Sequence Analysis of a New Short Tandem Repeat Locus D17S2266E Located in the Human Growth Hormone Gene Cluster HumGH@*

Abstract

D17S2266E is a new, variable genetic marker exhibiting polymorphism of the number of repeats of four- and two-nucleotide motifs. This study, carried out on a group of 250 unrelated persons from various regions of Poland, revealed the presence of 24 different alleles ranging in size from 232 to 290 base pairs. Analysis of the sequenced fragments demonstrated that the alleles consisted of two flanking regions and two variable blocks that were separated by a consensus sequence. There were (AAAG)(5)(AG)(1)(AAAG)(3-4) repeats in the first block, and [(AAAG)(2)(AG)(1)](0-1)[(AAAG)(6)(AG)(1)](0-1)(AAAG)(n) repeats in the second block. On the basis of the allele frequencies in the population, we were able to do biostatistical calculations, which gave the following results: expected heterozygosity 0.8947 +/- 0.0137, power of discrimination 0.9793, polymorphism information content 0.8837, probability of exclusion (PE) 0.7859, PE for motherless cases 0.6473, and an average paternity index of 4.7470. These biostatistical parameters show that the marker D17S2266E can find a wide range of applications in forensic testing.

Forensic Characterization of Four New Bovine Tri-nucleotide Microsatellite Markers in Korean Cattle (Hanwoo)

“…The other sequenced alleles (23.2, 36.2, and 44.2) were compound repeat structures, which were interrupted by a internal consensus sequence; (AGC)5-6(AGA)0-1CACGACTGAAGCGACGC(AGC)12-33(ACC)0-1. In this study, only several alleles were sequenced, which means that it cannot rule out the possibility that there are different repeat structures in other alleles at the same locus and even in the same alleles of different individuals because sequence polymorphism may appear in the alleles with same length (Spolnicka et al, 2008).…”