Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species

Will, G; Jauris-Heipke, Sigrid; Schwab, Ernie; Busch, U; Rössler, Dieter; Soutschek, Erwin; Wilske, B; Preac-Mursic,

doi:10.1007/bf00221390

Cited by 86 publications

(79 citation statements)

References 42 publications

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“…[24][25][26][27] Nucleotide sequence variation of a target gene may actually represent the genetic distance of phylogenetic divergence between or within the genospecies of Borrelia isolates. 27,31,34,35 In this study, the phylogenetic analysis of OspA gene demonstrates a high sequence homogeneity among Borrelia isolates within the genospecies of B. burgdorferi sensu stricto, and all of the Taiwan isolates exhibit almost identical nucleotide sequences of OspA gene (see Table 3). Further investigation of the sequence anlysis of other target genes, such as the genes encoding the OspC and 5S-23S intergenic spacer region, of these Taiwan isolates would help to elucidate the genetic divergence of Borrelia isolates in Taiwan.…”

Section: Discussionmentioning

confidence: 61%

An OspA-based genospecies identification of Lyme disease spirochetes (Borrelia burgdorferi) isolated in Taiwan.

Shih

Chao²

2002

The American Journal of Tropical Medicine and Hygiene

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Abstract. To further identify the genospecies of Lyme disease spirochetes (Borrelia burgdorferi) isolated in Taiwan, we analyzed the genomic identities of these Taiwan isolates (TWKM1-7) by genospecies-specific polymerase chain reaction (PCR) assay, restriction fragment length polymorphism (RFLP) analysis, and gene sequencing based on the OspA gene sequences of B. burgdorferi sensu lato. PCR analysis indicates that all of these Taiwan isolates were genetically related to the genospecies of B. burgdorferi sensu stricto by their differential reactivities with genospeciesspecific PCR primers. After cleavage by DraI, three different RFLP patterns in relation to three different genospecies of Lyme disease spirochetes were observed, and all of these Taiwan isolates were affiliated with the genospecies of B. burgdorferi sensu stricto. The phylogenetic analysis also reveals that the sequence similarity of PCR-amplified OspA gene of these Taiwan isolates is highly homogeneous, with a homogeneity of more than 99.8% within the genospecies of B. burgdorferi sensu stricto. These results confirm that the genomic identities of these Taiwan isolates belong to the genospecies of B. burgdorferi sensu stricto.

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Section: Discussionmentioning

confidence: 61%

An OspA-based genospecies identification of Lyme disease spirochetes (Borrelia burgdorferi) isolated in Taiwan.

Shih

Chao²

2002

The American Journal of Tropical Medicine and Hygiene

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“…Este sería el caso de la genoespecie identificada en Yucatán, B. garinii, conocida por ser la más heterogénea de todas las especies del complejo B. burgdorferi s.l. (46,47).…”

Section: Discussionunclassified

Prevalencia de Borrelia burgdorferi sensu lato en roedores sinantrópicos de dos comunidades rurales de Yucatán, México

et al. 2016

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Introducción. La enfermedad de Lyme es una zoonosis multisistémica causada por Borrelia burgdorferi sensu lato. Esta espiroqueta circula en un ciclo enzoótico entre un reservorio vertebrado primario y las garrapatas. Diferentes especies de roedores están identificadas por ser eficientes reservorios naturales para B. burgdorferi s.l.Objetivo. Estimar la prevalencia de B. burgdorferi s.l. en roedores sinantrópicos empleando dos comunidades rurales de Yucatán, México.Materiales y métodos. Se capturaron 123 roedores (94 Mus musculus y 29 Rattus rattus) para obtener muestras de tejidos de oreja y vejiga. Para detectar la presencia de B. burgdorferi s.l. en la muestras, se amplificaron los genes de la flagelina B (fla B) y las lipoproteínas de membrana externa, ospC y p66, empleado la reacción en cadena de la polimerasa. Los amplicones obtenidos fueron secuenciados.Resultados. La frecuencia de infección de B. burgdorferi s.l. en roedores fue de 36,5% para flaB (45/123), 10,5% (13/123) para p66 y 3,2% (4/123) para ospC. R. rattus tuvo una frecuencia de infección de 17,2% y M. musculus de 42,5%. La frecuencia de infección de B. burgdorferi s.l. en los tejidos estudiados fue de 11,3% (14/123) en vejigas y 17,0% (21/123) en orejas. No se encontraron diferencias estadísticas (p > 0,05) en la frecuencia de infección entre las dos muestras de tejido utilizadas para el diagnóstico. El gen ospC presentó un 98% de homología con la especie Borrelia garinii, una de las especies heterogéneas del complejo B. burgdorferi s.l. Conclusiones. Se concluye que los roedores presentan alta prevalencia de infección con B. burgdorferi s.l., y ambas especies, M. musculus y R. rattus, podrían estar jugando un papel importante en el mantenimiento de esta bacteria en comunidades rurales de Yucatán, México.<pre style="line-height: 200%; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;">Introducción. La enfermedad de Lyme es una zoonosis multisistémica causada por Borrelia burgdorferi sensu lato. Esta espiroqueta circula en un ciclo enzoótico entre un reservorio vertebrado primario y las garrapatas. Diferentes especies de roedores están identificadas por ser eficientes reservorios naturales para B. burgdorferi s.l.</pre><pre style="line-height: 200%; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;">Objetivo. Estimar la prevalencia de B. burgdorferi s.l. en roedores sinantrópicos empleando dos comunidades rurales de Yucatán, México.</pre><pre style="line-height: 200%; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;">Materiales y métodos. Se capturaron 123 roedores (94 Mus musculus y 29 Rattus rattus) para obtener muestras de tejidos de oreja y vejiga. Para detectar la presencia de B. burgdorferi s.l. en la muestras, se amplificaron los genes de la flagelina B (fla B) y las lipoproteínas de membrana externa, ospC y p66, empleado la reacción en cadena de la polimerasa. Los amplicones obtenidos fueron secuenciados.</pre><pre style="line-height: 200%; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;">Resultados. La frecuencia de infección de B. burgdorferi s.l. en roedores fue de 36,5% para flaB (45/123), 10,5% (13/123) para p66 y 3,2% (4/123) para ospC. R. rattus tuvo una frecuencia de infección de 17,2% y M. musculus de 42,5%. La frecuencia de infección de B. burgdorferi s.l. en los tejidos estudiados fue de 11,3% (14/123) en vejigas y 17,0% (21/123) en orejas. No se encontraron diferencias estadísticas (p > 0,05) en la frecuencia de infección entre las dos muestras de tejido utilizadas para el diagnóstico. El gen ospC presentó un 98% de homología con la especie Borrelia garinii, una de las especies heterogéneas del complejo B. burgdorferi s.l.</pre><pre style="line-height: 200%; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;">Conclusiones. Se concluye que los roedores presentan alta prevalencia de infección con B. burgdorferi s.l., y ambas especies, M. musculus y R. rattus, podrían estar jugando un papel importante en el mantenimiento de esta bacteria en comunidades rurales de Yucatán, México.</pre>

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“…The hete rogeneity of the causative strains is a challenge for the microbiological diagnosis of Lyme borreliosis in Europe. The heterogeneity observed among ospA sequences of European strains (especially regarding B. garinii), has been used for the development of diagnostic tools (Wilske et al, 1993;Will et al, 1995;Michel et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…To investigate the correspondence of strains to OspA serotype types the obtained sequences were compared with the strains of serotypes described for B. garinii (Wilske et al, 1993;Will et al, 1995;Wilske et al, 2007) from GenBank. Among the 13 ospA sequences obtained from ticks in this study, 5 different genotypes were identified.…”

Section: Sequence Analysismentioning

confidence: 99%

The prevalence of Borrelia burgdorferi sensu lato in questing Ixodes ricinus ticks in Norway

Rosef¹,

Radzijevskaja

Kløcker³

et al. 2014

Biologija

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Norway represents the northern limit in the geographical distribution of Ixodes ricinus ticks in Europe. During the last decade an expansion of the range of this tick further north and at higher altitudes has been detected. This could affect the spread of Borre lia burgdorferi sensu lato pathogens in new territories and increase the risk of human infection in the country. The aim of the present study was to determine the prevalence of B. burgdorferi s.l. in I. ricinus ticks in different localities of Norway. Ticks were collected in 24 locations from northwest to southeast Norway during springsummer 2004 and 2006-2008. We used fla gene as a target for amplification of spirochete DNA. Multiplex PCR based on ospA gene of B. burgdorferi s.l. was used for species identification. The heterogeneity of B. garinii strains was investigated using ospAbased sequencing analysis. B. burgdorferi s.l. was detected in 171 (7.2%) out of 2360 I. ricinus ticks analyzed. The infection rate varied from zero to 32% in the investigated locations. Infection rate in adults (11.9%) was significantly higher than in nymphs (4.7%). The overall infection rate in ticks from western Norway (4.0%) was significantly lower than in those from southern Norway (10%). Four B. burgdorferi s.l. genospecies were identified, and the most common was B. afzelii (71.9.0%), followed by B. garinii (12.9%), B. burgdorferi s.s. (7%) and B. valaisiana (1.2%). A total of eight (4.8%) of the infected ticks harboured mixed infections. Phylogenetic relationship between B. garinii strains and their cor respondence to OspA serotype types was compared with the sequences registered in GenBank database. Five genotypes of B. garinii derived from ticks were identified. The most frequent was OspA type 6.

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Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species

Cited by 86 publications

References 42 publications

An OspA-based genospecies identification of Lyme disease spirochetes (Borrelia burgdorferi) isolated in Taiwan.

An OspA-based genospecies identification of Lyme disease spirochetes (Borrelia burgdorferi) isolated in Taiwan.

Prevalencia de Borrelia burgdorferi sensu lato en roedores sinantrópicos de dos comunidades rurales de Yucatán, México

The prevalence of Borrelia burgdorferi sensu lato in questing Ixodes ricinus ticks in Norway

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