Separation and identification of the insecticide “endosulfan” from biological materials

Coutselinis, A.; Kentarchou, P.; Boukis, D.

doi:10.1016/0300-9432(76)90139-4

Cited by 17 publications

(4 citation statements)

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“…Extraction was carried out for 4 h at 96°C. The sample extract from the soxhlet extractor was concentrated to reduce the volume of the solvent using a Kuderna-Danish Evaporative Assembly with a graduated evaporative concentrator tube (Coutselinis, Kentarchou, & Boukis, 1976 Manual of Analytical Methods for the Analysis of Pesticide Residues in Human and Environmental Samples, 1980).…”

Section: Tissue Treatmentmentioning

confidence: 99%

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Accumulation of Endosulfan in Wild Rat, Rattus norvegious as a Result of Application to Soya Bean in Mazoe (Zimbabwe)

Kuvarega

Taru

2007

Environ Monit Assess

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An assessment of levels of endosulfan and endosulfan sulphate in rats collected from a plot sprayed with endosulfan was carried out for a period of four months. The analysis was carried out in the livers, muscles and fatty tissues of the rats using Gas Chromatography. Samples were ground with sodium sulphate and aluminium oxide and the analyte soxhlet extracted with hexane. The extract was then cleaned by florisil column chromatography. Levels of endosulfan in rats were found to range in livers from 0.356 to 5.807 ppb, in muscles from 0.089 to 5.167 ppb and in fatty tissues from 0.083 to 4.785 ppb. Levels of endosulfan sulphate correlated well with those of endosulfan and ranged in livers from 0.00 to 3.96 ppb, in muscles from 0.00 to 3.37 ppb and in fatty tissue from 0.00 to 2.93 ppb. In the control rats samples, collected from an unsprayed area in the city of Harare (University of Zimbabwe), no endosulfan could be detected.

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Section: Tissue Treatmentmentioning

confidence: 99%

“…The collection tubes were removed from the Kuderna-Danish flasks and further cooled in cold water. Samples were then ready for analysis (Coutselinis et al, 1976(Coutselinis et al, , 1978 Manual of Analytical Methods for the Analysis of Pesticide Residues in Human and Environmental Samples, 1980).…”

Section: Florisil Fractionationmentioning

confidence: 99%

Accumulation of Endosulfan in Wild Rat, Rattus norvegious as a Result of Application to Soya Bean in Mazoe (Zimbabwe)

Kuvarega

Taru

2007

Environ Monit Assess

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“…Its chemical name is hexachloride-hexahydro-methanol-benzodioxathiepinoxide. According to a research [ 1 ], it is a cyclic sulphurous acid ester with the chemical formula C 9 H 6 O 3 Cl 6 S and the molecular weight of 407 g/mole. The term “pesticide” refers to a substance that is often employed in agriculture or for the protection of public health and is made up of a combination of several chemicals.…”

Section: Introductionmentioning

confidence: 99%

Endosulfan induces reproductive & genotoxic effect in male and female Swiss albino mice

Priya,

Kumar,

Ali

et al. 2024

Lab Anim Res

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Background Toxicity by pesticide has become a global health issue and leaves a harmful impact on human health via various ways. The people exposed to pesticides in the rural population get affected by the harmful effects of it as they enter the human body system through skin, inhalation, oral administration, food chain and many more ways. The present work is designed to study the toxic effect of endosulfan in male (n=30) and female (n=30) Swiss albino mice. Endosulfan was administered by oral gavage (oral administration) method, at the dose of 3.5 mg/Kg body weight daily for period of 3 weeks, 5 weeks and 7 weeks. After the completion of the treatment, the mice were sacrificed and their ovary and testis tissues were dissected out to check the degeneration. The blood was collected for karyotyping, biochemical and hormonal analysis of pesticide induced genotoxicity. After 7 weeks of administration with Endosulfan, various abnormalities were observed in male and female mice. Results Treatment with endosulfan at the dose of 3.5 mg/Kg body weight caused a higher degree of degeneration in the reproductive organ of Swiss albino mice . Treatment by this pesticide generated degeneration in long duration of dosage for 3,5 and 7 weeks. Ovaries of endosulfan administered groups showed degenerated germinal epithelium, Graffian follicles and corpus luteum. In testis of endosulfan treated mice, microscopic examination showed that there is significant damage and reduction in the tissue of seminiferous tubules and primordial germ cells. High degree of degeneration caused the disarrangement and deformation of spermatogonia with the decrease in the number of Sertoli cells. Biochemical and hormonal properties was also affected by endosulfan treatment. There was significant 5 folds decrease in the testosterone value of endosulfan in 7 weeks treated mice in comparison to control (p < 0.0001) and similarly there was significant elevation in the estrogen levels found in 7th week endosulfan treated mice. It also influenced the level of free radicals as there was significant decrease (p < 0.0001) in the value in catalase levels in 7 weeks endosulfan treated male and female mice, while significant (p < 0.0001) increase in the values of lipid peroxidation levels as 8 folds and 10 folds in 7 weeks endosulfan treated male and female Swiss albino mice respectively. This study hence speculates that the endosulfan exposed population are at the risk of reproductive health hazards. Conclusions The present study thus concludes that, endosulfan after 7 weeks of exposure caused significant reproductive damage to both male and female Swiss albino mice groups. Moreover, the karyotyping study also correlated the genotoxic damage in the mice.

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“…It is a cyclic sulphurous acid ester with a molecular formula C 9 H 6 O 3 Cl 6 S and molecular weight 407. Despite its life-threatening toxic effects, endosulfan continues to be one of the most widely used agricultural pesticides, largely in the developing countries, due to its high efficacy, low cost and environmental stability (Coutselinis et al, 1976;Yadla et al, 2013). It is easily absorbed in the gastrointestinal tract, lungs and skin and exposure through various routes and is very hazardous.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Withania somnifera on lipid profile of endosulfan induced toxicity in Swiss albino mice

Jha

Paul

2020

JANS

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India is an agrarian country with crops cultivated at a huge scale. Pesticides in recent times have caused serious health hazards in the population which are widely used by the farmers for the better yield of crops. Endosulfan is an organochlorine pesticide, which is widely used by the farmers. But, in the present times, it has caused serious health hazards in the exposed population causing various diseases, including cancer. Hence, the present study on animal aims to observe the protective effect of Withania somnifera against endosulfan induced toxicity in Swiss albino mice. Endosulfan at the dose of 3mg/Kg body weight per day was administered orally to Swiss albino mice for 4 weeks. Then after, W. somnifera at the dose of 1000 mg/Kg b.w. was orally administered for 4 weeks. Mice were sacrificed after the completion of the entire treatment. After dissection, the blood samples were collected for biochemical assay, especially for lipid profile analysis. The lipid profile study showed inclination in the Total cholesterol level (117±6.686 mg/dl), Cholesterol (LDL) (78.83±4.151mg/dl), level and Triglycerides level (60.83±2.613mg/dl), while declination in Cholesterol (HDL) (13.50±1.33mg/dl), level after Endosulfan exposure. But, upon W. somnifera treatment to the endosulfan treated group showed significant (p<0.001) normalisation in the lipid profile levels. Therefore, it was concluded that W. somnifera played a vital role to control the endosulfan induced toxicity.

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Separation and identification of the insecticide “endosulfan” from biological materials

Cited by 17 publications

References 1 publication

Accumulation of Endosulfan in Wild Rat, Rattus norvegious as a Result of Application to Soya Bean in Mazoe (Zimbabwe)

Accumulation of Endosulfan in Wild Rat, Rattus norvegious as a Result of Application to Soya Bean in Mazoe (Zimbabwe)

Endosulfan induces reproductive & genotoxic effect in male and female Swiss albino mice

Efficacy of Withania somnifera on lipid profile of endosulfan induced toxicity in Swiss albino mice

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