Separation of tetra‐, penta‐, and hexaacyl triglycerides by high performance liquid chromatography

Abstract

Synthetic triglycerides with more than three acyl groups were prepared by forming estolides from triglycerides containing one, two, or three monohydroxy fatty acyl moieties. These tetra‐, penta‐, and hexaacyl triglycerides were examined by high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). HPLC separation by the number of acyl groups was obtained with both conventional phase and reverse phase columns. In all systems, triglycerides were eluted first, followed in sequence by tetra‐, penta‐, and hexaacyl triglycerides. Within each glyceride class, further separation occurred due to variations in chain length and degree of unsaturation among the component acids. TLC migration on silica gel was found to be a function of the number of acyl groups and the length of component acid chains.

Estolide triglycerides ofTrewia nudiflora seed oil

“…Preparative TLC of estolide oils by multiple development with relatively nonpolar solvent systems was used by Morris and Ha// (20) to separate the estolide-containing glycerides of ergot oil from Claviceps purpurea. Payne-Wahl et al (21) used multiple-development with benzene in the separation of synthetic multiacyl triglycerides. By applying this technique, we were able to obtain a series of at least 12 discrete fractions (Table 1).…”

Hydroxy acids and estolide triglycerides ofHeliophila amplexicaulis L.f. Seed oil

“…Detection of components was made by differential refractometry. A standard methyl ester estolide was made by reacting methyl ricinoleate with palmitoyl chloride as reported by Payne-Wahl et al (6). Mass spectra were obtained at 70 eV using the gas chromatographic (GC) inlet on the Bendix 2625-Dupont 21-492-1 gas chromatography-mass spectrometer (GC-MS) system.…”

High performance reversed‐phase chromatography of natural triglyceride mixtures

“…The introduction of high performance reversed-phase chromatography (HPRC) has revolutionalized the field of lipid separation and analysis (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33). Recently the use of HPRC in triglyceride separation and analysis has increased (24)(25)(26)(27)(28)(29)(30)(31)(32)(33). Pei,et al (27) used Vydac reversed-phase packing to separate triglycerides using methanol:water as a mobile phase.…”

“…Pei,et al (27) used Vydac reversed-phase packing to separate triglycerides using methanol:water as a mobile phase. Columns packed with ~-Bondapac C-18 have been utilized in the separation and analysis of triglyeerides using different mobile phases and the refractometer as a detector (28)(29)(30)(31). Parris (32) first separated triglyeerides on a Zorbax ODS column using nonaqueous solvent mixtures of methylene chloride, tetrahydrofuran and aeetonitrile as mobile phases and detected the separated components with an infrared detector.…”