssus to ecology and physiology, including microplastic ingestion and deep-sea adaptations

Methods of Analyzing Microsized Plastics in the Environment

Contact Info

Product

Resources

About