Sequence and expression of four coral G protein-coupled receptors distinct from all classifiable members of the rhodopsin family

Anctil, Michel; Hayward, David C.; Miller, David J.; Ball, Eldon E.

doi:10.1016/j.gene.2006.10.025

Cited by 24 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is composed of 538 non-redundant (identity < 90%), non-olfactory sequences, 26% of which could be assigned to GPCR sub-families present in humans. The remaining sequences belong to GPCR sub-families specific of cnidarians [53]. The accession numbers of the sequences used for this study are given in Data S1.…”

Section: Methodsmentioning

confidence: 99%

Multidimensional Scaling Reveals the Main Evolutionary Pathways of Class A G-Protein-Coupled Receptors

et al. 2011

View full text Add to dashboard Cite

Class A G-protein-coupled receptors (GPCRs) constitute the largest family of transmembrane receptors in the human genome. Understanding the mechanisms which drove the evolution of such a large family would help understand the specificity of each GPCR sub-family with applications to drug design. To gain evolutionary information on class A GPCRs, we explored their sequence space by metric multidimensional scaling analysis (MDS). Three-dimensional mapping of human sequences shows a non-uniform distribution of GPCRs, organized in clusters that lay along four privileged directions. To interpret these directions, we projected supplementary sequences from different species onto the human space used as a reference. With this technique, we can easily monitor the evolutionary drift of several GPCR sub-families from cnidarians to humans. Results support a model of radiative evolution of class A GPCRs from a central node formed by peptide receptors. The privileged directions obtained from the MDS analysis are interpretable in terms of three main evolutionary pathways related to specific sequence determinants. The first pathway was initiated by a deletion in transmembrane helix 2 (TM2) and led to three sub-families by divergent evolution. The second pathway corresponds to the differentiation of the amine receptors. The third pathway corresponds to parallel evolution of several sub-families in relation with a covarion process involving proline residues in TM2 and TM5. As exemplified with GPCRs, the MDS projection technique is an important tool to compare orthologous sequence sets and to help decipher the mutational events that drove the evolution of protein families.

show abstract

Section: Methodsmentioning

confidence: 99%

Multidimensional Scaling Reveals the Main Evolutionary Pathways of Class A G-Protein-Coupled Receptors

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Acropora millepora embryos were processed as described by Anctil et al [52] up to BCIP/NBT color development. Color development was then stopped by washing several times with PBT, and the backgrounds cleared in ethanol.…”

Section: Methodsmentioning

confidence: 99%

Sox genes in the coral Acropora millepora: divergent expression patterns reflect differences in developmental mechanisms within the Anthozoa

et al. 2008

View full text Add to dashboard Cite

BackgroundSox genes encode transcription factors that function in a wide range of developmental processes across the animal kingdom. To better understand both the evolution of the Sox family and the roles of these genes in cnidarians, we are studying the Sox gene complement of the coral, Acropora millepora (Class Anthozoa).ResultsBased on overall domain structures and HMG box sequences, the Acropora Sox genes considered here clearly fall into four of the five major Sox classes. AmSoxC is expressed in the ectoderm during development, in cells whose morphology is consistent with their assignment as sensory neurons. The expression pattern of the Nematostella ortholog of this gene is broadly similar to that of AmSoxC, but there are subtle differences – for example, expression begins significantly earlier in Acropora than in Nematostella. During gastrulation, AmSoxBb and AmSoxB1 transcripts are detected only in the presumptive ectoderm while AmSoxE1 transcription is restricted to the presumptive endoderm, suggesting that these Sox genes might play roles in germ layer specification. A third type B Sox gene, AmSoxBa, and a Sox F gene AmSoxF also have complex and specific expression patterns during early development. Each of these genes has a clear Nematostella ortholog, but in several cases the expression pattern observed in Acropora differs significantly from that reported in Nematostella.ConclusionThese differences in expression patterns between Acropora and Nematostella largely reflect fundamental differences in developmental processes, underscoring the diversity of mechanisms within the anthozoan Sub-Class Hexacorallia (Zoantharia).

show abstract

“…corals and identified as cryptochromes and opsins, which absorbs mainly blue light (Levy et al, 2007;Mason et al, 2012). Furthermore, corals express rhodopsins and melanopsins photoreceptors (Gorbunov and Falkowski, 2002;Anctil et al, 2007;Vize, 2009) with a maximal sensitivity in blue light. These photoreceptors transfer signals via calcium as second messenger in many marine invertebrates (Yarfitz and Hurley, 1994;Vize, 2009).…”

Section: Introductionmentioning

confidence: 99%

Light Enhanced Calcification in Hermatypic Corals: New Insights from Light Spectral Responses

2016

View full text Add to dashboard Cite

Light enhanced calcification (LEC) is a well-documented phenomenon in reef-building corals. The main mechanism proposed for LEC is that photosynthetic CO 2 uptake by the algal symbionts elevates the pH and thus enhances calcification. We evaluated the role of light and of photosynthesis on calcification by assessing the response of the corals Porites lutea and Acropora variabilis to different components of the light spectrum. Calcification and photosynthesis of both species decreased under "lagoon" blue, green and red light (peaks at 500, 550, and 600 nm, respectively). However, blue light (peak at 455 nm) enhanced calcification rates of P. lutea and A. variabilis (up to 4.1-and 10.5-fold of dark values, respectively) reaching levels comparable to those measured under full spectrum illumination. However, contrary to our expectations, photosynthetic oxygen production was considerably reduced under blue light, to the extent that it remained below the compensation point even under illumination as high as 400 µmol photons m −2 s −1. It is the first time that a direct effect of light not mediated by the photosynthetic process has been demonstrated to trigger LEC in corals. We propose that blue light signaling, and animal receptors thereof may be involved in the enhancement of calcification by hermatypic corals.

show abstract

Sequence and expression of four coral G protein-coupled receptors distinct from all classifiable members of the rhodopsin family

Cited by 24 publications

References 42 publications

Multidimensional Scaling Reveals the Main Evolutionary Pathways of Class A G-Protein-Coupled Receptors

Multidimensional Scaling Reveals the Main Evolutionary Pathways of Class A G-Protein-Coupled Receptors

Sox genes in the coral Acropora millepora: divergent expression patterns reflect differences in developmental mechanisms within the Anthozoa

Light Enhanced Calcification in Hermatypic Corals: New Insights from Light Spectral Responses

Contact Info

Product

Resources

About