Sequence Analysis of Mitochondrial 16S Ribosomal RNA Gene Fragment in the Two Populations of Armigeres (Armigeres) subalbatus (Culcidae: Diptera)

Kohli, Rashmi; Chaudhry, S.

doi:10.1508/cytologia.72.83

Cited by 3 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, Chillar (2008) covered three different parameters of species discrimination by studying the polytene chromosome characterstics, morphometric analysis of head region (maxillary palps, palpomeres, proboscis, antenna), wing venation and sequence details of ITS2 and COII gene. According to him, Anopheles subpictus is represented by only two sibling species, A (inland) and B (coastal) as enough evidence could not be generated to support the earlier view for the presence of A, B, C and D. In relevance to these observartions, it is pertinent to add that the present results belong to species B whereas sufficient scope still exists to carry out sequence analysis of favourable regions of nuclear and mitochondrial DNA using specific primers and RFLP markers Kohli and Chaudhry, 2007;Kaura et al, 2009). …”

Section: Resultscontrasting

confidence: 53%

Inversion dynamics in some population of an emerging vector of malaria Anopheles (Celia) subpictus Grassi (Diptera: Culicidae)

Chaudhry

Kaura

Savita

et al. 2013

JANS

View full text Add to dashboard Cite

Abstract:The present paper deals with the polytene chromosome inversion polymorphism based genomic characterization of Anopheles subpictus Grassi (Culicidae: Diptera) which has attained the status of an emerging vector of malaria in Srilanka, West Bengal and some coastal parts of India. The inversion data of the present population from Hoshairpur, Punjab (pop.A), India has also been compared with five other populations of this species worked out earlier in this laboratory so as to have a comprehensive assessment of inversion dynamics in this taxon. From the percentage frequency of inversions it was also evident that both rural and urban populations of An. subpictus had nearly similar inversion frequencies. In addition to these observations, it was interesting to note that irrespective of the number of individuals sacrificed, cells studied and the number of aberrations encountered from each population, inversions always constituted 50% of the total mutational index. On the basis of the present comparative data of inversion polymorphism, it is logical to suggest that, similar to "meiotic drive" and "molecular drive" there is also a type of "inversion drive" which constantly changes population genomics to augment competitive fitness of the species. Our recent studies on the r DNA ITS2 sequence variations also suggest this contension.

show abstract

Section: Resultscontrasting

confidence: 53%

Inversion dynamics in some population of an emerging vector of malaria Anopheles (Celia) subpictus Grassi (Diptera: Culicidae)

Chaudhry

Kaura

Savita

et al. 2013

JANS

View full text Add to dashboard Cite

show abstract

DNA Fingerprinting and Phylogenetics of Five Species Of GenusCulexUsing ITS2 Sequence (Diptera: Culicidae)

2011

Self Cite

View full text Add to dashboard Cite

-The present paper deals with the ITS2 sequence based interspecifi c variations and molecular phylogenetics of fi ve Oriental species of genus Culex viz: Cx. quinquefasciatus, Cx. vishnui, Cx. bitaeniorhynchus, Cx. tritaeniorhynchus and Cx. mimeticus. The length of ITS2 sequence varied from 323 to 410 bp with G:C content ranging from 50.7% to 66.5%. The sequence carried indels at 221 positions while the transition to transversion (ts/tv) ratio ranged from 0.41 to 0.76 in which Cx. tritaeniorhynchus had the lowest ts/tv value of 0.04 while Cx. mimeticus had the highest value of 0.76. The most frequent transversions were of the A:T type. By using the sequence of Anopheles stephensi taken as an outgroup, the maximum parsimony based phylogenetic tree with threshold (considering only the transversions) showed that An. stephensi and Cx. quinquefasciatus were supported by 100% bootstrap value beyond which all other species got bifurcated into two clades in which one clade consisted of Cx. tritaeniorhynchus + Cx. mimeticus with a bootstrap value of 62.8% while the other consisted of Cx. bitaeniorhynchus + Cx. vishnui with a value of 19.5%.

show abstract

Barcoding of Italian mosquitoes (BITMO): generation and validation of DNA barcoding reference libraries for native and alien species of Culicidae

Bisaglia,

Castelli,

Soresinetti

et al. 2024

Parasites Vectors

View full text Add to dashboard Cite

Background Mosquitoes (Culicidae), as disease vectors, represent a risk for human health worldwide. Repeated introductions of alien mosquito species and the spread of invasive species have been recorded in different countries. Traditionally, identification of mosquitoes relies on morphological observation. However, morphology-based identification is associated with a number of potential disadvantages, such as the high level of specialisation of the operator and its limited applicability to damaged samples. In these cases, species identification is achieved through molecular methods based on DNA amplification. Molecular-based taxonomy has also enabled the development of techniques for the study of environmental DNA (eDNA). Previous studies indicated the 16S mitochondrial ribosomal RNA (rRNA) gene as a promising target for this application; however, 16S rRNA sequences are available for only a limited number of mosquito species. In addition, although primers for the 16S rRNA gene were designed years ago, they are based on limited numbers of mosquito sequences. Thus, the aims of this study were to: (i) design pan-mosquito 16S rRNA gene primers; (ii) using these primers, generate a 16S rRNA gene mosquito reference library (with a focus on mosquitoes present in Italy); and (iii) compare the discriminatory power of the 16S rRNA gene with two widely used molecular markers, cytochrome c oxidase subunit 1 mitochondrial gene (COI) and internal transcribed spacer 2 (ITS2). Methods A total of six mosquito genera (28 mosquito species) were included in this study: Aedes (n = 16 species), Anopheles (5 species), Coquillettidia (1 species), Culex (3 species), Culiseta (2 species) and Uranotaenia (1 species). DNA was extracted from the whole mosquito body, and more than one specimen for each species was included in the analysis. Sanger sequencing was used to generate DNA sequences that were then analysed through the Barcode of Life Data Systems (BOLD). Phylogenetic analyses were also performed. Results Novel 16S rDNA gene, COI and ITS2 sequences were generated. The 16S rRNA gene was shown to possess sufficient informativeness for the identification of mosquito species, with a discriminatory power equivalent to that of COI. Conclusions This study contributes to the generation of DNA barcode libraries, focussed on Italian mosquitoes, with a significant increase in the number of 16S rRNA gene sequences. We hope that these novel sequences will provide a resource for studies on the biodiversity, monitoring and metabarcoding of mosquitoes, including eDNA-based approaches. Graphical abstract

show abstract

Sequence Analysis of Mitochondrial 16S Ribosomal RNA Gene Fragment in the Two Populations of Armigeres (Armigeres) subalbatus (Culcidae: Diptera)

Cited by 3 publications

References 15 publications

Inversion dynamics in some population of an emerging vector of malaria Anopheles (Celia) subpictus Grassi (Diptera: Culicidae)

Inversion dynamics in some population of an emerging vector of malaria Anopheles (Celia) subpictus Grassi (Diptera: Culicidae)

DNA Fingerprinting and Phylogenetics of Five Species Of GenusCulexUsing ITS2 Sequence (Diptera: Culicidae)

Barcoding of Italian mosquitoes (BITMO): generation and validation of DNA barcoding reference libraries for native and alien species of Culicidae

Contact Info

Product

Resources

About