Separation of amino acids, their derivatives and enantiomers by impregnated TLC

Abstract

The present state of TLC with respect to separation of amino acids, their different derivatives and their enantiomers by the technique of impregnation is discussed. The main approaches to impregnation viz. mixing of a suitable reagent with the adsorbent prior to plate-making, immersion of the untreated plate in the solution of impregnating reagent prior to development, and modification of the adsorbent, have been identified and discussed for each class of these compounds. The role of impregnation in resolving enantiomers or in improving the separation of mixtures of amino acids or their derivatives in terms of ion pairing, complex formation, ligand exchange or other steric interactions has been elaborated in each category.

Divergent Roles in Arabidopsis thaliana Development and Defense of Two Homologous Genes, ABERRANT GROWTH AND DEATH2 and AGD2-LIKE DEFENSE RESPONSE PROTEIN1, Encoding Novel Aminotransferases

“…Baker, Phillipsburg, NJ) and developed with n-butyl alcohol:acetic acid:water (3:1:1, v/v). The chromatogram, dried at 608C and cooled to room temperature, was soaked in freshly prepared ninhydrin solution (0.2% in acetone) for 1 min (Bhushan and Martens, 2001). To quantify the signals, the National Institutes of Health Image J program was used (http://rsb.info.nih.gov/ij/).…”

Direct enantiomeric resolution of (±)‐atenolol, (±)‐metoprolol, and (±)‐propranolol by impregnated TLC using L‐aspartic acid as chiral selector

“…Direct resolution of enantiomers by impregnated TLC has been reviewed by Bhushan and Martens (1997, 2001.…”

Enantioresolution of Amino Acids: A Decade’s Perspective, Prospects and Challenges

“…The role of impregnation in resolving enantiomers or in improving the separation of mixtures of AAs or their derivatives in terms of ion pairing, complex formation, ligand exchange or other steric interactions has been reviewed by Bhushan and Martens [43]. Nevertheless, the chiral selector must be a pure enantiomer and should provide interactions such as π-π, n-π, hydrogen bonding, ion-ion and ion-dipole interactions with at least at three points of one enantiomer (of the analyte) and at two points of the other enantiomer of the chiral molecule (the analyte).…”