Sequence analysis of four caprine mitochondria DNA lineages

Abstract

The complete mitochondrial DNA (mtDNA) (16640bp in length) was sequenced from four Chinese goat lineages representing the four major mtDNA haplogroups in goats. A total of 124 single nucleotide polymorphisms (SNPs) were found in encoding regions, and the overall ratio of transitions:transversions was 40:1 revealing a heavy transition/transversion rate in domestic goats. Eighteen non-synonymous sites were found for the total number of SNPs; the sites did not affect the predicted functions of protein for these four goat mtDNA lineages. In the region for coding tRNA and rRNA, SNPs occurred in loops, unstructured single strand and stems that were conformed with the principle of G-U pairing. We came to the conclusion that these substitutions could not change secondary structure of RNAs, and there was no positive selection on goat mitochondrial coding region according to the result of dN/dS (0.0399-0.1529) by comparing the goat with other reported mitochondrial genomes

Genetic diversity analysis among Indian goat breeds based on mitochondrial DNA

“…The overall ratio of transition vs transversion was 45.84 which revealed the transition bias in Indian goats. In Chinese goats, the transition: transversion was 40:1 which also revealed a heavy transition bias in the domestic goats (Wu et al 2009(Wu et al , 2012.…”