Sequence analysis of the VP2 gene hypervariable region of infectious bursal disease viruses from India

Kataria, R. S.; Tiwari, Ashok Kumar; Butchaiah, G.; Kataria, J.M.; Skinner, Michael A.

doi:10.1080/03079450120078699

Cited by 19 publications

(14 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years amino acid change D212N found in the same hydrophilic region 'A', may also be essential in structural stability of the hypervariable region, VP2 protein [30]. The trend of mutation is followed naturally in the existing genetic pool of Indo-Pak region [8]. Some of US variant and French vvIBDVs matched the similar ongoing change [9,41].…”

Section: Discussionmentioning

confidence: 96%

“…At nucleotide level, none of Pakistani samples showed 100% similarity. Within each group the local strains reflected divergence less than 2% but intragroup divergence reached up to 8.5% which may be due to wide temporal and geographical differences of these isolates [8]. The Japanese strain D49706.1 differ 4.2% from native vvIBDVs.…”

Section: Discussionmentioning

confidence: 99%

“…Under high selection pressure in immune populations there is emergence of new viral strains in targeted poultry flock. VP2, structural protein has a major virus neutralizing conformational epitopes in hypervariable region [8][9][10]. Antigenicity and virulence of the IBDV has been studied extensively on the genetic basis to control IBD [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Characterization of Infectious Bursal Disease Virus From Commercial Poultry in Pakistan

Khan¹,

Habib²,

Shah³

et al. 2017

Matrix sci. medica

View full text Add to dashboard Cite

Infectious bursal disease (IBD) is an immunosuppressive disease of young, growing chickens which results in impaired growth or mortality of rearing flocks. In the current era there is a re-emergence of very virulent Infectious Bursal Disease Viruses (vvIBDV) and classical variant (cv) IBDV strains which increased the financial losses of poultry industry worldwide. Recent studies were conducted to characterize the existing vvIBDVs prevailing in Pakistan. The suspected samples were collected from the field outbreaks during the period from 2014-2017. IBDV was detected by RT-PCR. The sequences of VP2 gene (hyper variable region) were determined and available details were aligned with sequences submitted inGenBank. Phylogenetic analysis reveals that both vvIBDV and classical variant strains were circulating in different regions of Pakistan. In Indo-Pak isolates, the presence of virulent markers, amino acids (A222, I242, Q253, I256 and S299) and "Serine rich-heptapeptide" indicated the presence of very virulent viruses. The presence of T284A isan indicator of vvIBDVs in local poultry farms. More than 99% similarity of Pakistani isolates with Indian sequences reflects the trans-boundary spread of IBD. In recent studies amino acid, Glutamine (Q) is present at position 221 (as reported in previous studies) rather than Histidine (H) in Pakistani sequences. It is investigated that Glutamic acid (E) is located at position 300 in minor hydrophilic region III of VP2 protein in all reported Pakistani isolates. It is the unique feature of indigenous strains. This study will be useful in understanding the origin and pathotypes of IBDV circulating in Pakistan.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterization of Infectious Bursal Disease Virus From Commercial Poultry in Pakistan

Khan¹,

Habib²,

Shah³

et al. 2017

Matrix sci. medica

View full text Add to dashboard Cite

show abstract

“…The importance of these findings has direct implications on the strategies involved in vaccine preparation and selection (Fussell, 1998). Changes in the pathogenicit y, antigenicity, or immunogenicity of IBDV derived from different host systems have been partially explained by alterations of the viral genome and epitopes (Ismail et al, 1988;Bayliss et al, 1990;Kibenge et al, 1991;Lana et al, 1992;Jackwood & Jackwood, 1994Vakharia et al, 1994;Jackwood & Sommer, 1997Ture et al, 1998;Eterradossi, et al, 1998Eterradossi, et al, , 1999Mundt, 1999;Boot et al, 2000;Kwon et al, 2000;Yamaguchi et al, 2000;Yu et al, 2000;Abdel-Alim & Saif 2001a,b;Brandt et al, 2001;Cavanagh, 2001;Hoque et al, 2001;Jackwood et al, 2001a,b;Kataria et al, 2001;Nagarajan et al, 2001;Toroghi et al, 2001;Van Loon et al, 2002). However, further work is required to gain a better understanding in correlating genomic and protein modifications with changes in the biological properties of IBDV.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the antigenic, immunogenic, and pathogenic variation of infectious bursal disease virus due to propagation in different host systems (bursa, embryo, and cell culture). I. Antigenicity and immunogenicity

2002

View full text Add to dashboard Cite

In vitro and in ovo virus neutralization assays were conducted to assess the role of different host systems in infectious bursal disease virus (IBDV) antigenic and immunogenic variation. Four different strains, two variant (1084 E and GLS) and two standard (Edgar and STC), were propagated separately in the bursa of Fabricius and embryos, and were compared with cell culture-adapted preparations of the homologous strains. Chicken polyclonal antisera were prepared against each IBDV and neutralizing antibody titres were determined. Normalized IBDV antibody concentrations were used in neutralization assays against homologous and heterologous IBDVs in 10-day-old specific pathogen free embryos. Both antigenic and immunogenic changes occurred in IBDVs evaluated, as evidenced by differences in the ability of normalized antibody to neutralize IBDV propagated in different host systems. Antibody induced by bursal-derived IBDV neutralized all isolates equally well, whereas antibody induced by cell culture-derived virus neutralized bursal-derived IBDV much less effectively.

show abstract

“…Th e BspMI cleavage site found in vvIBDV strains correlated with the amino acid position 222 (proline to alanine) in the major hydrophilic peak A of the VP2 hypervariable region. Th is amino acid exchange was conserved in all the vvIBDVs (Brown et al, 1994;Kataria et al, 2001).…”

Section: Figmentioning

confidence: 99%

Genotypic characterization of Indian isolates of infectious bursal disease virus strains by reverse transcription-polymerase chain reaction combined with restriction fragment length polymorphism analysis

Priyadharsini¹,

Senthilkumar²,

Raja³

et al. 2016

View full text Add to dashboard Cite

Summary. -Th e reverse transcription PCR (RT-PCR) combined with restriction fragment length polymorphism (RFLP) is used for the diff erentiation of classical virulent (cv), virulent (v) and very virulent (vv) strains of infectious bursal disease virus (IBDV) isolates from chicken bursal tissues in southern states of India. In the present study, six diff erent isolates (MB11, HY12, PY12, BGE14, VCN14 and NKL14) of IBDV strains were subjected for genotyping along with vaccine virus (Georgia, intermediate strain) using RT-PCR for amplifi cation of a 743 bp sequence in the hypervariable region of VP2 gene followed by restriction enzyme digestion with 5 diff erent restriction enzymes (BspMI, SacI, HhaI, StuI and SspI). Th e RT-PCR products obtained from vvIBDV strains were digested by SspI enzyme except PY12, BGE14 and MB11 isolates. Th e SacI digested the isolate MB11, PY12 and the vaccine strain, but it did not cleave the very virulent isolates of IBDV. HhaI cleaved all the isolates with diff erent restriction profi le patterns. StuI digested all the vvIBDV isolates and BspMI was not able to diff erentiate fi eld isolates from vaccine strain. Th ough RT-PCR combined with RFLP is a genotypic method, further confi rmation of serotypes to distinguish the vvIBDV from cvIBDV has to be carried out using pathogenicity studies.Keywords: infectious bursal disease virus; reverse transcription polymerase chain reaction; restriction fragment length polymorphism; VP2 gene; genotyping * Corresponding author. E-mail: tmaskumar@yahoo.com, tmaskumar@tanuvas.org.in; phone: +91-44-25369301. Abbreviations: RT-PCR = reverse transcription PCR; RFLP = restriction fragment length polymorphism; cvIBDV = classical virulent infectious bursal disease virus; vvIBDV = very virulent infectious bursal disease virus

show abstract

Sequence analysis of the VP2 gene hypervariable region of infectious bursal disease viruses from India

Cited by 19 publications

References 28 publications

Molecular Characterization of Infectious Bursal Disease Virus From Commercial Poultry in Pakistan

Molecular Characterization of Infectious Bursal Disease Virus From Commercial Poultry in Pakistan

Characterization of the antigenic, immunogenic, and pathogenic variation of infectious bursal disease virus due to propagation in different host systems (bursa, embryo, and cell culture). I. Antigenicity and immunogenicity

Genotypic characterization of Indian isolates of infectious bursal disease virus strains by reverse transcription-polymerase chain reaction combined with restriction fragment length polymorphism analysis

Contact Info

Product

Resources

About