Separation procedures for the pharmacologically active components of rhubarb

Zhang, Haixia; Liu, Mancang

doi:10.1016/j.jchromb.2004.08.010

Cited by 36 publications

(23 citation statements)

References 27 publications

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“…According to the methods of previous reports [17][18][19] , RPE was fractionated by RP-HPLC using the Shimadzu 10A-VP HPLC system. IR spectra were measured with a Perkin-Elmer 2000 FT-IR spectrophotometer.…”

Section: Isolation Of Anthraquinones From Rpementioning

confidence: 99%

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Identification of Natural Compounds with Anti-Hepatitis B Virus Activity from <i>Rheum palmatum</i> L. Ethanol Extract

Sun

et al. 2007

Chemotherapy

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Background: Hepatitis B virus (HBV) infection is a severe health problem in the world; however, there is still no satisfactory therapeutic strategy for the HBV infection. In search for new anti-HBV agents with higher efficiency and less side effects, the anti-HBV activities of traditional Chinese medicine Rheum palmatum L. ethanol extract (RPE) and isolated anthraquinones were evaluated. Methods: The anti-HBV activities of RPE and isolated anthraquinones were demonstrated in a stable HBV-producing cell line HepG2 2.2.15 by using real-time polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA) and Southern blot analysis. Results: RPE could inhibit HBV-DNA production and HBsAg expression in a dose-dependent manner. The concentration of 50% HBV-DNA inhibition (IC₅₀) of RPE was calculated at 212.36 ± 11 µg/ml. Six anthraquinones were isolated from RPE by using RP-HPLC. Five free anthraquinones showed weakly or slightly inhibitory activities against HBV. The only combined anthraquinone chrysophanol 8-O-β-D-glucoside exhibited significant activity against HBV DNA production and antigens expression with an IC₅₀ value of 36.98 ± 2.28 µg/ml on HBV DNA inhibition. Endogenous HBV DNA polymerase activity assay indicated that chrysophanol 8-O-β-D-glucoside might be a potential inhibitor of the HBV DNA polymerase. Conclusions: The results suggested that RPE could effectively inhibit HBV. The combined anthraquinone chrysophanol 8-O-β-D-glucoside is the major active compound in RPE and could be a promising candidate for the development of new anti-HBV drugs in the treatment of HBV infection.

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Section: Isolation Of Anthraquinones From Rpementioning

confidence: 99%

“…It would be very important to separate the mixture into its major individual components and identify the effective anti-HBV compound(s). According to the methods of previous reports [17][18][19] , RPE was fractionated by RP-HPLC using the Shimadzu 10A-VP HPLC system. By comparing the melting-point, …”

Section: Isolation Of Anthraquinones From Rpementioning

confidence: 99%

Identification of Natural Compounds with Anti-Hepatitis B Virus Activity from <i>Rheum palmatum</i> L. Ethanol Extract

Sun

et al. 2007

Chemotherapy

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“…Our research group has investigated the distribution and genetic variation of the section Palmata (Wang et al, 2010(Wang et al, , 2012a, and many previous studies on this section have focused on components analysis (Zheng and Zhang, 1993;Zhang and Liu, 2004;Ye et al, 2007) and pharmacological properties (Xiao et al, 1984;Yu et al, 2005;Tseng et al, 2006). However, the chromosome number and karyotype of this section are not yet known.…”

Section: Introductionmentioning

confidence: 99%

Karyotype analysis of Rheum palmatum

Jia

Fan

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Rheum palmatum, one of the source plants of the traditional Chinese medicine rhubarb, is anendemic and endangered species. To our knowledge, this is the first report on the chromosome number and karyotype of this species. Sectioning combined with micrography was used to analyze the karyotype. The following results were obtained: R. palmatum had a stable chromosome number 2n = 22; the basic number of chromosomes was 11; karyotype formula is 2n = 22 = 20 metacentric + 2 submetacentric, belonging to Stebbins' 1A type; and karyotype asymmetry index was 55.39%. The present study showed that R. palmatum has a primitive type of karyotype.

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“…Therefore, a simple, rapid, and accurate method for the analysis of bioactive compounds in rhubarb is necessary for the quality control of crude rhubarb drugs and their pharmaceutical preparations. Qualitative and quantitative analyses of rhubarbs have been extensively pursued [11][12][13][14]. However, most of these investigations focus on the determination of anthraquinones by TLC, HPLC, and HPCE.…”

mentioning

confidence: 99%

Analysis of phenolic compounds in rhubarbs using liquid chromatography coupled with electrospray ionization mass spectrometry

Han

Chen

et al. 2007

J. Am. Soc. Mass Spectrom.

231

157

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Rhubarb is an important herbal medicine for the treatment of constipation, inflammation, and cancer. In this study, a facile method based on liquid chromatography coupled with electrospray ionization tandem mass spectrometry has been established for the analysis of bioactive phenolic compounds in rhubarbs. From six rhubarb species, official (Rheum officinale, R. palmatum, and R. tanguticum) and unofficial (R. franzenbachii, R. hotaoense, and R. emodi), a total of 107 phenolic compounds were identified or tentatively characterized based on their mass spectra. These compounds include sennosides, anthraquinones, stilbenes, glucose gallates, naphthalenes, and catechins. Ion chromatograms for the identified compounds of different rhubarbs were then compared. Consistent with previous reports, sennosides and rhein were only detected in official rhubarbs. Unexpectedly, we found that R. officinale contained very different phenolic compounds from the other two official species. Sennoside A, which has been considered as the major purgative component of rhubarb, was only detected in R. officinale, while its close isomers were observed in R. palmatum and R. tanguticum. In addition, the predominant anthraquinone glycosides in R. officinale were found to be rhein 8-O-glucoside and emodin 1-O-glucoside, whereas those in R. palmatum and R. tanguticum were rhein 1-O-glucoside and emodin 8-O-glucoside. Stilbenes, which are the major constituents of unofficial rhubarbs, were also different among the species. Our results clarify the chemical composition of rhubarbs comprehensively for the first time. Due to the significant differences in chemical components of rhubarbs, we suggest that different Rheum species be used separately in clinical practice. (J Am Soc Mass Spectrom 2007, 18, 82-91)

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Separation procedures for the pharmacologically active components of rhubarb

Cited by 36 publications

References 27 publications

Identification of Natural Compounds with Anti-Hepatitis B Virus Activity from <i>Rheum palmatum</i> L. Ethanol Extract

Identification of Natural Compounds with Anti-Hepatitis B Virus Activity from <i>Rheum palmatum</i> L. Ethanol Extract

Karyotype analysis of Rheum palmatum

Analysis of phenolic compounds in rhubarbs using liquid chromatography coupled with electrospray ionization mass spectrometry

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