Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus.

Gomi, Sumiko; Majima, Kei; Maeda, Susumu

doi:10.1099/0022-1317-80-5-1323

Cited by 396 publications

(271 citation statements)

References 74 publications

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“…This is similar to the 24/30 matches for the BmNPV (Gomi et al, 1999) and OpMNPV (Ahrens et al, 1997) palindrome sequence, and the 26/30 matches for the AcMNPV (Ayres et al, 1994) palindrome consensus (Fig. 3a).…”

Section: Sequence Analysis Of Mavinpv Genomesupporting

confidence: 79%

“…The apparent size of the MaviNPV genome (111.95 kbp in this study) is smaller than that of AcMNPV (133.9 kbp) (Ayres et al, 1994) and BmNPV (128.4 kbp) (Gomi et al, 1999). Based on the sequences of 29 baculoviral core genes in phylogenetic analyses in this study, MaviNPV belongs to group I NPVs and is closely related to AcMNPV and BmNPV.…”

Section: Introductionmentioning

confidence: 62%

“…MaviNPV shares 125 ORFs with AcMNPV, 123 with BmNPV, 90 with LdMNPV and 62 with CpGV, and these homologous ORFs had mean amino acid identities of 79.2, 78.6, 34.8 and 27.1 %, respectively. In the 132 genes shared between BmNPV and AcMNPV, the average amino acid identity is 93 % (Gomi et al, 1999). There are fewer shared genes between MaviNPV and BmNPV (123), and between MaviNPV and AcMNPV (125); the mean amino acid identities of these shared genes are also lower (79.2 and 78.6 %, respectively).…”

Section: Resultsmentioning

confidence: 99%

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Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus

Chen

Lee

et al. 2008

Journal of General Virology

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The complete genome of the Maruca vitrata nucleopolyhedrovirus (MaviNPV) isolated from the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), was sequenced. It was found to be 111 953 bp in length, with an overall 39 % G+C content, and contained 126 open reading frames (ORFs) encoding predicted proteins of over 50 aa. The gene content and gene order of MaviNPV have the highest similarity to those of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and their shared homologous genes are 100 % collinear. In fact, MaviNPV seems to be a mini-AcMNPV that is native to Taiwan and possesses a smaller genome with fewer auxiliary genes than the AcMNPV type species. Except for one ORF (Mv74), all of the MaviNPV ORFs have homologues in the AcMNPV genome. MaviNPV is the first lepidopteranspecific baculovirus to lack homologues of vfgf and odv-e66. In addition, MaviNPV lacks the baculovirus repeat ORF (bro) gene that corresponds to AcMNPV ORF2. Five homologous regions (hrs) were located within the MaviNPV genome, and these contained a total of 44 imperfect palindromes. Phylogenetic analysis of the whole genome revealed that MaviNPV was separated from the common ancestor of AcMNPV and Bombyx mori nucleopolyhedrovirus before these two viral species diverged from each other. Moreover, replication of MaviNPV in several cell lines and an egfp-MaviNPV infection assay revealed that IPLB-LD-652Y cells are only partially permissive to MaviNPV, which supports our conclusion that MaviNPV is a distinct species of the group I lepidopteran NPVs.

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Section: Sequence Analysis Of Mavinpv Genomesupporting

confidence: 79%

Section: Introductionmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 99%

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Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus

Chen

Lee

et al. 2008

Journal of General Virology

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“…To avoid the influence of viral infection, we designed a cross way to study the function of Bm-svv, using incubation of baculovirus with non-permissive cell lines. Although BmNPV and AcNPV have approximately 93 % similarity to the amino acid sequence with each other (Gomi et al 1999), AcNPV infects Sf 9 and Tn 5 cells, but not BmN cells and it induces Spli cell apoptosis (Hink 1970;Chejanovsky and Gershburg 1995). However, BmNPV only can infect BmN cells, but not Sf 9, Tn 5, and Spli cells.…”

Section: Discussionmentioning

confidence: 98%

“…Recent nucleotide sequence analyses of B. mori NPV (BmNPV) have found that its ORFs show approximately 93 % similarity to the amino acid sequence of AcNPV (Gomi et al 1999). Although these genes exhibit a close relationship, they present significant differences in host range.…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of a survivin-like gene in Bombyx mori

Tang

Wu³

et al. 2013

Cytotechnology

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The survivin (svv) gene is a newly discovered member of the inhibitors of apoptosis gene family. In recent years, svv has been confirmed to have an anti-apoptosis function and to play a critical role in cell division. We identified a survivin-like gene in the silkworm, Bombyx mori (Bm-svv). In this study, to gain insight into its function, a baculovirus expression system was used to express the Bm-svv gene in insect cell lines. The recombinant viruses were then used as a vector to transform insect cells, and cell activity was determined using the Cell Counting Kit-8 (CCK-8), which is usually employed for detecting mammalian cell number. The results indicated that the Bm-svv gene plays a role in the cell growth arrest or apoptosis induced by viruses. Furthermore, the CCK-8 kit is effective in determining the activity of insect cells.

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Insect-virus relationships: Sifting by informatics

2001

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Several groups of large DNA viruses successfully utilise the rich resource provided by insect hosts. Defining the mechanisms that enable these pathogens to optimise their relationships with their hosts is of considerable scientific and practical importance, but our understanding of the processes involved is, as yet, rudimentary. Here we describe an informatics-based approach that uses comparison of viral genomic sequences to identify candidate genes likely to be specifically involved in this process. We hypothesise that such genes should satisfy two essential criteria, namely, that they should be (i) present in those members of a virus family that infect insects, but absent from those that infect other hosts, and (ii) found in at least two unrelated taxa of insect viruses. These criteria currently identify six groups of viral genes, including one that encodes the fusolin/gp37 proteins. Demonstration that the fusolin/gp37 proteins can enhance oral infectivity of insect viruses provides a primary validation of this approach to the examination of insect-virus relationships.

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Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus.

Cited by 396 publications

References 74 publications

Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus

Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus

Functional analysis of a survivin-like gene in Bombyx mori

Insect-virus relationships: Sifting by informatics

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