Sequence analysis of the msp4 gene of Anaplasma ovis strains

Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Detection of Anaplasma ovis

“…The primer set was designed on the basis of the MSP4 gene of A. ovis (Table 1). The MSP4 genes of A. ovis from the Yongjing, Yuzhong, Zhangjiachuan, and Chengde isolates were amplified by PCR, as described previously (2). PCR products were cloned into a pGEM-T Easy vector (Promega, Beijing, China) and subjected to sequencing (Sangon Biotech, Shanghai, China).…”

“…DNA extraction and preparation were performed using a DNeasy blood and tissue kit (Qiagen, China), following the manufacturer's instructions. These DNA samples were analyzed using both LAMP and a PCR method (2). The PCR primers were forward primer MSP4-5 (5Ј-GGGAGCTCCTATGAATTACAGA GAATTGTTTAC-3Ј) and reverse primer MSP4-3 (5Ј-CCGGATCCTTAGCTG AACAGGAATCTTGC-3Ј).…”

“…PCR using the 16S rRNA gene (15) and major surface protein 4 (MSP4) gene (2) and the reverse line blotting method (1), have been shown to be of great diagnostic value in the identification of A. ovis infections. However, these methods are timeconsuming and require expensive experimental devices and a high degree of laboratory experience in molecular biology.…”

The Biological Survey of Canada: A Personal History

“…Ticks of the genus Dermacentor are the vectors of A. ovis in the western United States of America (de la Fuente et al 2006Fuente et al , 2007.…”

Tolerance to Tick-Borne Diseases in Sheep: Highlights of a Twenty-Year Experience in a Mediterranean Environment

“…In particular the presence of A. ovis was confirmed by specific polymerase chain reactions (PCRs) for Anaplasma spp. (Stuen et al, 2003) and A. ovis (de la Fuente et al, 2005;de la Fuente et al, 2007). Then PCRs followed by reverse line blot hybridization of the amplified 18SrRNA gene from Theileria and Babesia species, was used to detect specific probes for Theileria/Babesia catch all, Theileria sp1 china, Theileria sp2 chinal, T.…”