Sequence Analysis and Androgen Regulation of MHG07 (Male Harderian Gland) mRNA in Male Hamster Harderian Gland

Hormonal regulation and characterisation of the aldehyde oxidase-like gene of hamster Harderian gland

“…These findings seem to be closely related to the expression of an androgen receptor [12,16,17]. Esposito et al [9] found that a cDNA clone named MHG07 is expressed into male HG and in the liver of both sex. Castration leads to the disappearance of MHG07 mRNA within 4 days, and testosterone treatment restore its expression.…”

“…In primary cultures of male hamster HG, androgens increase the MHG07 expression and such an effect is blocked by both flutamide and cycloheximide. It is expressed in male HG only and male and female liver [9]. Furthermore, the expression of MHG07 mRNA is paralleled by ARmRNA expression in primary cultures [9].…”

“…FHG22 mRNA is expressed in female gland only and both in vivo and in vitro studies have shown that estrogen regulate its expression [8]. In male has been sequenced the clone MHG07 [9]. This clone has not significant homologies with other polypeptides/genes.…”

“…Hybridisation was performed with a 1.17-kb BamHI-XbaI fragment from the male hamster MHG07 cDNA (pcDNAII) [9] or with a 2.1-kb BamHI fragment from the human cDNA of ␤-actin (pHFbA-1) [13]). Probes were labelled with [␣ 32 P]dCTP by random priming to a specific activity of about 5 × 10 8 cpm/g [14].…”

“…It is expressed in male HG only and male and female liver [9]. Furthermore, the expression of MHG07 mRNA is paralleled by ARmRNA expression in primary cultures [9]. In understanding the molecular mechanisms involved into regulation of gene expression we have undertaken this study on the regulation of the expression of thismale specific gene by other steroids.…”