Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae

“…Nodaviruses have been detected previously in human stool (13) and are semipermissive of replication in mammalian tissues (4,12). Dicistroviruses have been shown to replicate and be pathogenic in insects (10,37). The internal ribosomal entry site between the two cistronic segments can act as a powerful promoter in mammalian cells (28).…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Analyses of Viruses in Stool Samples from Children with Acute Flaccid Paralysis

Victoria

Kapoor

et al. 2009

J Virol

343

345

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We analyzed viral nucleic acids in stool samples collected from 35 South Asian children with nonpolio acute flaccid paralysis (AFP). Sequence-independent reverse transcription and PCR amplification of capsid-protected, nuclease-resistant viral nucleic acids were followed by DNA sequencing and sequence similarity searches. Limited Sanger sequencing (35 to 240 subclones per sample) identified an average of 1.4 distinct eukaryotic viruses per sample, while pyrosequencing yielded 2.6 viruses per sample. In addition to bacteriophage and plant viruses, we detected known enteric viruses, including rotavirus, adenovirus, picobirnavirus, and human enterovirus species A (HEV-A) to HEV-C, as well as numerous other members of the Picornaviridae family, including parechovirus, Aichi virus, rhinovirus, and human cardiovirus. The viruses with the most divergent sequences relative to those of previously reported viruses included members of a novel Picornaviridae genus and four new viral species (members of the Dicistroviridae, Nodaviridae, and Circoviridae families and the Bocavirus genus). Samples from six healthy contacts of AFP patients were similarly analyzed and also contained numerous viruses, particularly HEV-C, including a potentially novel Enterovirus genotype. Determining the prevalences and pathogenicities of the novel genotypes, species, genera, and potential new viral families identified in this study in different demographic groups will require further studies with different demographic and patient groups, now facilitated by knowledge of these viral genomes.

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“…These include two DNA viruses, Myzus persicae densovirus (MpDNV) (van Munster et al, 2003b) and Dysaphis plantaginea densovirus (DplDNV) (Ryabov et al, 2009), and five RNA viruses, aphid lethal paralysis virus (ALPV) (van Munster et al, 2002), Rhopalosiphum padi virus (Moon et al, 1998), Acyrthosiphon pisum virus (van der Wilk et al, 1997), Brevicoryne brassicae virus (Ryabov, 2007) and rosy apple aphid virus (RAAV) (Ryabov et al, 2009). While these aphid DNA viruses are around 5 kb in size the RNA viruses are larger, around 10 kb in size.…”

Section: Introductionmentioning

confidence: 99%

A novel virus from Macrosiphum euphorbiae with similarities to members of the family Flaviviridae

Teixeira

Sela

et al. 2016

Journal of General Virology

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A virus with a large genome was identified in the transcriptome of the potato aphid (Macrosiphum euphorbiae) and was named Macrosiphum euphorbiae virus 1 (MeV-1). The MeV-1 genome is 22 780 nt in size, including 39 and 59 non-coding regions, with a single large ORF encoding a putative polyprotein of 7333 aa. The C-terminal region of the predicted MeV-1 polyprotein contained sequences with similarities to helicase, methyltransferase and RNA-dependent RNA polymerase (RdRp) motifs, while the N-terminal region lacked any motifs including structural proteins. Phylogenetic analysis of the helicase placed MeV-1 close to pestiviruses, while the RdRp region placed it close to pestiviruses and flaviviruses, suggesting MeV-1 has a positive-polarity ssRNA genome and is a member of the family Flaviviridae. Since the MeV-1 genome is predicted to contain a methyltransferase, a gene present typically in flaviviruses but not pestiviruses, MeV-1 is likely a member of the genus Flavivirus. MeV-1 was present in nymphal and adult stages of the aphid, aphid saliva and plant tissues fed upon by aphids. However, the virus was unable to multiply and spread in tomato plants. In addition, dsRNA, the replication intermediate of RNA viruses, was isolated from virus-infected M. euphorbiae and not from tomato plants infested with the aphid. Furthermore, nymphs laid without exposure to infected plants harboured the virus, indicating that MeV-1 is an aphid-infecting virus likely transmitted transovarially. The virus was present in M. euphorbiae populations from Europe but not from North America and was absent in all other aphid species tested.

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Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae

Cited by 68 publications

References 40 publications

Characterization of Cell Lines Developed From the Glassy-Winged Sharpshooter, Homalodisca Coagulata (Hemiptera: Cicadellidae)

Characterization of Cell Lines Developed From the Glassy-Winged Sharpshooter, Homalodisca Coagulata (Hemiptera: Cicadellidae)

Metagenomic Analyses of Viruses in Stool Samples from Children with Acute Flaccid Paralysis

A novel virus from Macrosiphum euphorbiae with similarities to members of the family Flaviviridae

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