Sequence and analysis of the mitochondrial DNA control region in the sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

Bravo, Juliana Pereira; Felipes, Joice; Zanatta, Daniela B; Silva, José Luis da Conceição; Fernandez, Maria Aparecida

doi:10.1590/s1516-89132008000400004

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…The rrn L transcript was also present in the highest proportion of D. melanogaster [11] and porcine cistrons [10] , and the analogous large abundance of rrnL transcripts in human expression data was attributed to the presence of a second HSP upstream of the gene that terminates in proximity near the tRNA LeuUUR downstream of the 16 S rRNA [66] , [67] , [68] . The HSP and LSP sequences have diverged rapidly in mammalian systems [69] , such that defining these functional regions is difficult by comparative sequence analyses [70] . The proportional range of cistron levels for those that encode for proteins involved in the same process showed ≤11.15-fold difference in cistron quantities for the NADH dehydrogenase complex ( nd 1 vs. nd 2), and a ≤3.59-fold variance among cistrons for the cytochrome c oxidase ( cox II vs. cox III).…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial Genome Sequence and Expression Profiling for the Legume Pod Borer Maruca vitrata (Lepidoptera: Crambidae)

et al. 2011

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We report the assembly of the 14,054 bp near complete sequencing of the mitochondrial genome of the legume pod borer (LPB), Maruca vitrata (Lepidoptera: Crambidae), which we subsequently used to estimate divergence and relationships within the lepidopteran lineage. The arrangement and orientation of the 13 protein-coding, 2 rRNA, and 19 tRNA genes sequenced was typical of insect mitochondrial DNA sequences described to date. The sequence contained a high A+T content of 80.1% and a bias for the use of codons with A or T nucleotides in the 3rd position. Transcript mapping with midgut and salivary gland ESTs for mitochondrial genome annotation showed that translation from protein-coding genes initiates and terminates at standard mitochondrial codons, except for the coxI gene, which may start from an arginine CGA codon. The genomic copy of coxII terminates at a T nucleotide, and a proposed polyadenylation mechanism for completion of the TAA stop codon was confirmed by comparisons to EST data. EST contig data further showed that mature M. vitrata mitochondrial transcripts are monocistronic, except for bicistronic transcripts for overlapping genes nd4/nd4L and nd6/cytb, and a tricistronic transcript for atp8/atp6/coxIII. This processing of polycistronic mitochondrial transcripts adheres to the tRNA punctuated cleavage mechanism, whereby mature transcripts are cleaved only at intervening tRNA gene sequences. In contrast, the tricistronic atp8/atp6/coxIII in Drosophila is present as separate atp8/atp6 and coxIII transcripts despite the lack of an intervening tRNA. Our results indicate that mitochondrial processing mechanisms vary between arthropod species, and that it is crucial to use transcriptional information to obtain full annotation of mitochondrial genomes.

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Section: Resultsmentioning

confidence: 99%

Mitochondrial Genome Sequence and Expression Profiling for the Legume Pod Borer Maruca vitrata (Lepidoptera: Crambidae)

et al. 2011

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“…The mtDNA control region and ITS have been successfully employed as molecular markers in some studies of population genetic structure (Vandewoestijne et al 2004;Kim et al 2007;Bravo et al 2008;Depaquit et al 2008;Meraner et al 2008;Hamarsheh et al 2009). In this study, we employed the mtDNA A?T-rich region and nuclear ITS2 sequence in order to evaluate, for the first time, the genetic diversity and geographic variations of S. litura.…”

Section: Introductionmentioning

confidence: 99%

DNA Sequence Variation of the Tobacco Cutworm, Spodoptera litura (Lepidoptera: Noctuidae), Determined by Mitochondrial A+T-rich Region and Nuclear ITS2 Sequences

Wan

Kim

et al. 2011

Biochem Genet

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In an effort to gain greater insight into the nature of the population genetic structure of the pest insect Spodoptera litura (Lepidoptera: Noctuidae), tobacco cutworms were collected from six Korean and five Chinese localities, and their mitochondrial A+T-rich region and nuclear internal transcribed spacer 2 (ITS2) were cloned and sequenced. The A+T-rich region and nuclear ITS2 provided a maximum sequence divergence of 2.88 and 1.82%, respectively. Overall, a low level of genetic fixation (F(ST) = 0-0.02965 in the A+T-rich region and 0-0.34491 in ITS2) and no discernible isolated population were noted among most S. litura populations. Along with these results, the absence of genetic variance between Korea and China indicates a profound interrelation of the S. litura populations in the two countries, consistent with the current notion that S. litura has sufficient flight capacity for dispersal.

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“…Our research group recently described the Diatraea saccharalis mitochondrial control region, CR (Bravo et al, 2008). Sequence analysis demonstrated that this region of the D. saccharalis mitochondrial genome has high similarity with that of the Lepidopteran Cydia pomonella; however, these results did not clarify the taxonomic problem posed by D. saccharalis.…”

Section: Diatraea Saccharalis Barcodementioning

confidence: 94%

ReviewDNA barcode information for the sugar cane moth borer Diatraea saccharalis

Bravo¹,

Silva²,

Munhoz³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. We reviewed the use and relevance of barcodes for insect studies and investigated the barcode sequence of Diatraea saccharalis. This sequence has a high level of homology (99%) with the barcode sequence of the Crambidae (Lepidoptera). The sequence data can be used to construct relationships between species, allowing a multidisciplinary approach for taxonomy, which includes morphological, molecular and distribution data, all of which are essential for the understanding of biodiversity. The D. saccharalis barcode is a previously undescribed sequence that could be used to analyze Lepidoptera biology.

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Sequence and analysis of the mitochondrial DNA control region in the sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

Abstract: This study aimed at the sequence and analysis of the mtDNA control region (CR) of the

Cited by 7 publications

References 23 publications

Mitochondrial Genome Sequence and Expression Profiling for the Legume Pod Borer Maruca vitrata (Lepidoptera: Crambidae)

Mitochondrial Genome Sequence and Expression Profiling for the Legume Pod Borer Maruca vitrata (Lepidoptera: Crambidae)

DNA Sequence Variation of the Tobacco Cutworm, Spodoptera litura (Lepidoptera: Noctuidae), Determined by Mitochondrial A+T-rich Region and Nuclear ITS2 Sequences

ReviewDNA barcode information for the sugar cane moth borer Diatraea saccharalis

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