Sequence analysis of the mtDNA region correlated with Shaan 2A cytoplasmic male sterility in rapeseed (Brassica napus L.)

Havlíčková, Lenka; Čurn, Vladislav; Jozová, Eva; Kučera, V.; Vyvadilová, M.; Klíma, Miroslav

doi:10.17221/144/2012-cjgpb

Cited by 2 publications

(3 citation statements)

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“…In this study, the S and N molecular markers developed are derived from the indel differences in mitochondrial DNA between fertile cytoplasmic N and sterile cytoplasmic S, while the R and r molecular markers developed are derived from core SNP differences between the restorer gene R and the sterility gene r in the nucleus [ 12 , 31 ]. The combination of these molecular markers can rapidly and accurately identify the CMS lines, maintainer lines, restorer lines and hybrids of the pol and shaan CMS systems [ 2 , 31 ]. This can be applied to the identification of both the maintainer or restorer relationship in inbred lines, and the rapeseed purity of the cytoplasmic three-line rapeseed hybrids.…”

Section: Discussionmentioning

confidence: 99%

“…The rapeseed CMS lines shaan 2A [ 2 , 3 ] and pol A [ 1 ] of rapeseed and their maintainers shaan 2B and pol B [ 3 ] were sourced from Dianrong Li. The rapeseed CMS hybrid Qinyou 7 of rapeseed and its sterile line shaan 3A, its maintainer shaan 3B and its restorer K407 were sourced from Dianrong Li.…”

Section: Methodsmentioning

confidence: 99%

“…The discovery of CMS was a milestone advancement in the development of global rapeseed cultivation, introducing a new era of heterosis utilization in Brassica napus ( B. napus ). More than 10 CMS types have been discovered in B. napus , among them pol [ 1 ], shaan [ 2 , 3 ], ogu [ 4 , 5 ], nap [ 6 ], tour [ 7 ], nca and hau [ 8 ]. The pol and shaan types belong to the same type of CMS system, sharing common fertility restorers [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

Zhang

Song

et al. 2023

Plants

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Cytoplasmic male sterility (CMS) is the main mechanism employed to utilize the heterosis of Brassica napus. CMS three-line rapeseed hybrids have dramatically enhanced yield and brought about the global revolution of hybrid varieties, replacing conventional crop varieties. Over the last half century, China has led the development of hybrid Brassica napus varieties. Two sterile lines, polima (pol) and shaan 2A, were of particular importance for the establishment of three-line hybrid systems in rapeseed, which has opened up a new era of heterosis utilization. However, in current breeding practices, it takes up to three years to identify the restorer or maintainer relationship and the cytoplasmic type of any inbred material. This greatly affects the breeding speed of new varieties and inhibits the rapid development of the rapeseed industry. To address this problem, we developed a set of molecular markers for the identification of fertile cytoplasmic gene N and sterile cytoplasmic gene S, as well as for the fertile nucleus gene R and sterile nucleus gene r, based on differences in the gene sequences between the CMS line, maintainer line and restorer line of Brassica napus. Combining these markers can accurately identify the CMS line, maintainer and restorer of both the pol and shaan systems, as well as their hybrids. These markers can not only be used to identify of the maintainer and restorer relationship of inbred materials; they can also be used as general molecular markers to identify the CMS-type hybrid purity of pol and shaan systems.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

Zhang

Song

et al. 2023

Plants

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CMS systems in rapeseed and their use in the breeding of domestic hybrids

Anisimova

Dubovskaya

2020

Trudy po prikladnoy botanike, genetike i selektsii

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Development of heterotic hybrids is the most efficient approach to solve the problem of increasing the yield of rapeseed (Brassica napus L.), a leading oilseed crop. The cytoplasmic male sterility (CMS), widely used in rapeseed hybrid seed production, makes it possible to control hybridization between female and male lines. A review of publications on the nature of CMS systems in rapeseed and their utilization in breeding is presented. In rapeseed there are more than 10 known CMS systems of alloplasmic and homoplasmic origin. The male sterility character proved to be determined by chimeric mitochondrial genes, characterized by the presence of novel transcribed open reading frames (orf). Mitochondrial CMS genes associated with nap, pol, ogu and Nsa sterility types as well as nuclear Rf genes for pollen fertility restoration were identified. Molecular marker systems for identification of CMS-inducing and male fertility restoring genes were developed. The ogu, pol, MSL and inap CMS systems are commonly used for producing industrial rapeseed hybrids. The State Register of the Russian Federation for 2019 contains rapeseed hybrids of only foreign origin. Main achievements in domestic rapeseed hybrid production are highlighted. Research and breeding institutions developed new source material for rapeseed hete rotic hybrid breeding in various regions of the country. The sterility and fertility restoration sources were received from Canadian and French institutions as well as from domestic working collections. The yield structure traits did not deteriorate after transferring hybrid maternal lines to the sterile cytoplasm, while the glucosinolate content increased when pollen fertility restoring genes were transferred into paternal lines. Dihaploid (androclinium) lines and in vitro culture of unfertilized ovules were used to accelerate the breeding process. Experimental hybrids were developed using pol and ogu CMS.

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Sequence analysis of the mtDNA region correlated with Shaan 2A cytoplasmic male sterility in rapeseed (Brassica napus L.)

Cited by 2 publications

References 6 publications

A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

A Set of Molecular Markers to Accelerate Breeding and Determine Seed Purity of CMS Three-Line Hybrids in Brassica napus

CMS systems in rapeseed and their use in the breeding of domestic hybrids

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