Separation and Purification of Polyhydroxyalkanoates from Newly Isolated<i>Comamonas</i>sp. EB172 by Simple Digestion with Sodium Hydroxide

Mohammadi, Mitra; Hassan, Mohd Ali; Shirai, Yoshihito; Man, Hasfalina Che; Ariffin, Hidayah; Yee, Lian-Ngit; Mumtaz, Tabassum; Chong, Mei-Ling; Phang, Lai Yee

doi:10.1080/01496395.2011.615788

Cited by 29 publications

(15 citation statements)

References 22 publications

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“…EB172 were carried out according to the method as reported by Mumtaz et al (2009) while recombinant C. necator containing PHA was kindly provided by Dr. Kumar Sudesh (Universiti Sains Malaysia, Penang, Malaysia). The preparation of biomass containing PHA prior to recovery process was described elsewhere (Mohammadi et al, 2011a(Mohammadi et al, , 2011b.…”

Section: Pha-containing Biomassmentioning

confidence: 99%

“…PHA purity, recovery yield, protein concentration and polymer molecular weight were analyzed using the protocols as reported previously (Mohammadi et al, 2011a(Mohammadi et al, , 2011b. The morphology of PHA granules before and after the recovery was observed under scanning electron microscopy (JEOL JSM-6400 SEM).…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…The PHA recovery efficiency in respect to PHA content was examined whereby PHA content of 30 and 50% were used. The PHA recovery processes by NaOH and water were performed as described previously (Mohammadi et al, 2011a(Mohammadi et al, , 2011c. Purification steps were accomplished by using ethanol and water.…”

Section: Pha Recovery Using Naoh and Watermentioning

confidence: 99%

“…EB172 and recombinant C. necator using mild alkaline and water recovery methods as cleaner and environmentally more benign process based on several process parameters, i.e. NaOH concentration, time and temperature (Mohammadi et al, 2011a(Mohammadi et al, , 2011b(Mohammadi et al, , 2011c. However, the halogen-free methods chosen in this study require further adjustment, depending on both significant process parameters and external factors influencing their performance to make the process suitable for polymer recovery at the industrial scale.…”

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill

Mohammadi

Hassan

Phang

et al. 2012

Journal of Cleaner Production

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Section: Pha-containing Biomassmentioning

confidence: 99%

Section: Analytical Proceduresmentioning

confidence: 99%

Section: Pha Recovery Using Naoh and Watermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill

Mohammadi

Hassan

Phang

et al. 2012

Journal of Cleaner Production

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“…EB172 [10][11][12][13][14]. While developing a suitable fermentation strategy to feed mixed acids in its original form (keeping identical ratio as obtained), our focus was also to develop a non-halogenated PHA recovery system, at the end of fed-batch fermentation [10,15,16]. However, Comamonas sp.…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxyalkanoate Synthesis by Recombinant Escherichia coli JM109 Expressing PHA Biosynthesis Genes from Comamonas sp. EB172

Yee¹,

Mumtaz²,

Mohammadi³

et al. 2012

J Microb Biochem Technol

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Recombinant Escherichia coli JM109 harbouring the polyhydroxyalkanoate (PHA) biosynthesis gene (phaCABco) of Comamonas sp. EB172, an acid tolerant microbe, was examined for the production of PHAs from various carbon sources. The study demonstrated that the recombinant E. coli JM109 had the potential to utilize both sugar-and acid-based carbon sources, for the biosynthesis of both poly(3-hydroxybutyrate) P(3HB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) copolymers. In the shake flask experiments, the strain was capable of producing P (3HB-co-3HV) copolymer from mixed organic acids, and higher productivities were obtained using glucose compared to mixed acids. However, PHA accumulation was found to be similar, regardless of the carbon source used. Nitrogen supplementation in the medium was found to improve the cell dry weight, but negatively affected the 3HV formation in copolymer production. Maximum 3HV monomer (3 mol%) was obtained with C/N 42.1, using mixed acids as the carbon source. In the 2L bioreactor, the productivity and yield based on substrate utilization coefficient were found to be 0.16 g PHA/(L.h) and 0.41 g PHA/g substrate under C/N around 75, using 20 g/L glucose and 0.5 g/L ammonium sulphate, respectively. The polymer produced by the recombinant strain had molecular weight in the range of 8.5 x 105 to 1.4 x 106 Da. Overall, the ability of the recombinant E. coli JM109 to utilize both glucose and mixed acids, has widened its substrate selection for fermentation, including the opportunity to use renewable biomass.

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Sustainable Opportunities in the Downstream Processing of the Intracellular Biopolymer Polyhydroxyalkanoate

Bhat,

Thivaharan,

Divyashree

2023

ChemBioEng Reviews

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Biopolymers have gained popularity as an alternative product to traditional plastics due to environmental concerns. Polyhydroxyalkanoates (PHAs), one of the most well‐known forms of biopolymers, are among the many that have been discovered so far. PHA has a wide range of applications, is biodegradable and compatible with living things, but its expensive extraction and production make it difficult to compete with traditional plastics. Solvents are the most popular extraction technique but have serious economic and environmental downsides. The “green method of extraction” has become a cutting‐edge remedy for addressing the shortcomings of downstream processing. However, developing the extraction technique for affordable and environmentally friendly biopolymer production is a difficult study topic. To make it easier to choose green recovery techniques for future study, this review combines the benefits and drawbacks of the numerous PHA recovery methods that are already in use. Also, advanced green downstream methods are scrutinized that undoubtedly makes PHA replace conventional plastics and reach a market globally promoting sustainability.

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Separation and Purification of Polyhydroxyalkanoates from Newly IsolatedComamonassp. EB172 by Simple Digestion with Sodium Hydroxide

Cited by 29 publications

References 22 publications

Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill

Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill

Polyhydroxyalkanoate Synthesis by Recombinant Escherichia coli JM109 Expressing PHA Biosynthesis Genes from Comamonas sp. EB172

Sustainable Opportunities in the Downstream Processing of the Intracellular Biopolymer Polyhydroxyalkanoate

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