Separation and quantification of phenolic metabolites using thin-layer chromatography followed by ultraviolet–visible spectroscopy: Urinary biomarkers of benzene exposure

Anand, Astitva; Dahiya, M. S.; Agarwal, Rakhi

doi:10.1556/1006.2017.30.6.3

Cited by 1 publication

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“…Urine sample (0.5 mL) was taken into 4 mL glass vial and digested using 50 µL of concentrated hydrochloric acid. Liquid-liquid micro extraction (LLME) was done using 1.5 mL ethyl acetate as extraction solvent [18]. Extraction was done by vortexing the urine and extraction solvent mixture for 2 min.…”

Section: Methodsmentioning

confidence: 99%

“…Till date, various techniques have been used for analysis of benzene metabolites in human blood, urine and tissues [17]. Recently, we have reported thin layer chromatography method for separation and further quantitation by UV-visible spectroscopy for phenolic benzene metabolites [18]. Present work includes the detection of benzene metabolites using UPLC-PDA-FLD and then their mass spectrometric confirmation with IPS-GC-MS techniques for diagnosis and clinical investigation purposes in benzene poisoning cases.…”

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confidence: 99%

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Differentiation of Immediate and Delayed Metabolic Biomarkers: Qualitative Assessment of Benzene Exposure in Cigarette Smokers and Petrol Station Workers

Anand¹,

Agarwal²

2018

Asian J. Chem.

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Benzene is known an omnipresent pollutant, a common industrial chemical and a prominent constituent of gasoline, tobacco smoke and engine emission [1,2]. Human population exposes to benzene includes petrochemical workers, petrol station attendants, glue workers, rubber industry workers and smokers etc. [1,3]. In 1987, Classification of International Agency for Research on Cancer (IARC) depicts benzene as a group-I carcinogen [4]. Exposure to benzene results in aplastic anemia, dysfunction of the immune system and leukemia [5]. Benzene is naturally occurring component of petroleum products and present in gasoline (petrol), thus petrol station workers are most likely to be affected from benzene toxicity [6]. Rahman et al. [7] and Barbera et al. [8] have reported benzene poisoning when benzene is stupefied with ethyl alcohol in medico-legal poisoning like accidental and homicidal cases.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%