Sequence analysis of the turkey coronavirus nucleocapsid protein gene and 3′ untranslated region identifies the virus as a close relative of infectious bronchitis virus

Breslin, Jamie J.; Smith, Lynda G.; Fuller, Frederick J.; Guy, James S.

doi:10.1016/s0168-1702(99)00117-3

Cited by 58 publications

(52 citation statements)

References 23 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Both pf/CH/LKQ3/03 and tl/CH/LDT3/ 03 N protein ORFs were 1230 nt in length, within the typical range for most IBV strains and turkey coronaviruses (Boursnell et al, 1987;Breslin et al, 1999b).…”

Section: N Genementioning

confidence: 76%

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Liu

Chen

et al. 2005

Journal of General Virology

132

View full text Add to dashboard Cite

Coronavirus-like viruses, designated peafowl/China/LKQ3/2003 (pf/CH/LKQ3/03) and teal/China/LDT3/2003 (tl/CH/LDT3/03), were isolated from a peafowl and a teal during virological surveillance in Guangdong province, China. Partial genomic sequence analysis showed that these isolates had the S-3-M-5-N gene order that is typical of avian coronaviruses. The spike, membrane and nucleocapsid protein genes of pf/CH/LKQ3/03 had >99 % identity to those of the avian infectious bronchitis coronavirus H120 vaccine strain (Massachusetts serotype) and other Massachusetts serotype isolates. Furthermore, when pf/CH/LKQ3/03 was inoculated experimentally into chickens (specific-pathogen-free), no disease signs were apparent. tl/CH/LDT3/03 had a spike protein gene with 95 % identity to that of a Chinese infectious bronchitis virus (IBV) isolate, although more extensive sequencing revealed the possibility that this strain may have undergone recombination. When inoculated into chickens, tl/CH/LDT3/03 resulted in the death of birds from nephritis. Taken together, this information suggests that pf/CH/LKQ3/03 might be a revertant, attenuated vaccine IBV strain, whereas tl/CH/LDT3/03 is a nephropathogenic field IBV strain, generated through recombination. The replication and non-pathogenic nature of IBV in domestic peafowl and teal under field conditions raises questions as to the role of these hosts as carriers of IBV and the potential that they may have to transmit virus to susceptible chicken populations. INTRODUCTIONCoronaviruses (family Coronaviridae) belong to the order Nidovirales and contain a positive-stranded RNA genome that ranges from 27 to 31 kb in size (Cavanagh, 1997). Members of the family Coronaviridae infect a wide range of hosts and have been classified into three groups on the basis of antigenicity, genome organization and sequence similarity. Usually, coronaviruses infect only their normal target host species. It has, however, been reported that some strains of canine coronavirus and human coronavirus 229E can infect other, non-target species without causing disease (Barlough et al., 1984(Barlough et al., , 1985. The recent emergence of severe acute respiratory syndrome coronavirus (SARSCoV), which has been classified tentatively into group 2, has focused a great deal of interest on this virus family (Holmes, 2003). It was reported that SARS-CoV-like viruses were isolated from Himalayan palm civets (Guan et al., 2003) and ferrets (Mustela furo). Moreover, domestic cats (Felis domesticus) are susceptible to infection by SARSCoV, suggesting that the reservoir for this pathogen might involve a range of animal species (Martina et al., 2003).Infectious bronchitis virus (IBV), together with genetically related coronaviruses of turkey and pheasant, belongs to the group 3 coronaviruses. IBV is a pleomorphic, enveloped virus with club-shaped surface projections (spikes) and a single-stranded, positive-sense RNA genome of >27 kb in length (Boursnell et al., 1987). Upon virus entry into cells, a 39-coterminal nested set of six ...

show abstract

Section: N Genementioning

confidence: 76%

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Liu

Chen

et al. 2005

Journal of General Virology

132

View full text Add to dashboard Cite

show abstract

“…In addition, these analyses demonstrate that the avian coronaviruses, TCoV and IBV, constitute a distinct genotype within the Coronavirus genus. A phylogenetic tree is shown in Figure 1 that compares the complete N protein amino acid sequences of three TCoV strains (Breslin et al, 1999b) with published sequence data for selected IBV strains, and representative group 1 and group 2 mammalian coronaviruses, TGEV and BCV, respectively. This phylogenetic tree demonstrates that TCoV is very closely related to IBV, to such an extent that they cannot be distinguishe d based on this region of the genome.…”

Section: Antigenic/genomic Characterization Of Tcovmentioning

confidence: 99%

Turkey coronavirus is more closely related to avian infectious bronchitis virus than to mammalian coronaviruses: A review

Guy

2000

Avian Pathology

View full text Add to dashboard Cite

Turkey coronavirus (TCoV) is the cause of an acute highly contagious enteric disease of turkeys. In recent years, TCoV has been increasingly recognized in North America as an important pathogen of young turkeys, resulting in economic loss due to impaired growth and poor feed conversion. While the epidemiology and pathogenesis of TCoV have been extensively studied, TCoV remains one of the least characterized of the known coronaviruses.Avian and mammalian coronaviruses have been subdivided into distinct antigenic/genotypic groups; however, classification of TCoV has been controversial. Previous studies indicated that TCoV was closely related to bovine coronavirus and other group 2 mammalian coronaviruses, but more recent antigenic and genome sequence analyses contradict these findings and, instead, provide evidence that TCoV is closely related to avian infectious bronchitis virus (IBV). Additionally, experimental studies have indicated that the host range of TCoV, once thought to be restricted to turkeys, includes chickens. These studies have raised additional questions regarding the classification of TCoV; particularly, whether IBV and TCoV are taxonomically distinct viruses, or whether TCoV is merely a variant of IBV.Sequence analyses of TCoV have given credence to the idea that TCoV is a variant of IBV, as these studies have shown that TCoV and IBV are very closely related. However, these studies have been limited to only three TCoV strains and relatively small portions of the TCoV genome. TCoV is readily distinguished from IBV based on antigenic and biological differences, and these differences suggest that TCoV should be considered a distinct virus species.Additional studies will be needed to better define the relationship between TCoV and IBV, and to resolve this taxonomic question. Based on our current understanding, it seems prudent to consider TCoV and IBV as distinct virus species that share a close phylogenetic relationship and together comprise group 3 of the coronavirus major antigenic groups.

show abstract

“…They are pleomorphic positive-sense single-stranded RNA viruses measuring 60-220 nm in diameter. 3,11 The characteristic feature of the coronaviral morphology is the presence of long (12-24 nm), widely spaced petal-or pear-shaped surface projections, which impart to the virus the appearance of a solar corona. 14 Three structural proteins have been identified in the virus.…”

mentioning

confidence: 99%

“…Infectious bronchitis virus (IBV) and turkey coronavirus (TCoV) belong to group III of Coronaviridae. 3,7,15 Turkey coronavirus causes an acute, highly infec- tious disease affecting turkeys of all ages. The disease is known by various names.…”

mentioning

confidence: 99%

“…Recent studies have strongly indicated a close antigenic as well as genetic relationship between these 2 important viruses. 1,3,7,15 The complete nucleotide sequence of the N gene and a portion of the M gene were analyzed, and their projected amino acid sequences were found to be very similar (more than 90%) to those of IBV M and N proteins. 1,3,15 The amplification of the IBV genome can be explained on the basis of the abovementioned observation.…”

mentioning

confidence: 99%

See 1 more Smart Citation

A Reverse Transcriptase—Polymerase Chain Reaction Assay for the Diagnosis of Turkey Coronavirus Infection

et al. 2003

View full text Add to dashboard Cite

Abstract. This study reports on the development of a reverse transcriptase-polymerase chain reaction (RT-PCR) for the specific detection of turkey coronavirus (TCoV). Of the several sets of primers tested, 1 set of primers derived from the P gene and 2 sets derived from the N gene of TCoV could amplify the TCoV genome in the infected samples. The RT-PCR was sensitive and specific for TCoV and did not amplify other avian RNA and DNA viruses tested except the infectious bronchitis virus (IBV). To overcome the problem of IBV amplification, a set of separate primers was designed from the spike protein gene of IBV. The RT-PCR under the same conditions as above could effectively differentiate between TCoV and IBV. The closely related bovine coronavirus and transmissible gastroenteritis virus of pigs were differentiated from TCoV using the same RT-PCR with slight modifications. The results of RT-PCR correlated well with the results of the immunofluorescent test for the same samples tested at the Purdue University Animal Disease Laboratory, West Lafayette, Indiana. The nucleotide sequence and projected amino acid sequence comparison of the P gene of different isolates of TCoV from 5 different states in the United States revealed a close association among the different isolates of TCoV.Coronavirus belongs to the family Coronaviridae, a large group of ribonucleic acid (RNA) viruses that infect a wide range of avian and mammalian species. They are pleomorphic positive-sense single-stranded RNA viruses measuring 60-220 nm in diameter. 3,11 The characteristic feature of the coronaviral morphology is the presence of long (12-24 nm), widely spaced petal-or pear-shaped surface projections, which impart to the virus the appearance of a solar corona. 14

show abstract

Sequence analysis of the turkey coronavirus nucleocapsid protein gene and 3′ untranslated region identifies the virus as a close relative of infectious bronchitis virus

Cited by 58 publications

References 23 publications

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Turkey coronavirus is more closely related to avian infectious bronchitis virus than to mammalian coronaviruses: A review

A Reverse Transcriptase—Polymerase Chain Reaction Assay for the Diagnosis of Turkey Coronavirus Infection

Contact Info

Product

Resources

About